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Molecular cloning of the human geneSUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation

Abstract

DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring a normal DPC response and solar UV sensitivity to DRP 153. This gene has been designatedSUVCC3 to denote solar UV cross-complementing gene number 3.

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Rosenstein, B.S., Vaslet, C.A. Molecular cloning of the human geneSUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation. Somat Cell Mol Genet 21, 255–263 (1995). https://doi.org/10.1007/BF02255780

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Keywords

  • Molecular Cloning
  • Gene Number
  • Human Gene
  • Recombinant Phage
  • Secondary Transformant