DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring a normal DPC response and solar UV sensitivity to DRP 153. This gene has been designatedSUVCC3 to denote solar UV cross-complementing gene number 3.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Chiu, S.-M., Sokany, N.M., Friedman, L.R., and Oleinick, N.L. (1984). Differential processing of ultraviolet or ionising radiation-induced DNA-protein crosslinks in Chinese hamster cells.Int. J. Rad. Biol. 46:681–690.
Matsumoto, K., Sugiyama, M., and Ogura, R. (1991). Non-dimer DNA damage in Chinese hamster V-79 cells exposed to ultraviolet-B light.Photochem. Photobiol. 54:389–392.
Rosenstein, B.S., Lai, L.-W., Ducore, J.W., and Rosenstein, R.B. (1989). DNA-protein crosslinking in normal and solar UV-sensitive ICR 2A frog cell lines exposed to solar UV-radiation.Mutation Res. 217:219–226.
Lai, L.-W., Ducore, J.W., and Rosenstein, B.S. (1987). DNA-protein crosslinking in normal human skin fibroblasts exposed to solar ultraviolet wavelengths.Photochem. Photobiol. 46:143–146.
Rosenstein, B.S., Rosenstein, R.B., and Zamansky, G.B. (1992). Repair of DNA damage induced in systemic lupus erythematosus skin fibroblasts by simulated sunlight.J. of Invest. Dermatol. 98:469–474, 1992.
Rosenstein, B.S. (1984). Photoreactivation of ICR 2A frog cells exposed to solar UV wavelengths.Photochem. Photobiol. 40:207–213.
Rosenstein, B.S. (1982). The action spectra for photoreactivation and photorepair in ICR 2A frog cells.Photochem. Photobiol. 35:491–494.
Setlow, R.B., and Regan, J.D. (1981). Measurement of repair synthesis by photolysis of bromouracil. InDNA Repair, Vol. 1, part B, (eds.) Friedberg, E.C., and Hanawalt, P.C. (Marcel Dekker, New York), pp. 307–318.
Rosenstein, B.S., Murphy, J.T., and J.D. Ducore (1985). Use of a highly sensitive assay to analyze the excision repair of dimer and non-dimer DNA damages induced in human skin fibroblasts by 254 nm and solar ultraviolet radiation.Cancer Res. 45:5526–5531.
Kantor, G.J., Warner, C., and Hull, D.R. (1977). The Effect of ultraviolet light on arrested human diploid cell populations.Photochem. Photobiol. 25:483–489.
Kohn, K.W., Ewig, R.A.G., Erickson, L.C., and Zwelling, L.A. (1981). Measurement of strand breaks and cross-links by alkaline elution. InDNA Repair, Vol. 1, part B (eds.) Friedberg, E.C., and Hanawalt, P.C. (Marcel Dekker, New York), pp. 379–401.
Kohn, K.W., Erickson, L.C., Ewig, R.A.G., and Friedman, C.A. (1976). Fractionation of DNA from mammalian cells by alkaline elution.Biochemistry 15:4629–4637.
Cesarone, C.F., Bolognesi, C., and Santi, L. (1979). Improved microfluorometric DNA determination in biological material using 33258 Hoechst.Anal. Biochem. 110:188–197.
Stout, D.L., and Becker, F.F. (1982). Fluorometric quantitation of single-stranded DNA: A method applicable to the technique of alkaline elution.Anal. Biochem. 127:302–307.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1989).Current Protocols in Molecular Biology, John Wiley, New York.
Graham, F.L., and van der Eb, A.J. (1973). A new technique for the assay of infectivity of human adenovirus 5 DNA.Virology 52:456–467.
Wigler, M., Pellicer, A., Silverstein, S., and Axel, R. (1978). Biochemical transfer of single-copy eukaryotic genes using total cellular DNA as donor.Cell 14:725–731.
Southern, P.J., and Berg, P. (1982). Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.J. Mol. Appl.
SAS Institute Inc. (1988).SAS/STAT Users Guide, Release 6.03 Edition, SAS Institute Inc., Cary, North Carolina.
Hall, E.J. (1994).Radiobiology for the Radiobiologist, J.B. Lippincott, Philadelphia.
Perucho, M., Goldfarb, M., Shimizu, K., Lama, C., Fogh, J., and Wigler, M. (1981). Human-tumorderived cell lines contain common and different transforming genes.Cell 27:467–476.
Gusella, J.F., Keys, C., Varsanyi-Breiner, A., Kao, F.-T., Jones, C., Puck, T.T., and Housman, D. (1980). Isolation and localization of DNA segments from specific human chromosomes.Proc. Natl. Acad. Sci. U.S.A. 77:2829–2833.
Rosenstein, B.S., Vaslet, C.A., and Rosenstein, R.B. (1995). Molecular cloning of the human geneSUVCC1 associated with the repair of non-dimer DNA damages induced by solar UV radiation.Photochem. Photobiol. 61:142–148.
Rosenstein, B.S., and Vaslet, C.A. (1995). Molecular cloning of the human geneSUVCC2 associated with mutagenesis following the induction of nondimer DNA damages induced by solar UV radiation.J. Photochem. Photobiol. 28:203–211.
Troelstra, C., Odijk, H., de Wit, J., Westerveld, A., Thompson, L.H., Bootsma, D., and Hoeijmakers, J.H.J. (1990). Molecular cloning of the human DNA excision repair geneERCC-6.Mol. Cell. Biol. 10:5806–5813.
Weeda, G., van Ham, R.C.A., Masurel, R., Westerveld, A., Odijk, H., de Wit, J., van der Eb, A.J., and Hoeijmakers, J.H.J. (1990) Molecular cloning and biological characterization of the human excision repair geneERCC-3.Mol. Cell. Biol. 10:2570–2581.
Weeda, G., Hoeijmakers, J.H., and Bootsma, D. (1993). Genes controlling nucleotide excision repair in eukaryotic cells.Bioessays 15:249–258.
About this article
Cite this article
Rosenstein, B.S., Vaslet, C.A. Molecular cloning of the human geneSUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation. Somat Cell Mol Genet 21, 255–263 (1995). https://doi.org/10.1007/BF02255780
- Molecular Cloning
- Gene Number
- Human Gene
- Recombinant Phage
- Secondary Transformant