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Chromosome Research

, Volume 4, Issue 1, pp 24–28 | Cite as

Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescencein situ hybridization (FISH)

  • Xiao-Bo Zhong
  • J. Hans de Jong
  • Pim Zabel
Article

Abstract

Fluorescencein situ hybridization (FISH) is an increasingly powerful tool with a variety of applications in both basic and applied research. With excellent genetic, cytogenetic and molecular maps available, the tomato genome provides a good model to benefit from the full potential of FISH. Tomato chromosomes at mitotic metaphase are small and not particularly suitable for high-resolution FISH. In contrast, chromosomes at meiotic pachytene are about 15 times longer, and easier to identify by their differences in chromosome arm lengths and chromomere pattern. We have developed a technique for preparing chromosomal spreads of young pollen mother cells at midprophase I which is suitable for FISH. In a first series of experiments, the hybridization patterns of three classes of repetitive DNA sequences were studied in single and multicolour FISH.

Key words

fluorescencein situ hybridization pachytene metaphase repetitive sequences tomato 

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Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  • Xiao-Bo Zhong
    • 1
  • J. Hans de Jong
    • 2
  • Pim Zabel
    • 1
  1. 1.Department of Molecular BiologyWageningen Agricultural UniversityWageningenThe Netherlands
  2. 2.Department of GeneticsWageningen Agricultural UniversityWageningenThe Netherlands

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