Somatic Cell and Molecular Genetics

, Volume 20, Issue 3, pp 147–152 | Cite as

Electron microscopic analysis of in vitro replication products ofors 8, a mammalian origin enriched sequence

  • Christopher E. Pearson
  • Awatef Shihab-El-Deen
  • Gerald B. Price
  • Maria Zannis-Hadjopoulos
Article
Received:
Accepted:

Abstract

Electron microscopy was used to map the initiation site of ors 8 DNA replication in vitro in a system that is capable of initiating and supporting one round of semiconservative replication of cloned mammalian DNA origin-enriched sequences (ors). Using unique restriction sites in ors 8 plasmid DNA, we have mapped the replication bubble within the monkey DNA sequence. In addition to site-specific initiation within the ors, the results also indicate bidirectional replication.

Keywords

Electron Microscopy Restriction Site Microscopic Analysis Initiation Site Electron Microscopic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Christopher E. Pearson
    • 1
  • Awatef Shihab-El-Deen
    • 1
  • Gerald B. Price
    • 1
  • Maria Zannis-Hadjopoulos
    • 1
  1. 1.McGill Cancer Centre, Department of MedicineMcGill UniversityMontrealCanada

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