Inflammation Research

, Volume 45, Issue 9, pp 442–447 | Cite as

Characteristics of the histamine release from hamster cheek pouch mast cells stimulated by lectins from Brazilian beans and concanavalin A

  • R. R. Ferreira
  • B. S. Cavada
  • R. A. Moreira
  • J. T. A. Oliveira
  • J. C. Gomes
Original Research Papers
Received:
Revised:
Accepted:

Abstract

We have characterized the histamine releasing effects of lectins extracted from Brazilian beans, in comparison to concanavalin A, in hamster cheek pouch cell suspensions containing mast cells. The lectins fromDioclea virgata, Canavalia brasiliensis, andDioclea rostrata induce histamine release in a similar manner to concanavalin A, but appear to differ in potency and efficacy. The effects depended on the temperature, pH, and metabolic energy, demonstrating the non-cytotoxic nature of the histamine release. It is suggested that the lectins studied act by the same mechanism as concanavalin A (interacting with sugars in the antibodies bound to the mast cells), since high concentrations of glucose inhibit the histamine release. The lectins at high concentrations quench the histamine release. This suppression is reversed by increasing calcium concentration, suggesting that the lectins bind to the calcium that is essential for the secretion, thereby confirming and extending our previous data using the lectin fromDioclea virgata in rat peritoneal mast cells.

Key words

Histamine release Lectins Mast cells Hamster Cheek pouch 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Lis H, Sharon N. The biochemistry of plant lectins. Ann Rev Biochem 1973;42:541–74.CrossRefPubMedGoogle Scholar
  2. [2]
    Lagunoff D, Martin TW, Read G. Agents that release histamine from mast cells. Ann Rev Pharmacol Toxicol 1983;23:331–51.CrossRefGoogle Scholar
  3. [3]
    Keller R. Concanavalin A, a model “antigen” for the in vitro detection of cell-bound reaginic antibody rat. Clin Exp Immunol 1973;13:139–47.PubMedGoogle Scholar
  4. [4]
    Shibasaki M, Sumazaki R, Isoyama S, Takita H. Interaction of lectins with human IgE: IgE-binding property and histamine-releasing activity of twelve plant lectins. Int Arch Allergy Immunol 1992;98:18–25.PubMedGoogle Scholar
  5. [5]
    Siraganian PA, Siraganian RP. Basophil activation by concanavalin A: characteristics of the reaction. J Immunol 1974;112:2117–25.PubMedGoogle Scholar
  6. [6]
    Shores AJ, Mogar JL. Modulation of histamine secretion from concanavalin A-activated rat mast cells by phosphatidyl serine, calcium, cAMP pH and metabolic inhibitors. Agents Actions 1980;10:131–7.CrossRefPubMedGoogle Scholar
  7. [7]
    Gomes JC, Ferreira RR, Cavada BS, Moreira RA, Oliveira JTA. Histamine release induced by glucose (mannose)-specific lectins isolated from Brazilian beans. Comparison with concanavalin A. Agents Actions 1994;41: 132–5.CrossRefPubMedGoogle Scholar
  8. [8]
    Ishizaka T, Ishizaka K. Mechanisms of passive sensitization. IV. Dissociation of IgE molecules from basophil receptors at acid pH. J Immunol 1974;112:1078–84.PubMedGoogle Scholar
  9. [9]
    Shore PA, Burkhalter A, Cohn JH. A method for the fluorometric assay of histamine in tissue. J Pharmacol Exp Ther 1959;127:182–90.PubMedGoogle Scholar
  10. [10]
    Uvnäs B, Thon IL. Evidence for enzymatic histamine release from isolated rat mast cells. Exp Cell Res 1961;23:45–7.CrossRefPubMedGoogle Scholar
  11. [11]
    Chakravarty N. Metabolic changes in mast cell associated with histamine release. In: Rocha e Silva M, editor. Histamine II and anti-histaminics. Chemistry, Metabolism and physiological and pharmacological actions. Handbook of Experimental Pharmacology. vol. XVIII/2. Berlin, Heidelberg, New York: Springer 1978:91–108.Google Scholar
  12. [12]
    Chakravarty N. The role of plasma membrane Ca++−Mg++ activated adenosine triphosphatase of rat mast cells on histamine release. Acta Pharmacol Toxicol 1980;47:223–35.Google Scholar
  13. [13]
    Johansen T. Energy metabolism in rat mast cells in relation to histamine secretion. Pharmacol Toxicol 1987;61 Suppl II:1–20.Google Scholar
  14. [14]
    Magro AM, Bennich H. Concanavalin A induced histamine release from human basophils in vitro. Immunology 1977;33:51–8.PubMedGoogle Scholar
  15. [15]
    Landry Y, Bronner C, Mousli M, Fischer T, Valle A. The activation of mast cells — Molecular Targets and Transducing Processes for Antigenic and Nonantigenic Stimuli. Bull Inst Pasteur 1992;90:83–98.Google Scholar
  16. [16]
    Hook WA, Dougherty SF, Oppenheim JJ. Release of histamine from hamster mast cells by concanavalin and phytohemagglutinin. Infect Immun 1974;9:903–8.PubMedGoogle Scholar
  17. [17]
    Morita Y, Matsumoto Y, Miyamoto T, Horiuchi Y. A clinical study of concanavalin A-induced histamine release utilizing a single isotopic enzymatic assay of histamine. Ann Allergy 1978;40:47–50.PubMedGoogle Scholar
  18. [18]
    Moreira RAM, Ainouz IL, Oliveira JTA, Cavada BS. Plant lectins, chemical and biological aspects. Mem Inst Oswaldo Cruz 1991;86 Suppl II:211–8.PubMedGoogle Scholar
  19. [19]
    Leung KBP, Pearce FL. A comparison of histamine secretion from peritoneal mast cells of the rat and hamster. Br J Pharmacol 1984;81:693–701.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1996

Authors and Affiliations

  • R. R. Ferreira
    • 1
  • B. S. Cavada
    • 2
  • R. A. Moreira
    • 2
  • J. T. A. Oliveira
    • 2
  • J. C. Gomes
    • 1
  1. 1.Department of PharmacologyInstitute of BiosciencesBotucatuBrazil
  2. 2.Department of Biochemistry and Molecular BiologyFederal University of CearáFortalezaBrazil

Personalised recommendations