, Volume 121, Issue 4, pp 485–487 | Cite as

Vesamicol, an acetylcholine uptake blocker in presynaptic vesicles, suppresses rapid eye movement (REM) sleep in the rat

  • R. J. Salin-Pascual
  • A. Jimenez-Anguiano
Original Investigation


Vesamicol inhibits acetylcholine uptake in presynaptic vesicles and reduces its release. The present study was performed in order to test the effects of this drug in a cholinergic related function as rapid eye movement (REM) sleep. Wistar male rats were implanted for sleep recordings. In addition, a stainless steel cannula was implanted into the left lateral ventricle for intracerebroventricular (ICV) injections. In experiment 1, a dose-response curve was performed. Saline or vesamicol (20, 40, 80 and 100 µg) were injected. Following the ICV injections, animals' sleep was recorded for 8 h. In experiment 2, after adaptation and baseline recordings, animals received 50 µg vesamicol ICV at 1000 hours. every 24 h for 2 consecutive days. After each injection an 8-h sleep recording session was performed. Two subsequent recovery recordings were allowed. Results obtained in experiment 1 showed a dose-response reduction of REM sleep with significant values at 80 µg and 100 µg of vesamicol. The main findings in experiment 2 were a reduction in REM sleep time and an increases in REM sleep latency. On the recovery days, a dramatic rebound of REM sleep was observed. Vesamicol behaved as an anticholinergic drug. It produced a reduction in REM sleep time and a rebound of this sleep stage after its withdrawal.

Key words

Vesamicol Acetylcholine REM sleep Sleep Rats 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • R. J. Salin-Pascual
    • 1
  • A. Jimenez-Anguiano
    • 2
  1. 1.Departamento de fisiologia, Facultad de MedicinaUniversidad Nacional Autonoma de MexicoMexico
  2. 2.Instituto de Fisiologia CelularUniversidad Nacional Autonoma de MexicoMexico

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