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Tropical Animal Health and Production

, Volume 12, Issue 1, pp 50–60 | Cite as

A comparison of the complement fixation and immunofluorescent antibody tests in a survey of the prevalence ofBabesia equi andBabesia caballi in horses in the sultanate of Oman

  • J. Donnelly
  • L. P. Joyner
  • Oliver Graham-Jones
  • C. P. Ellis
Article

Summary

The incidence of antibodies toBabesia equi andB. caballi in horses in the Royal Stables of His Majesty the Sultan of Oman was assessed by complement fixation (CF) and immunofluorescent antibody (IFA) tests. Two series of samples taken with a 2-year interval, mainly from animals reared in Oman, indicated a stable but high prevalence of antibodies. On the 2 occasions 94.6 and 97.7% respectively were positive toB. equi by IFA and 76.8 and 75.0% were positive by CF. ForB. caballi the corresponding percentage figures were lower—67.9 and 40.9 by IFA and 30.4 and 40.9 by CF. A group of animals tested within 4 months of importation gave 60.4% positive reactions by IFA and 62.5% by CF toB. equi; 6.3% positive toB. caballi by IFA and 8.3% by CF. These figures are consistent with the probable relative prevalence of the parasites. The discrepancy between the results obtained by the 2 serological tests was probably due to the greater sensitivity of the IFA test and the faster decay of the antibodies detectable by the CF test.

Keywords

Oman Positive Reaction Serological Test Antibody Test Fast Decay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Comparison Entre Les Tests De Fixation Du Complement Et De Recherche Des Anticorps Immunofluorescents Dans La Mise En Evidence DeBabesia Equi EtBabesia Caballi Chez Les Chevaux Du Sultanat D'oman

Résumé

La présence d'anticorps contreBabesia equi etBabesia caballi chez les chevaux des Ecuries Royales de sa majesté le Sultan d'Oman a été recherchée par les tests de fixation du complément (CF) et des anticorps fluorescents (IFA). Deux séries d'échantillons prélevées à deux ans d'intervalle, le plus souvent sur les chevaux élevés à Oman, ont montré une régulière mais haute dominance des anticorps. En ces deux occasions, l'IFA a donné 94,6 et 97,7 p. 100 de résultats positifs en ce qui concerneB. equi et CF 76,8 et 75 p. 100. PourB. caballi les pourcentages correspondant sont plus bas 67,9 et 40,9 par IFA et 30,4 et 40,9 par CF. Un groupe d'animaux éprouvés dans les quatre mois qui ont suivi leur importation ont donné 60,4 p. 100 de réactions positives par IFA et 62,5 p. 100 par CF à l'égard deB. equi; 6,3 p. 100 étaient positifs àB. caballi par IFA et 8,3 p. 100 par CF. Ces résultats sont en relation avec la fréquence relative des parasites. La différence installée entre les résultats obtenus par les deux épreuves sérologiques est probablement due à la plus grande sensibilité de l'IFA et la plus rapide décroissance des anticorps décelables par le test CF.

Comparacion De Las Pruebas De Fijacion De Complemento Y Anticuerpos Inmunofluorescentes En Un Reconocimiento De La Prevalencia DeBabesia Equi yBabesia Caballi En Equinos Del Sultanato De Oman

Resumen

Se utilizaron las pruebas de fijación de complemento y la de anticuerpos fluorescentes para detectar la prevalencia de anticuerpos deBabesia equi yBabesia caballi en equinos de los establos reales de su Majestad el Sultán de Omán. Dos series de sueros tomados con un intervalo de 2 años, de caballos criados en Omán, revelaron una prevalencia de anticuerpos constante pero alta. En ambas ocasiones 94.6 y 97.7% de los sueros respectivamente fueron positivos aB. equi mediante la prueba de inmunofluorescencia y 76.8 y 75.0% fueron positivos a la fijación de complemento. ParaB. caballi los porcentajes correspondientes fueron más bajos 67.9 y 40.9 por inmunofluorescencia y 30.4 y 40.9 por fijación de complemento. Un grupo de animales examinados dentro de los 4 meses de la importación tuvo una positividad del 60.4% mediante la prueba de inmunofluorescencia y 62.5% por fijación de complemento aB. equi; 6.3% fueron positivos aB. caballi por inmunofluorescencia y 8.3% por fijación de complemento. Estos datos son consistentes con la probable prevalencia relativa de los parásitos. La discrepancia entre los resultados obtenidos mediante el uso de las dos pruebas serológicas se debió probablemente a la mayor sensibilidad de la prueba de anticuerpos fluorescentes y a la caída rápida de los anticuerpos fijadores de complemento.

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Copyright information

© Longman Group Limited 1980

Authors and Affiliations

  • J. Donnelly
    • 1
  • L. P. Joyner
    • 1
  • Oliver Graham-Jones
    • 2
  • C. P. Ellis
    • 3
  1. 1.Ministry of Agriculture Fisheries and Food, Central Veterinary Laboratory, New HawWeybridge
  2. 2.Veterinary Advisor to HM The Sultan of OmanUK
  3. 3.Department of Veterinary Services, The Palace, Muscat, Sultanate of OmanUK

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