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Hypocalcemia response following calcitonin administration: Lack of correlation with osteoclast number determined after histoenzymologic identification in osteoprorosis

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The relationship between calcitonin-induced hypocalcemia and histomorphometric parameters of bone resorption was examined in iliac crest biopsies of 30 osteoporotic patients aged 55–86 years all of whom had received a single injection of 100 UI of salmon calcitonin. Number of osteoclasts and active resorption surfaces were determined after histoenzymologic staining based on osteoclastic tartrate resistant acid phosphatase content. No significant correlation could be demonstrated between the drop of hypocalcemia and the different histomorphometric parameters. It can be concluded that the calcitonin test is useless in osteoporosis.

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  1. 1.

    Fournié, A., Valvelde, C., Tap, G., Bazerque, J.J., Ayrolles, Ch., Test d'hypocalcémie aigue à la calcitonine de porc et de saumon. Rev Rhum 1977, 44, 91–98.

  2. 2.

    Alexandre, C., Chapuy, M.C., Meunier, P. Le test d'hypocalcémie aux calcitonines synthétiques de saumon et humaine. Rev Rhum 1979, 46, 335–342.

  3. 3.

    Bataille, R., Sany, J. Clinical evaluation of myeloma osteoclastic bone lesions: II. Induced hypocalcemia test using salmon calcitonin. Metab Bone Dis Relat Res 1982, 4, 39–42.

  4. 4.

    Benson, L., Wide, L., Akerstrom, G., Ljunghall, S. A stimulation test with calcitonin for differential diagnosis of hypercalcemia. Acta Endocrinol 1984, 107, 237–244.

  5. 5.

    Bordier, P., Hioco, D., Tun Chot, S. Calcitonin: acute effects upon serum calcium urinary hydroxyproline excretion and osteoclasts in man. In: Calcitonin Proceeding of the II International Symposium. Editor: Taylor, S., Foster, G. London, W. Heineman Medical Books, 1969, 339–348.

  6. 6.

    Ste-Marie, L.G., Charhon, S.A., Meunier, P.J. Le test d'hypocalcémie à la calcitonine a-t-il une utilité dans les ostéoporoses? Presse Méd. 1983, 12, 2883–2884.

  7. 7.

    Chappard, D., Alexandre, C., Riffat, G. Histochemical identification of osteoclasts. Review of current methods and reappraisal of a simple procedure for routine diagnosis on undecalcified human iliac bone biopsies. Basic Appl Histochem 1983, 27, 75–85.

  8. 8.

    Evans, R.A., Dunstan, C.R., Baylink, D.J. Histochemical identification of osteoclasts in undecalcified sections of human bone. Min Elect Metab 1979, 2, 179–185.

  9. 9.

    Chappard, D., Alexandre, C., Bousquet, G., Riffat, G. Nouvelles modifications du trocart de Bordier pour la biopsie osseuse quantitative. Rev Rhum 1983, 50, 307–310.

  10. 10.

    Chappard, D., Alexandre, C., Camps, M., Montheard, J.P., Riffat, G. An embedding process of iliac bone biopsies at low temperature using glycol and methyl methacrylate. Stain Technol 1983, 58, 299–303.

  11. 11.

    Chappard, D., Palle, S., Alexandre, C., Vico, L., Riffat, G. Bone embedding in pure methacrylates at low temperature preserves enzyme activities. Acta Histochem 1987, 81, 183–190.

  12. 12.

    Chappard, D., Palle, S., Alexandre, C., Vico, L., Riffat, G. Simultaneous identification of calcified cartilage, bone and osteoid tissue on plastic sections. J Histotechnol 1986, 9, 95–99.

  13. 13.

    Johnell, O., Wiklund, P.E., Hulth, A. Osteoclast counting in crista biopsies. Acta Orthop Scand 1977, 48, 566–571.

  14. 14.

    Malluche, H. H., Meyer, W., Sherman, D., Massry, S.G. Quantitative bone histology in 84 normal American subjects. Calcif Tissue Int 1982, 34 449–455.

  15. 15.

    Vico, L., Chappard, D., Alexandre, C., Palle, S., Minaire, P., Riffat, G., Novikov, V.E., Bakulin, A.V. Effects of weightlessness on bone mass and osteoclast number in pregnant rats after a five-day spaceflight (Cosmos 1514). Bone 1987, 8, 95–103.

  16. 16.

    Colson, S., Gioud, M., Tebib, J.G., Matthieu, M., Bouvier, M. Le test dynamique d'hypocalcémie induite par la calcitonine comme moyen d'évaluation globale de l'activité ostéoclastique in vivo: nouvelle méthode d'interprétation des résultats dans le démembrement des ostéoporoses. Rev Rhum 1987, 54, 481–486.

  17. 17.

    Meunier, P.J., Sellami, S., Briançon, D., Edouard, C. Histological heterogeneity of apparently idiopathic osteoporosis. In: Osteoporosis. Recent Advances in Pathogenesis and Treatment. Editor: Deluca, H.F. et al, University Park Press, Baltimore, 1981, 293–301.

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Correspondence to S. Palle.

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Palle, S., Chappard, D., Vico, L. et al. Hypocalcemia response following calcitonin administration: Lack of correlation with osteoclast number determined after histoenzymologic identification in osteoprorosis. Clin Rheumatol 7, 354–358 (1988). https://doi.org/10.1007/BF02239192

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Key words

  • Calcitonin
  • Bone Histomorphometry
  • Tartrate Resistant Acid Phosphatase