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Theileria parva carrier state in naturally infected and artificially immunised cattle

L'etat De Porteur DeTheileria parva Chez Des Bovins Naturellement Infectes Et Immunises Artificiellement

El Estado De Portador DeTheileria Parva En Animales Infectados En Forma Natural Y Artificialmente Mediante Inmunizacion

Summary

Rhipicephalus appendiculatus adult ticks, collected in the field from zebu (Bos indicus) and exotic (Bos tarus) cattle with high antibody titres toTheileria parva schizont antigen, transmittedTheileria parva infection typical of East Coast fever to susceptible cattle. UninfectedR. appendiculatus nymphs applied to naturally recovered zebu and exotic cattle kept under tick-free conditions in the laboratory for 16 and 7 months respectively, transmitted fatal theileriosis to susceptible cattle. Cattle immunised by the infection and treatment method were shown to be carriers ofTheileria parva by examination of the salivary glands of ticks applied to them and by tick transmission. Three and 7 months after immunisation,Theileria parva infected lymphocytes were establishedin vitro from peripheral blood lymphocytes. This was the first demonstration,in vitro, of the existence of schizonts inTheileria parva (East Coast fever) infection carrier status.

These studies show that cattle from endemic and epidemic areas of East Coast fever (ECF) become carriers thereby maintaining theT. parva population. The relevance of the findings in this study to the control of ECF by dipping, immunisation and treatment is discussed.

Résumé

Des tiques adultes deRhipicephalus appendiculatus récoltées sur des zébus (Bos indicus) et des bovins importés (Bos taurus) présentant des taux élevés d'anticorps vis à vis d'antigènes de schizontes deTheileria parva, ont transmis une infection àTh. parva typique de theileriose à des bovins sensibles. Des nymphes deR. appendiculatus non infectées, appliquées sur des zébus naturellement guéris et placées ultérieurement sur des bovins importés maintenus à l'abri des tiques pendant 16 et 17 mois respectivement on transmis une theileriose mortelle aux bovins sensibles. Les bovins immunisés par la méthode infection et traitement se sont révélés porteurs deTh. parva par l'examen des glandes salivaires de tiques fixées sur eux et par la transmission par les tiques, et 3 et 7 mois après immunisation des lymphocytes infectés parTh. parva ont été mis en évidencein vitro à partir des lymphocytes du sang periphérique. Il s'agit de la première observation,in vitro, de l'existence de schizontes chez des animaux porteurs de theileriose àTh. parva. Ces études montrent que les bovins des régions où sévit la theileriose à l'état épidémique où endémique deviennent porteurs, maintenant ainsi la population deTh. parva. Les observations lors de cette étude concernant la lutte contre la theileriose par l'utilisation de bains détiqueurs, l'immunisation et le traitement sont discutées.

Resumen

Garrapatas adultasRhipicephalus appendiculatus, recolectadas en el campo en ganado cebú (Bos indicus) y ganado exótico (Bos taurus) con niveles altos de anticuerpos contra antígeno de esquizontes deTheileria parva, transmitieron la infección conTh. parva, típica de Fiebre de la Costa Este, a ganado susceptible. Las ninfas no infectadas deR. appendiculatus, aplicadas a cebú recuperado de la infección y a ganado exótico mantenidos libres de garrapatas en el laboratorio durante 16 y 7 meses respectivamente, transmitieron theileriosis fatal a ganado susceptible. El ganado inmunizado por infección y tratamiento posterior fueron portadores deTheileria parva mediante examen de las glándulas salivares de garrapatas nuevamente aplicadas a dicho ganado y mediante transmisión vectorial. Se pudieron establecerin vitro linfocitos infectados porTheileria parva, provenientes de la sangre periférica 3 y 7 meses después de la inmunización. Esta ha sido la primera demonstraciónin vitro de la existencia de esquizontes en el estado de portador en infecciones conTheileria parva.

Este estudio demuestra que el ganado en áreas endémicas y epidémicas, permanece como portador, manteniendo asi la población deTh. parva. Se discute la importancia de este hallazgo, para el control de la Fiebre de la Costa Este mediante inmersión, inmunización y tratamiento.

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Kariuki, D.P., Young, A.S., Morzaria, S.P. et al. Theileria parva carrier state in naturally infected and artificially immunised cattle. Trop Anim Health Prod 27, 15–25 (1995). https://doi.org/10.1007/BF02236328

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Keywords

  • Salivary Gland
  • Como
  • Peripheral Blood Lymphocyte
  • Carrier State
  • Mese