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Theileria parva carrier state in naturally infected and artificially immunised cattle

L'etat De Porteur DeTheileria parva Chez Des Bovins Naturellement Infectes Et Immunises Artificiellement

El Estado De Portador DeTheileria Parva En Animales Infectados En Forma Natural Y Artificialmente Mediante Inmunizacion


Rhipicephalus appendiculatus adult ticks, collected in the field from zebu (Bos indicus) and exotic (Bos tarus) cattle with high antibody titres toTheileria parva schizont antigen, transmittedTheileria parva infection typical of East Coast fever to susceptible cattle. UninfectedR. appendiculatus nymphs applied to naturally recovered zebu and exotic cattle kept under tick-free conditions in the laboratory for 16 and 7 months respectively, transmitted fatal theileriosis to susceptible cattle. Cattle immunised by the infection and treatment method were shown to be carriers ofTheileria parva by examination of the salivary glands of ticks applied to them and by tick transmission. Three and 7 months after immunisation,Theileria parva infected lymphocytes were establishedin vitro from peripheral blood lymphocytes. This was the first demonstration,in vitro, of the existence of schizonts inTheileria parva (East Coast fever) infection carrier status.

These studies show that cattle from endemic and epidemic areas of East Coast fever (ECF) become carriers thereby maintaining theT. parva population. The relevance of the findings in this study to the control of ECF by dipping, immunisation and treatment is discussed.


Des tiques adultes deRhipicephalus appendiculatus récoltées sur des zébus (Bos indicus) et des bovins importés (Bos taurus) présentant des taux élevés d'anticorps vis à vis d'antigènes de schizontes deTheileria parva, ont transmis une infection àTh. parva typique de theileriose à des bovins sensibles. Des nymphes deR. appendiculatus non infectées, appliquées sur des zébus naturellement guéris et placées ultérieurement sur des bovins importés maintenus à l'abri des tiques pendant 16 et 17 mois respectivement on transmis une theileriose mortelle aux bovins sensibles. Les bovins immunisés par la méthode infection et traitement se sont révélés porteurs deTh. parva par l'examen des glandes salivaires de tiques fixées sur eux et par la transmission par les tiques, et 3 et 7 mois après immunisation des lymphocytes infectés parTh. parva ont été mis en évidencein vitro à partir des lymphocytes du sang periphérique. Il s'agit de la première observation,in vitro, de l'existence de schizontes chez des animaux porteurs de theileriose àTh. parva. Ces études montrent que les bovins des régions où sévit la theileriose à l'état épidémique où endémique deviennent porteurs, maintenant ainsi la population deTh. parva. Les observations lors de cette étude concernant la lutte contre la theileriose par l'utilisation de bains détiqueurs, l'immunisation et le traitement sont discutées.


Garrapatas adultasRhipicephalus appendiculatus, recolectadas en el campo en ganado cebú (Bos indicus) y ganado exótico (Bos taurus) con niveles altos de anticuerpos contra antígeno de esquizontes deTheileria parva, transmitieron la infección conTh. parva, típica de Fiebre de la Costa Este, a ganado susceptible. Las ninfas no infectadas deR. appendiculatus, aplicadas a cebú recuperado de la infección y a ganado exótico mantenidos libres de garrapatas en el laboratorio durante 16 y 7 meses respectivamente, transmitieron theileriosis fatal a ganado susceptible. El ganado inmunizado por infección y tratamiento posterior fueron portadores deTheileria parva mediante examen de las glándulas salivares de garrapatas nuevamente aplicadas a dicho ganado y mediante transmisión vectorial. Se pudieron establecerin vitro linfocitos infectados porTheileria parva, provenientes de la sangre periférica 3 y 7 meses después de la inmunización. Esta ha sido la primera demonstraciónin vitro de la existencia de esquizontes en el estado de portador en infecciones conTheileria parva.

Este estudio demuestra que el ganado en áreas endémicas y epidémicas, permanece como portador, manteniendo asi la población deTh. parva. Se discute la importancia de este hallazgo, para el control de la Fiebre de la Costa Este mediante inmersión, inmunización y tratamiento.

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  1. Bailey, K. P. (1960). Notes on the rearing ofRhipicephalus appendiculatus and the infection withTheileria parva for experimental transmission.Bulletin of Epizootic Diseases of Africa,8, 33–64.

  2. Barnett S. F. (1968).Theileriosis In:Infectious Blood Diseases of Man and Animals. Vol. II. (Ed. D. Weinman and M. Ristic). Academic Press, New York, pp 269–328.

  3. Barnett, S. F. &Brocklesby, D. W. (1966). Recent investigation of Theileridae of cattle and buffalo in Africa. A mild form of East Coast fever (Theileria parva) with persistence of infection.British Veterinary Journal,122, 362–370.

  4. Bevan, L. E. W. (1924). East Coast fever—the theory of latency.Transactions of the Royal Society Tropical Medicine Hygiene.18, 328–336.

  5. Brown, C. G. D. (1979). Propagation ofTheileria. In:Practical Tissue Culture Application. (Eds K. Maramorosch and H. Hirumi), Academic Press, New York. pp 223–245.

  6. Burridge, M. J. &Kimber, C. D. (1972). The indirect fluorescent antibody test for experimental East Coast fever (Theileria parva) infection in cattle: Evaluation of cell culture schizont antigen.Research in Veterinary Science,13, 451–455.

  7. Conrad, P. A., Denhan, D. &Brown, C. G. D. (1986). Intraerythrocytic multiplication ofTheileria parva in vitro: an ultrastructure study.International Journal of Parasitology,16, 223–230.

  8. Conrad, P. A., Kelly, B. G. &Brown, C. G. D. (1985). Intraerythrocytic schizogony ofTheileria annulata.Parasitology,91, 67–82.

  9. Dolan, T. T. (1981). Progress in the chemotherapy of theileriosis. In:Advances in the Control of Theileriosis. (Eds A. D. Irvin, M. P. Cunningham and A. S. Young). Martinus Nijhoff, The Hague, pp 186–208.

  10. Dolan, T. T. (1986). Chemotherapy of East Coast fever: the long term weight changes, carrier state and disease manifestation of parvaquone treated cattle.Journal of Comparative Pathology,96, 137–146.

  11. du Toit, P. J. (1928). Observation on immunity in East Coast fever.South Africa Journal of Science,25, 282–287.

  12. du Toit, P. J. (1931). Immunity in East Coast fever. 17th Report of the Director of Veterinary Services and Animal Industry. Pretoria, pp 3–25.

  13. FAO, (1984).Tick and Tick-borne disease control: A practical field mannual. Vol. I and II. Food and Agricultural organisation, Rome, 621 pp.

  14. Henning, M. W. (1949).Animal Diseases in South Africa. 2nd ed. Central News Agency Ltd, Durban, pp 415–417.

  15. Irvin, A. D. (1987). Characterization of species and strains ofTheileria.Advances in Parasitology,26, 145–197.

  16. Irvin, A. D., Dobbelaere, D. A. E. Mwamachi, D. M., Minami, T., Spooner, P. R. &Ocama, J. G. R. (1983). Immunization against East Coast fever: Correlation between monoclonal antibody profiles ofTheileria parva stocks and cross immunityin vivo.Research in Veterinary Science,5, 341–345.

  17. Lounsbury, C. P. (1904). Transmission of East Coast fever.Agricultural Journal Cape Province,24, 428–430.

  18. Mettam, R. W. &Carmichael, J. (1936). Turning sickness, a protozoa encephalitis of cattle in Uganda. Its relationship with East Coast fever.Parasitology,28, 254–283.

  19. Minami, T., Spooner, P. R., Irvin, A. D., Ocama, J. G. R., Dobbelaere, D. A. E. &Fujinaga, T. (1983). Characterization ofT. parva stocks by monoclonal antibody profiles.Research in Veterinary Science,35, 334–340.

  20. Morzaria, S. P., Irvin, A. D., Taracha, E. L. N., Spooner, P. R., Voigt, W. P., Fuginaga, T. &Katende, J. K. (1987). Immunization against East Coast fever: the use of selected stocks ofTheilera parva for immunization of cattle exposed to field challenge.Veterinary Parasitology,23, 23–41.

  21. Mukhebi, A. W., Perry, B. D. &Kruska, R. (1992). Estimated economics of theileriosis control in Africa.Preventive Veterinary Medicine,12, 73–85.

  22. Mutugi, J. J., Young, A. S., Maritim, A. C., Mining, S. K., Linyonyi, A., Ngumi, P. N., Leitch, B. L., Morzaria, S. P. &Dolan, T. T. (1989). Immunization against Theileriosis in Coast Province, Kenya: Laboratory evaluation of a largeTheileria parva stabilate for use in infection and treatment immunization in the field.Research in Veterinary Science,47, 170–179.

  23. Mutugi, J. J., Young, A. S., Kariuki, D. P., Morzaria, S. P. &Ole Tameno, J. M. (1991) Epidemiological observations on theileriosis following field immunization using infection and treatment with either oxytetracycline and buparvaquone.Tropical Animal Health and Production,23, 75–82.

  24. Nietz, W. O. (1957). Theileriosis, gonderiosis and cytauxzoonoses: A review.Onderstepoort Journal of Veterinary Research,27, 275–430.

  25. Radley, D. E. (1981). Infection and treatment method of immunization against Theileriosis. In:Advances in the Control of Theileriosis. (Ed. A. D. Irvin, M. P. Cunningham and A. S. Young) Martinus Nijhoff, The Hague, pp 227–237.

  26. Radley, D. E., Brown, C. G. D., Cunningham, M. P., Kimber, C. D., Musisi, F. C., Payne, R. C., Purnell, R. E., Staff, D. A. &Young, A. S. (1975). East Coast fever: Chemoprophylaxis immunization of cattle using oxytetracycline and a combination ofTheileria strains.Veterinary Parasitology,1, 51–60.

  27. Stagg, D. A., Brown, C. G. D., Crawford, J. G., Kanhai, G. K. &Young, A. S. (1974).In vitro cultivation ofTheileria lawrencei—infected lymphoblastoid cell lines derived from a buffalo (Syncerus caffer).Research in Veterinary Science,16, 125–127.

  28. Theiler, A. (1903). The Rhodesian tick fever.Transvaal Agricultural Journal,1, 37–41.

  29. Theiler, A. (1905). Transmission of East Coast fever by ticks. Report of the Government Veterinary Bacteriologist, Transvaal, 1903–1904, pp 135–150.

  30. Theiler, A. (1906). Piroplasm mutans (n. spec.) of South African cattle.Journal of Comparative Pathology and Therapeutics,19, 292–300.

  31. Theiler, A. (1908). Further transmission experiments with East Coast fever. Report of Government Veterinary Bacteriologist, Transvaal, 1906–1907, pp 70–85.

  32. Uilenburg, G. (1983). Tick-borne livestock diseases and their vectors. 2. Epizootiology of tick-borne diseases.World Animal Health Review,8, 8–19.

  33. Wilde, J. K. H. (1967). East Coast fever.Advances in Veterinary Science,1, 207–259.

  34. Young, A. S., Brown, C. G. D., Burridge, M. J., Grootenhuis, G. J., Kanhai, G. K., Purnell, R. E. &Stagg, D. A. (1978). Incidence of theilerial parasites in the East African buffalo (Syncerus caffer).Tropenmedizin und parasitologie,29, 281–288.

  35. Young, A. S., Groocock, C. M. &Kariuki, D. P. (1988). Integrated control of ticks and tick-borne diseases of cattle in Africa.Parasitology,96, 403–432.

  36. Young, A. S. &Leitch, B. L. (1981a). Epidemiology of East Coast fever: Some effects of temperature on the development ofTheileria parva in the tick vector,Rhipicephalus appendiculatus.Parasitology,83, 199–211.

  37. Young, A. S., Leitch, B. L. &Newson, R. M. (1981b). The occurrence ofT. parva carrier state in cattle from an East Coast fever endemic area of Kenya. In:Advances in the Control of Theileriosis. (Eds. A. D. Irvin, M. P. Cunningham and A. S. Young). Martinus Nijhoff Publishers. The Hague, pp 60–62.

  38. Young, A. S., Leitch, B. L., Newson, R. M. &Cunningham, M. P. (1986). Maintenance ofTheileria parva parva in an endemic area of Kenya.Parasitology,93, 9–16.

  39. Young, A. S. &Newson, R. M. (1974). An improved method of handling ticks collected in the field.Research in Veterinary Science,37, 133–135.

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Kariuki, D.P., Young, A.S., Morzaria, S.P. et al. Theileria parva carrier state in naturally infected and artificially immunised cattle. Trop Anim Health Prod 27, 15–25 (1995). https://doi.org/10.1007/BF02236328

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  • Salivary Gland
  • Como
  • Peripheral Blood Lymphocyte
  • Carrier State
  • Mese