An inhibitor of gastric-acid secretion has been purified from human saliva and its activity concentrated 30-fold. Inactive material has been removed from saliva by extraction into phenol, by digestion with protease, by heat precipitation, and by prolonged dialysis. The final preparation contained polysaccharide or mucopolysaccharide of molecular weight in excess of 100,000, as well as traces of sialic acid and protein.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Code, C. F. Discussion of paper by Smith, W. O., Hoke, R., Landy, J., Caputto, R., and Wolf, S. Nature of the inhibitory effect of normal human gastric juice on Heidenhain pouch dogs.Gastroenterology 34:181, 1958.
Brunschwig, A., Prohaska, J. V., Clarke, T. H., andKandel, E. V. A secretory depressant in gastric juice of patients with pernicious anaemia.J Clin Invest 18:415, 1939.
Livermore, G. R., andCode, C. F. A possible gastric secretory inhibitor in canine gastric juice.Amer J Physiol 168: 605, 1952.
Code, C. F., Ratke, H. V., Livermore, G. R., andLundberg, W. Occurrence of gastric secretory inhibitor activity in fresh gastric and salivary mucin (abstract).Fed Proc 8: 26, 1949.
Code, C. F., Blackburn, C. M., Livermore, G. R., andRatke, H. V. A method for quantitative determination of gastric secretory inhibition.Gastroenterology 13:573, 1949.
Menguy, R., Masters, Y. F., andGryboski, W. A. Studies on the origin of the gastric inhibitory substance in gastric juice.Surgery 58:535, 1965.
Rudick, J., Gajewski, A. K., Pitts, C. L., Semb, L. S., Fletcher, T. L., Harkins, H. N., andNyhus, L. M. Gastric inhibitors in fasting canine thoracic duct lymph.Proc Soc Exp Biol 120:119, 1965.
Glass, G. B. J. The natural history of gastric atrophy: A review of immunologic aspects and possible links to endogenous inhibitors of gastric secretion.Amer J Dig Dis 10:376, 1965.
Morgan, W. T., andKing, H. K. Studies in immunochemistry (7): The isolation from hog gastric mucin of the polysaccharide-amino acid complex possessing blood group A activity.Biochem J 37:640, 1943.
Morgan, W. T. J. Some observations on the carbohydrate containing components of human ovarian cyst mucin.Ann NY Acad Sci 106:177, 1963.
Shay, H., Sun, D. C. H., andGruenstein, M. A quantitative method for measuring spontaneous gastric secretion in the rat.Gastroenterology 26:906, 1954.
Menguy, R., andSmith, W. O. Inhibition of gastric secretion in the rat by normal human gastric juice.Proc Soc Exp Biol 102:665, 1959.
Menguy, R., Masters, Y., andGryboski, W. Content of gastric inhibitory substances in saliva of patients with various gastric disorders.Gastroenterology 46:32, 1964.
Katzka, I. A gastric secretory inhibitor in normal and pernicious anaemia.Gastroenterology 43:71, 1964.
Rowe, P. B., Fenton, B. H., andBeeston, D. Further observations on the gastric secretory depressant in human gastric juice.Gut 5:324, 1964.
Baume, P. E., Nicholls, A., Barnett, R. A., andRoom, R. A. Rat gastric acid secretion in response to graded subcutaneous doses of 2-deoxy-D-glucose.Amer J Physiol 211: 626, 1966.
Dixon, W. J., and Massey, F. J.Introduction to Statistical Analysis ed. 2, McGraw-Hill, New York, 1957, p. 290.
Gornall, A. G., Bardawill, C. J., andDavid, M. M. Determination of serum proteins by means of Biuret reaction.J Biol Chem 177:751, 1949.
Morris, D. L. Quantitative determination of carbohydrates with Dreywood's anthrone reagent.Science 107:254, 1948.
Richmond, V., Caputto, R., andWolf, S. Fractionation of the non-dialyzable soluble components of gastric contents by chromatography on Amberlite IRC-50.Arch Biochem 66:155, 1957.
Warren, L. The thiobarbituric assay of sialic acids.J Biol Chem 234:1971, 1959.
Judd, J., Clouse, W., Ford, J., van Eys, J., andCunnincham, L. W. An automatic determination of protein-bound hexose.Anal Biochem 4:512, 1962.
Leach, S. A. Release and breakdown of sialic acid from human salivary mucin and its role in the formation of dental plaque.Nature (London) 199:486, 1963.
Leach, S. A. Some observations on the state of sialic acid in human saliva.Arch Oral Biol 9:461, 1964.
Mencuy, R., andBerlinski, M. Source of sialogastrone, a gastric inhibitory substance in human saliva.Amer J Dig Dis 12:1, 1967.
The authors wish to record their indebtedness to Drs. D. B. Morell, F. J. Radcliff, and J. Gagolski for their advice and encouragement with this study and to the Postgraduate Medical Foundation in the University of Sydney for generous financial support.
Research Fellow of the Royal Australasian College of Physicians.
About this article
Cite this article
Baume, P.E., Baxter, C.H. & Nicholls, A. Concentration and partial characterization of human salivary gastrone. Digest Dis Sci 12, 965–972 (1967). https://doi.org/10.1007/BF02233254
- Public Health
- Molecular Weight