Anti-SSA/Ro and anti-SSB/La antibodies in serum and saliva of patients with Sjogren's syndrome
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Summary
Autoantibodies to SSA/Ro and SSB/La antigens may have a pathogenic role in photosensitive skin disease and congenital complete heart block. Since salivary glands are the major target organ in Sjogren's syndrome (SS) we wondered whether these autoantibodies are present in saliva and may be involved in the sicca syndrome. Whole saliva and serum were collected from 15 patients with SS. Elisa analysis disclosed that 8 of the patients had anti-SSA/Ro antibodies, while 6 of them also had anti-SSB/La antibodies. Studies of immunoglobulin classes showed that the sera contained mainly IgG and IgM anti-SSA/Ro or SSB/La antibodies. One serum also contained IgA antibodies. Analysis of the saliva showed that in all positive samples IgG and IgA classes were present, while none of them contained IgM. Elisa and immunoblot analysis of sera and saliva from SLE patients without the sicca syndrome disclosed that both fluids contained anti-Sm antibodies. These findings suggest that the presence of anti-SSA/Ro and anti-SSB/La antibodies in saliva is not a unique phenomenon, characterizing the sicca syndrome. Therefore, their role in the pathogenicity of the Sjogren's syndrome has to be elucidated.
Key words
Sjogren's Syndrome SSA/Ro Antibodies SSB/La Antibodies SalivaPreview
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References
- 1.Tan, E.M. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 1989, 44, 93–151.PubMedGoogle Scholar
- 2.Harley, J.B., Alexander, E.L., Bias, W.B., Fox, O.F., Provost, T.T., Reichlin, M., Yamagata, H, Arnett, F.C. Anti-SSA (Ro) and anti-SSB(La) in patients with Sjogren's syndrome. Arthritis Rheum 1986, 196–206.Google Scholar
- 3.Ben-Chetrit, E., Fox, R.I., Tan, E.M. Dissociation of immune responses to the SS-A (Ro) 52 kD and 60 kD polypeptides in systemic lupus erythematosus and Sjogren's syndrome. Arthritis Rheum 1990, 33, 349–355.PubMedGoogle Scholar
- 4.Lee, L.A., Garther, K.K., Coulter, S.N., Norris, D.A., Harley, J.B. Pattern of cutaneous immunoglobulin G deposition in subacute cutaneous lupus erythematosus is reproduced by infusing purified anti-Ro (SSA) autoantibodies into human skin-grafted mice. J Clin Invest 1989, 83, 1556–1562.PubMedGoogle Scholar
- 5.Davis, T.L., Lyde, C.B., Davis, B.M., Sontheimer, R.D. Perturbation of experimental ultraviolet light induced erythema by passive transfer of serum from subacute cutaneous lupus erythematosus patients. J Invest Dermatol 1989, 92, 573–577.PubMedGoogle Scholar
- 6.Buyon, J.P., Ben-Chetrit, E., Karp, S., Roubey, R.A.S., Pompeo, L., Reeves, W.H., Tan, E.M., Winchester, R. Acquired congenital heart block: pattern of maternal antibody response to biochemically defined antigens of SSA/Ro-SSB/La system in neonatal lupus. J Clin Invest 1989, 84, 627–634.PubMedGoogle Scholar
- 7.Silverman, E., Mamula, M., Hardin, J.A., Laxter, R. Importance of immune response to the Ro/La particle in the development of congenital heart block and neonatal lupus erythematosus. J Rheumatol 1991, 18, 120–124.PubMedGoogle Scholar
- 8.Fox, I.R., Robinson, C.A., Curd, J.G., Kozin, F., Howell, F.V. Sjogren's syndrome: proposed criteria for classification. Arthritis Rheum 1986, 29, 577–585.PubMedGoogle Scholar
- 9.Towbin, H., Staehelin, T., Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 1979, 76, 4350–4354.PubMedGoogle Scholar
- 10.Horsfall, A.C., Rose, L.M., Maini, R.N. Autoantibody synthesis in salivary glands of Sjogren's syndrome patients. J Autoimmun 1989, 2, 559–568.PubMedGoogle Scholar
- 11.Charles, P.J., Foster, H., Shuttles, W., Griffith, I., Maini, R.N. Salivary antibodies to Ro and La in primary Sjogren's syndrome. PD3-50. Presentation at annual meeting of Eur Assoc Rheumatol, Budapest, 1991.Google Scholar
- 12.Elkon, K.B., Gharavi, A.E., Patel, B.M., Hughes, G.R., Frankel, A. IgA and IgM rheumatoid factors in serum, saliva and other secretions: relationship to immunoglobulin ratios in systemic sicca syndrome and rheumatoid arthritis. Clin Exp Immunol 1983, 52, 75–84.PubMedGoogle Scholar