Plant and Soil

, Volume 110, Issue 2, pp 269–274 | Cite as

Field inoculation of sorghum and rice withAzospirillum spp. andHerbaspirillum seropedicae

  • J. A. R. Pereira
  • V. A. Cavalcante
  • J. I. Baldani
  • Johanna Döbereiner


Two field experiments were carried out at the UAPNPBS experimental station, Seropédica, with two sorghum and one rice cultivars. The establishment, and inoculation effects, ofAzospirillum spp. andHerbaspirillum strains marked with antibiotic resistance were investigated. One grain sorghum (BR 300) and one sugar sorghum (Br 505) cultivar were used.Azospirillum lipoferum strain S82 (isolated from surface sterilized roots of sorghum) established in both cultivars and comprised 40 to 80% of theAzospirillum spp. population in roots and stems 60 days after plant emergence (DAE).Azospirillum amazonense strain AmS91 (isolated from surface-sterilized roots of sorghum) reached only 50%. At 90 DAE, S82 almost disappeared (less than 30% of establishment) while the establishment of AmS91 remained constant in roots and stems. No establishment ofH. seropedicae strain H25 (isolated from surface-sterilized roots of sorghum) orA. lipoferum strain S65 (isolated from the root surface of sorghum) could be observed on inoculated roots. Inoculation with S82, AmS91 or S65 but not withH. seropedicae H25, increased plant dry weight of both cultivars and total N in grain of the grain sorghum. In rice,A. lipoferum Al 121 andA. brasilense Sp 245 (isolated from surface sterilized rice and wheat roots respectively) established in the roots but there was no increase inAzospirillum spp. numbers due to inoculation. None of the strains affected plant growth or rice grain yield.Azospirillum amazonense, A82 andH. seropedicae Z95, which did not establish in roots, significantly enhanced seed germination.

Key words

antibiotic resistance Azospirillum inoculation establishment ofAzospirillum establishment ofHerbaspirillum Herbaspirillum inoculation rice sorghum 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • J. A. R. Pereira
    • 1
  • V. A. Cavalcante
    • 1
  • J. I. Baldani
    • 1
  • Johanna Döbereiner
    • 1
  1. 1.EMBRAPA-UAPNPBSSeropédicaBrazil

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