Plant and Soil

, Volume 110, Issue 2, pp 157–165 | Cite as

Mechanisms of infection of plants by nitrogen fixing organisms

  • J. I. Sprent
  • S. M. de Faria


Heterotrophic nitrogen-fixing microorganisms can enter plants via wounds, root hairs or intact epidermises. All at some stage need the ability to digest primary cell walls and/or middle lamellas. None appears to digest secondary walls. The ability of any organism to infect a particular plant reflects (a) the enzymes produced by the microorganism (and possibly, as part of its reaction, the plant); (b) the exact nature of the primary wall; (c) the distribution of secondary walls. Plants may respond to infection by hypersensitive and other reactions which could be triggered by production of cell wall fragments. Infection threads of secondary wall material may be essential for root hair infection and where cell boundaries are crossed. Entry into host cells other than by infection threads involves a delicate balance between endophyte and host. This may only be achieved in one or a few cells, which may then divide repeatedly to produce a symbiotic structure.

Key words

cell wall Frankia infection legume non-legume Rhizobium 


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  1. Barber S A and Silberbush M 1984 Plant root morphology and nutrient uptake.In Roots, Nutrient and Water Influx, and Plant Growth. Eds. S A Barber and D R Bouldin. pp. 65–87. ASA Special Publication No. 49. Soil Science Society of America, Madison.Google Scholar
  2. Batenburg F H D Van, Jonker R and Kijne J W 1986Rhizobium induces marked root hair curling by redirection of tip growth: A computer simulation. Physiol. Plant. 66, 476–480.Google Scholar
  3. Bender G L, Nayudu M, Goydych W and Rolfe B G 1988 Early infection events in the nodulation of the non-legumeParasponia andersonii byBradyrhizobium. Plant ScienceIn press Google Scholar
  4. Berry A M, McIntyre L and McCully M E 1986 Fine structure of root hair infection leading to nodulation in theFrankia-Alnus symbiosis. Can. J. Bot. 64, 292–305.Google Scholar
  5. Bradley D J, Butcher G W, Galfre G, Wood E A and Brewin N J 1986 Physical association between the peribacteroid membrane and lipopolysaccharide from the bacteroid outer membrane inRhizobium-infected pea root nodule cells. J. Cell Sci. 85, 47–61.PubMedGoogle Scholar
  6. Callaham D A and Torrey J G 1981 The structural basis for infection of root hairs inTrifolium repens byRhizobium. Can. J. Bot. 59, 1647–1664.Google Scholar
  7. Chandler M R 1978 Some observations on infection ofArachis hypogaea L. byRhizobium. J. Expt. Bot. 29, 749–755.Google Scholar
  8. Chandler M R, Date R A and Roughley R J 1982 Infection and root-nodule development inStylosanthes species byRhizobium. J. Expt. Bot. 33, 47–57.Google Scholar
  9. Clarkson D T, Robards A W, Stephens J E and Stark M 1987 Suberin lamellae in the hypodermis of maize (Zea mays) roots: Development and factors affecting the permeability of hypodermal layers. Plant. Cell Environ. 10, 83–93.Google Scholar
  10. Cutter E G 1969 Plant Anatomy: Experiment and Interpretation. Part I. Cells and Tissues. Edward Arnold, London.Google Scholar
  11. Dart P 1977 Infection and development of leguminous nodules.In A Treatise on Dinitrogen Fixation; Section III; Biology. Eds. R W F Hardy and W S Silver. pp. 367–472. Wiley Interscience, New York.Google Scholar
  12. Dixon R O D 1969 Rhizobia (with particular reference to relationships with host plants. Ann. Rev. Microbiol. 23, 137–158.Google Scholar
  13. Esau K 1965 Plant Anatomy, 2nd ed. Wiley, New York.Google Scholar
  14. Faria S M, McInroy S G and Sprent J I 1987 The occurrence of infected cells, with persistent infection threads, in legume root nodules. Can. J. Bot. 65, 553–558.Google Scholar
  15. Faria S M, Hay G T and Sprent J I 1988 Entry of rhizobia into roots ofMimosa scabrella Bentham occurs between epidermal cells. J. Gen. Microbiol. 134,In press.Google Scholar
  16. Foster R C 1981 The ultrastructure and histochemistry of the rhizosphere. New Phytol. 89, 263–273.Google Scholar
  17. Foster R C 1986 The ultrastructure of the rhizoplane and rhizosphere. Annu. Rev. Phytopath. 24, 211–234.Google Scholar
  18. Haahtela K, Laakso T and Korhonen T K 1986 Associative nitrogen fixation byKlebsiella spp.: Adhesion sites and inoculation effects on grass roots. Appl. Environ. Microbiol. 52, 1074–1079.Google Scholar
  19. Higashi S, Kushiyama K and Abe M 1987 Electron microscopic observations of infection threads in driselase treated nodules ofAstragalus sinicus. Can. J. Microbiol. 32, 947–952.Google Scholar
  20. Huang J-S 1986 Ultrastructure of bacterial penetration in plants. Annu. Rev. Phytopathol. 24, 141–157.Google Scholar
  21. Hubbell D H and Gaskins M H 1984 Associative N2 fixation withAzospirillum.In Biological Nitrogen Fixation—Ecology, Technology and Physiology. Ed. M Alexander. pp. 201–224. Plenum Press, New York.Google Scholar
  22. Justin S H F W and Armstrong W 1987 The anatomical characteristics of roots and plant response to soil flooding. New Phytol. 106, 465–495.Google Scholar
  23. Knowlton S and Dawson J O 1983 Effects ofPseudomonas cepacia and cultural factors on the nodulation ofAlnus rubra roots byFrankia. Can. J. Bot. 61, 2877–2882.Google Scholar
  24. Lancelle S A and Torrey J G 1984 Early development ofRhizobium-induced root nodules ofParasponia rigida. I. Infection and early nodule initiation. Protoplasma 123, 26–37.Google Scholar
  25. McNeil M, Darvill A G, Fry S C and Albersheim P 1984 Structure and function of the primary cell walls of plants. Annu. Rev. Biochem. 53, 625–663.PubMedGoogle Scholar
  26. Miller I M and Baker D D 1985 The initiation, development and structure of root nodules inElaeagnus angustifolia L. (Eleagnaceae). Protoplasma 128, 107–119.Google Scholar
  27. Miller I M and Baker D D 1986 Nodulation of actinorhizal plants byFrankia strains capable of both root hair infection and intercellular penetrations. Protoplasma 131, 82–91.Google Scholar
  28. Newcomb W 1981 Nodule morphogenesis and differentiation. Int. Rev. Cytol. Supplement 13, 247–298.Google Scholar
  29. Newcomb W and Pankhurst C E 1982a Fine structure of actinorhizal root nodules ofCoriaria arborea. N.Z. J. Bot. 20, 93–103.Google Scholar
  30. Newcomb W and Pankhurst C E 1982b Ultrastructure of actinorhizal root nodules ofDiscaria toumatou Raoul (Rhamnaceae). J. Bot. 20, 105–113.Google Scholar
  31. Okon Y and Kapulnik Y 1986 Development and function ofAzospirillum-inoculated roots. Plant and Soil 90, 3–16.Google Scholar
  32. Old K M and Nicholson T H 1978 The root cortex as part of a microbial continuum.In Microbial Ecology. Eds. M W Loutit and J A R Miles. pp. 291–294. Springer-Verlag, Berlin and Heidelberg.Google Scholar
  33. Patriquin D G, Döbereiner J and Jain D K 1983 Sites and processes of association between diazotrophs and grasses. Can. J. Microbiol. 29, 900–915.Google Scholar
  34. Smith C A, Skrvirsky R C and Hirsch A M 1986 Histochemical evidence for the presence of a suberin-like compound inRhizobium-like nodules of the nonlegumeParasponia rigida. Can. J. Bot. 64, 1474–1483.Google Scholar
  35. Sprent J I and McInroy S G 1984 Effects of salinity on growth and nodulation ofArachis hypogaea. In Advances in Nitrogen Fixation Research. Eds. C Veeger and W E Newton. p. 546. Martinus Nijhoff/Dr W Junk, Pudoc.Google Scholar
  36. Sprent J I and Raven J A 1985 Evolution of nitrogen-fixing symbioses. Proc. Roy. Soc. Edinburgh 85B, 215–237.Google Scholar
  37. Sprent J I, Sutherland J M and Faria, S. M. de 1987 Some aspects of the biology of nitrogen-fixing organisms. Phil. Trans. R. Soc. London B., 317, 119–121.Google Scholar
  38. Sprent J I, Sutherland J M and Faria S M de 1988 Structure and function of nodules from woody legumes.In Biology of Legumes. Eds. C H Stirton and J L Zarucchi. Missouri Botanic GardensIn press.Google Scholar
  39. Syono K, Newcomb W and Torrey J G 1976 Cytokinin production in relation to the development of pea root nodules. Can. J. Bot. 54, 2155–2162.Google Scholar
  40. Trinick M J 1982 Biology.In Nitrogen Fixation, Volume 2:Rhizobium. Ed. W J Broughton. pp. 76–146. Clarendon Press, Oxford.Google Scholar
  41. Turgeon B G and Bauer W D 1985 Ultrastructure of infection-thread development during the infection of soybean byRhizobium japonicum. Planta 163, 328–349.Google Scholar
  42. Umali-Garcia M, Hubbell D H and Gaskins M H 1978 Process of infection ofPanicum maximum bySpirillum lipoferum. Ecol. Bull. (Stockholm) 26, 373–379.Google Scholar
  43. Volkmann D 1984 The plasma membrane of growing root hairs is composed of zones of local differentiation. Planta 163, 392–403.Google Scholar
  44. Yahalom E, Okon Y and Dovrat A 1987Azospirillum effects on susceptibility toRhizobium nodulation and on nitrogen fixation of several forage legumes. Can. J. Microbiol. 33, 510–514.Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • J. I. Sprent
    • 1
  • S. M. de Faria
    • 1
  1. 1.Department of Biological SciencesUniversity of DundeeDundeeUK

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