Calcified Tissue Research

, Volume 24, Issue 1, pp 275–278 | Cite as

Localisation histochimique du calcium dans la colloïde des vésicules thyroïdiennes du rat

  • B. Dupuy
  • F. Rouais
  • P. Blanquet
Communication Brève
  • 18 Downloads

Résumé

Les auteurs utilisent une solution de Glyoxal bis 2-hydroxyanil à basse température pour localiser le calcium dans la thyroïde. Grâce à cette technique qui limite les mouvements ioniques, ils observent que le calcium situé principalement dans la colloïde est résorbé en même temps qu'elle lors d'une stimulation par le froid.

Mots clés

Calcium Thyroïde 

Histochemical localization of calcium in the colloid of rat thyroid vesicles

Summary

With a Glyoxal bis 2-hydroxyanil alcoholic solution at −25°C, the calcium was localized in frozen thyroid slices. The limited ionic movement permits seeing that the calcium situated principally in the colloid is resorbed at the same time by a low temperature stress.

Key words

Calcium Thyroid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliographie

  1. 1.
    Boland (de), A.R., Jilka, R.L., Martonosis, A.N.: Passive Ca2+ permeability of phospholipid vesicles and sarcoplasmic reticulum membranes. J. Biol. Chem.250, 7501–7510 (1975)PubMedGoogle Scholar
  2. 2.
    Borle, A.B.: Calcium transport in kidney cells and its regulation. In: Cellular mechanism for calcium transfer and homeostasis (G. Nichols and R.H. Wasserman, eds.) pp. 153–171. New York: Academic Press 1971Google Scholar
  3. 3.
    Clemente, F., Meldosi, J.: Calcium and pancreatic secretion. I. Subcellular distribution of calcium and magnesium in the exocrine pancreas of guinea pig. J. Cell. Biol.65, 88–102 (1975)PubMedGoogle Scholar
  4. 4.
    Dahl, G., Gratzl, M.: Calcium induced fusion of isolated secretory vesicles from the islets of Langerhans. Cytobiologie Zeitsch. für Exper. Zellf.12, 344–355 (1975).Google Scholar
  5. 5.
    Dumont, J.E., Boeynams, J.M., Brocas-Neuwels, H., Cantraine, F., Ketelbant-Balasse, P., Neve, P., Pochet, R., Rodesch, F., Swillens, S., Van Sande, J., Vassart, G.: Le métabolisme cellulaire de la thyroïde et sa régulation. Bull. Acad. Roy. Med. Belg.128, 819–839 (1973)Google Scholar
  6. 6.
    Dupuy, B., Rouais, F., Blanquet, P.: À propos de la localisation du calcium dans la thyroïde de rat. C.R. Soc. Biol. (sous presse) (1976)Google Scholar
  7. 7.
    Gabe, M.: In: Techniques histologiques, p. 39 Paris. Masson Ed. (1968).Google Scholar
  8. 8.
    Jobin, M., Ferland, L., Labrie, F.: Effect of pharmacological blockade of ACTH and TSH secretion on the acute stimulation of prolactin release by exposure to cold and ether stress. Endocr.99, 146 (1976)Google Scholar
  9. 9.
    Kajihara, A., Onaya, T., Yamada, T., Takemura, Y., Kotani, M.: Further studies on the acute stimulatory effect of cold on thyroid activity and its mechanism. Endocr.90, 538 (1972)Google Scholar
  10. 10.
    Kashiwa, H.K.: Calcium phosphate in osteogenic cells a critique of the glyoxal-bis-(2 hydroxyanil) and the dilute silver acetate methods. Clin. Orthop.70, 200–211 (1970)PubMedGoogle Scholar
  11. 11.
    Lupulescu, A., Petrovici, A.: In: Ultrastructure of the thyroid gland, p. 55. Basel: S. Karger 1968Google Scholar
  12. 12.
    Martoja, M.: Données histologiques et ultrastructurales sur le calcium thyroïdien. Calcif. Tiss. Res.14, 67–83 (1974)Google Scholar
  13. 13.
    Park, H.Z., Kashiwa, H.K.: Calcium localized in juxtanuclear granules of epiphyseal chondrocytes with a dilute glyoxal bis (2 hydroxyanil) solution. Calcif. Tiss. Res.19, 189–197 (1975)Google Scholar
  14. 14.
    Rasmussen, H., Goodman, D.B.P.: Calcium and c AMP as interrelated intracellular messengers. Ann. N.Y. Acad. Sci.253, 789–796 (1975)PubMedGoogle Scholar
  15. 15.
    Rose B., Loewenstein, W.R.: Calcium ion distribution in cytoplasm visualized by aequorin: diffusion in cytosol restricted by energized sequestering. Science190, 1204–1206 (1975)PubMedGoogle Scholar
  16. 16.
    Rouais, F., Dupuy, B., Laparra, J., Blanquet, P.: Étude quantitative du déplacement du calcium au cours de la fixation et de la déshydratation de la thyroïde de rat. J. of Microscopy (sous presse) (1976)Google Scholar
  17. 17.
    Simson, J.A.V., Spicer, S.S.: Selective subcellular localization of cations with variants of the potassium pyroantimonate technique. J. of histochem. Cytochem.23, 575–598 (1975)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • B. Dupuy
    • 1
  • F. Rouais
    • 1
  • P. Blanquet
    • 1
  1. 1.Inserm U.53Domaine de CarreireBordeauxFrance

Personalised recommendations