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Endoglucanase II (EGII) ofPenicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis

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The cDNA coding for the endoglucanase EGII ofP. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with theT. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. Theegl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcription startpoints were identified. Five motifs matching the binding site of the carbon-catabolite repressor CREA ofA. nidulans were detected. Their potential implication in repression was analyzed by bandshift assays.

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Correspondence to Günter Schulz.

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Communicated by A. Hinnen

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Mernitz, G., Koch, A., Henrissat, B. et al. Endoglucanase II (EGII) ofPenicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis. Curr Genet 29, 490–495 (1996).

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Key words

  • DNA-protein interactions
  • egl2 cDNA
  • egl2 promotor
  • Heterologous expression