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Current Genetics

, Volume 29, Issue 5, pp 446–456 | Cite as

The 3-phosphoglycerate kinase gene of the yeastYarrowia lipolytica de-represses on gluconeogenic substrates

  • M. -T. Le Dall
  • J. -M. Nicaud
  • B. Y. Tréton
  • C. M. Gaillardin
Original Paper

Abstract

We have isolated the 3-phosphoglycerate kinase (PGK) gene of the yeastYarrowia lipolytica by probing a genomic library with a PCR fragment amplified with primers deduced from two highly conserved regions of various PGKs. It is a unique sequence encoding a polypeptide of 417 residues with extensive homology to other PGKs, especially to that ofAspergillus nidulans (76% identity). The expression of theY. lipolytica PGK1 gene proved to be higher on gluconeogenic substrates than on glycolytic ones. Haploid strains harboring a disrupted allele were able to grow on mixtures of a gluconeogenic carbon source and of a glycolytic one, but required proline supplementation in the presence of glucose, and were inhibited by glycerol.

Key words

Yarrowia lipolytica 3-Phosphoglycerate kinase Expression Disruption 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. -T. Le Dall
    • 1
  • J. -M. Nicaud
    • 1
  • B. Y. Tréton
    • 1
  • C. M. Gaillardin
    • 1
  1. 1.Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, CBAIInstitut National AgronomiqueThiverval-GrignonFrance

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