Lead and PCB's in canvasback ducks: Relationship between enzyme levels and residues in blood
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Blood samples were taken for two successive years from canvasback ducks trapped in the Chesapeake Bay. The first winter (1972–1973) five plasma enzymes known to respond to organochlorine poisoning were examined. Abnormal enzyme elevations suggested that 20% of the population sampled (23/115 ducks) might contain organochlorine contaminants, but no residue analyses were performed. The second winter (1974) two of the same enzymes, aspartate aminotransferase and lactate dehydrogenase, and a third enzyme known to be specifically inhibited by lead, delta-aminolevulinic acid dehydratase, were assayed in 95 blood samples. Blood residues of organochlorine compounds and of lead were determined in representative samples, and the correlations between residue levels and enzyme changes were examined.
The enzyme bioassays in 1974 indicated that lead was a more prevalent environmental contaminant than organochlorine compounds in canvasback ducks; 17% of the blood samples had less than one-half of the normal delta-aminolevulinic acid dehydratase activity, but only 11% exhibited abnormal aspartate aminotransferase or lactate dehydrogenase activities. These findings were confirmed by residue analyses that demonstrated lead concentrations four times higher than background levels, but only relatively low organochlorine concentrations. There was a highly significant inverse correlation between delta-aminolevulinic acid dehydratase activity and blood lead concentrations (P<0.01), and a weaker but significant correlation between plasma aspartate aminotransferase activity and blood PCB concentrations (P<0.05).
It was apparent that delta-aminolevulinic acid dehydratase activity in the blood provided a sensitive and precise estimate of lead contamination in waterfowl. In canvasback ducks 200 ppb of lead in the blood caused a 75% decrease in delta-aminolevulinic acid dehydratase activity, a magnitude of enzyme inhibition that disturbs heme synthesis and is regarded as detrimental in humans.
KeywordsAspartate Aminotransferase Lead Concentration Residue Analysis Organochlorine Compound Lactate Dehydrogenase Activity
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- Allen, R. L.: The properties and biosynthesis of the haemoglobins. In Physiology and Biochemistry of the Domestic Fowl, pp. 873–881. Academic Press, New York (1971).Google Scholar
- Bellrose, F. C.: Lead poisoning as a mortality factor in waterfowl populations. Ill. Nat. Hist. Survey Bull.27, 235 (1959).Google Scholar
- Brace, K. and P. D. Altland: Life span of the duck and chicken erythrocyte as determined with C14. Proc. Soc. Exptl. Biol. Med.92, 615 (1956).Google Scholar
- Carney, S. M.: Preliminary keys to waterfowl age and sex identification by means of wing plumage.U. S. Fish Wildl. Serv. Spec. Rep. Wildl. No. 82 (1964).Google Scholar
- Committee on Biologic Effects of Atmospheric Pollutants: In Lead Airborne Lead in Perspective, pp. 205–212.National Academy of Sciences, Washington, D. C. (1972).Google Scholar
- Cromartie, E., W. L. Reichel, L. N. Locke, A. A. Belisle, T. Earl Kaiser, T. G. Lamont, B. M. Mulhern, R. M. Prouty, and D. M. Swineford: Residues of organochlorine pesticides and polychlorinated biphenyls and autopsy data for bald eagles, 1971 and 1972. Pestic, Monit. J.9, 11 (1975).Google Scholar
- Finley, M. T., M. P. Dieter, and L. N. Locke: Delta-aminolevulinic acid dehydratase: Inhibition in ducks dosed with lead shot. Environ. Res. In Press (1976).Google Scholar
- Grubbs, E. F.: Sample criterion for testing outlying observations. In Annals of Mathematical Statistics, Vol. 21, pp. 27–58 (1950).Google Scholar
- Hochbaum, H. A.: Sex and age determination of waterfowl by cloacal examination. Trans. 7th N. A. Wildlife Conf., Washington, D. C., pp. 209–307 (1942).Google Scholar
- Locke, L. N., G. E. Bagley, and H. D. Irby: Acid-fast intranuclear inclusion bodies in the kidneys of mallards fed lead shot. Bull. Wildl. Dis. Assoc.2, 127 (1966).Google Scholar
- Locke, L. N., and G. E. Bagley: Lead poisoning in a black duck. Bull. Wildl. Dis. Assoc.3, 37 (1967).Google Scholar
- Locke, L. N., H. D. Irby, and G. E. Bagley: Histopathology of mallards dosed with lead and selected substitute shot. Bull. Wildl. Dis. Assoc.3, 143 (1967).Google Scholar
- Stendell, R. C.: Personal communication.Google Scholar
- Tocci, P. M., J. B. Mann, J. E. Davies, and W. F. Edmundson. Biochemical differences found in persons chronically exposed to high levels of pesticides. Industr. Med.38, 40 (1969).Google Scholar
- U. S. Fish and Wildlife Service: Waterfowl Status Report.U. S. Fish Wildl. Serv. Spec. Sci. Rep. Wildl. No. 166 (1972).Google Scholar