Antonie van Leeuwenhoek

, Volume 37, Issue 1, pp 125–136 | Cite as

Bipolar budding in yeasts — an electron microscope study

  • N. J. W. Kreger-van Rij
  • M. Veenhuis
Article

Abstract

Bud formation in yeasts with bipolar budding was studied by electron microscopy of thin sections.

Budding in yeasts of the speciesSaccharomycodes ludwigii, Hanseniaspora valbyensis andWickerhamia fluorescens resulted in concentric rings of scar ridges on the wall of the mother cell. The wall between the ridges consisted of the scar plug left by the former budding and opened up in the formation of the next bud. The wall of the bud arose from under the wall of the mother cell.

In the yeasts of the speciesNadsonia elongata more than one bud might be formed from the same plug.

InSchizoblastosporion starkeyi-henricii the scar ridges were close together and apparently not separated by the entire plug.

In all species a cross wall was formed between mother cell and bud which consisted of an electron-light layer between two layers of more electron-dense material. The cells separated along the light layer.

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References

  1. Kreger-van Rij, N. J. W. andVeenhuis, M. 1969. A study of vegetative reproduction inEndomycopsis platypodis by electron microscopy. - J. Gen. Microbiol.58: 341–346.Google Scholar
  2. Nečas, O. andKopecká, M. 1969. Synthesis of the fibrillar component of regenerating cell walls in yeast protoplasts. - Antonie van Leeuwenhoek35, Suppl. B7–8.Google Scholar
  3. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. - J. Cell Biol.17: 208–212.CrossRefPubMedGoogle Scholar
  4. Sentandreu, R. andNorthcote, D. H. 1969. The formation of buds in yeast. - J. Gen. Microbiol.55: 393–398.PubMedGoogle Scholar
  5. Streiblová, E. 1968. Surface structure of yeast protoplasts. - J. Bacteriol.95: 700–707.PubMedGoogle Scholar
  6. Streiblová, E. andBeran, K. 1963. Types of multiplication scars in yeasts, demonstrated by fluorescence microscopy. - Folia Microbiol. (Prague)8: 221–227.Google Scholar
  7. Streiblová, E., Beran, K. andPokorný, V. 1964. Multiple scars, a new type of yeast scar in apiculate yeasts. - J. Bacteriol.88: 1104–1111.PubMedGoogle Scholar
  8. Vitols, E., North, R. J. andLinnane, A. W. 1961. Studies on the oxidative metabolism ofSaccharomyces cerevisiae. I. Observations on the fine structure of the yeast cell. - J. Biochem. Biophys. Cytol.9: 689–699.Google Scholar

Copyright information

© Swets & Zeitlinger 1971

Authors and Affiliations

  • N. J. W. Kreger-van Rij
    • 1
    • 2
  • M. Veenhuis
    • 1
    • 2
  1. 1.Laboratory of Bacteriology and SerologyState UniversityGroningenThe Netherlands
  2. 2.Laboratory of Ultrastructural BiologyState UniversityGroningenThe Netherlands

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