Bipolar budding in yeasts — an electron microscope study
Bud formation in yeasts with bipolar budding was studied by electron microscopy of thin sections.
Budding in yeasts of the speciesSaccharomycodes ludwigii, Hanseniaspora valbyensis andWickerhamia fluorescens resulted in concentric rings of scar ridges on the wall of the mother cell. The wall between the ridges consisted of the scar plug left by the former budding and opened up in the formation of the next bud. The wall of the bud arose from under the wall of the mother cell.
In the yeasts of the speciesNadsonia elongata more than one bud might be formed from the same plug.
InSchizoblastosporion starkeyi-henricii the scar ridges were close together and apparently not separated by the entire plug.
In all species a cross wall was formed between mother cell and bud which consisted of an electron-light layer between two layers of more electron-dense material. The cells separated along the light layer.
KeywordsElectron Microscope Thin Section Microscope Study Electron Microscope Study Mother Cell
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