Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Embryo growth ratein vitro as an indicator of embryo quality in IVF cycles

  • 47 Accesses

  • 23 Citations



The role of embryo growth rate in vitroas an isolated indicator of embryo quality in IVF was evaluated retrospectively.


Comparison was made between 98 cycles (group 1), in which only embryos at a cleavage stage of two to three blastomers were transferred, and 99 cycles (group 2), in which all embryos were transferred at a cleavage stage of four or more blastomers. Embryos were transferred at a fixed interval of 42 to 44 h following oocyte insemination. The mean patients' age, number of oocytes recovered and number of embryos transferred per cycle were similar in both groups.


The pregnancy rate and the take-home-baby rate were significantly higher in group 2 as compared to group 1 (22.2% vs 7.1% and 16.2% vs 4.1%; P <0.05 and P <0.01, respectively).


These results demonstrate that embryos with a slow cleavage rate in vitroare less likely to produce pregnancy following IVF-ET and that the cleavage stage is a valuable criteria in the selection of the best embryo for transfer.

This is a preview of subscription content, log in to check access.


  1. 1.

    Bolton VN, Hawes SM, Taylor CT, Parsons JH: Development of spare human preimplantation embryosin vitro: an analysis of the correlation among gross morphology, cleavage rates and development to blastocyst. Jin Vitro Fert Embryo Transfer 1989;6:30–35

  2. 2.

    Calman P, Randall Armant D, Seibel MM, Wang T, Oskowitz SP: The impact of embryo quality and quantity on implantation and the establishment of viable pregnancies. Jin Vitro Fert Embryo Transfer 1987;4:218–222

  3. 3.

    Cummins JM, Breen TM, Hanson KL, Shaw JM, Wilson LM, Henessey JF: A formula for scoring human embryo growth rates inin-vitro fertilization: its value in predicting pregnancy and in comparison with visual estimates of embryo quality. Jin Vitro Fert Embryo Transfer 1986;3:284–295

  4. 4.

    Edwards RG:In vitro fertilization and embryo replacement: opening lecture. Ann NY Acad Sci 1984;442:1–22

  5. 5.

    Puissant F, Rysselberge M, Barlow P, Deweze J, Leroy F: Embryo scoring as a prognostic tool in IVF treatment. Hum Reprod 1987;2:705–708

  6. 6.

    Trounson AO, Mohr LR, Wood C, Leeton JF: Effect of delayed insemination onin vitro fertilization, culture and transfer of human embryos. J Reprod Fertil 1982;64:285–294

  7. 7.

    Testart J: Cleavage stage of human embryos two days after fertilizationin vitro, and their developmental ability after transfer into the uterus. Hum Reprod 1986; 1:29–31

  8. 8.

    Lewin A, Laufer N, Rabinowitz R, Schenker JG: Ultrasonically guided oocyte recovery forin vitro fertilization An improved method. Jin Vitro Fertil Embryo Transfer 1986;3:370–373

  9. 9.

    Yanagimachi R: Zona-free hamster eggs: Their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa. Gamete Res 1984;10:187–232

  10. 10.

    Steer CV, Mills CL, Tan SL, Campbell S, Edwards RG: The cumulative embryo score: a predictive embryo scoring technique to select the optimal number of embryos to transfer in anin-vitro fertilization and embryo transfer programme. Hum Reprod 1992;7:117–119

  11. 11.

    Visser DS, Fourie FR: The application of the cumulative embryo score system for embryo selection and quality control in anin-vitro fertilization/embryo transfer programme. Hum Reprod 1993;8:1719–1722

Download references

Author information

Correspondence to Aby Lewin MD.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lewin, A., Schenker, J.G., Safran, A. et al. Embryo growth ratein vitro as an indicator of embryo quality in IVF cycles. J Assist Reprod Genet 11, 500–503 (1994). https://doi.org/10.1007/BF02216029

Download citation

Key words

  • cleavage
  • embryo
  • in vitro fertilization