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Rotavirus infections associated with diarrhoea in calves in Egypt

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The successful isolation and identification of rotavirus from newborn calves with diarrhoea is reported for the first time in Egypt. From 25 faecal samples taken from diarrhoeic calves, ten virus isolates were found to give cytopathogenic effects on bovine embryonic kidney cells. Three of the isolates were identified as rotavirus using fluorescent antibody staining, serum-neutralization, complement fixation and agar gel precipitation. The complement fixation test revealed the presence of rotavirus antibodies in 18 of 105 serum samples obtained from other calves slaughtered at Cairo abattoir.

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  1. Babiuk, L.A., Mohammed, K., Spence, L., Fauvel, M. and Petro, R. 1977. Rotavirus isolation and cultivation in the presence of trypsin. J. clin. Microbiol., 6: 610–617.

  2. Borden, E.C., Shope, R.E. and Murphy, F.A., 1971. Physico-chemical and morphological relationships of some arthropod-borne viruses to bluetongue virus. J. Gen. Virol., 13: 261–271.

  3. Feldman, H.A. and Wang, S.S., 1961. Sensitivity of various viruses to chloroform. Proc. Soc. exp. Biol., N.Y., 106: 736–738.

  4. Mebus, C.A., Kono, M., Underdahl, N.R. and Twiehaus, M. J., 1971. Cell culture propagation of neonatal calf diarrhea (scours) virus. Can. vet. J., 12: 69–71.

  5. Reed, L.J. and Muench, H., 1938. Simple method of estimating 50% end points. Am. J. Hyg., 27: 493–498.

  6. Scherrer, R., Cohen, J., L'Haridon, R., Feynerol, C. and Fayet, J.C., 1976. Reovirus-like agent (rotavirus) associated with neonatal calf gastroenteritis in France. Ann. Rech. vet., 7: 25–31.

  7. Stair, E.L., Rhodes, M.B., White, R.G., Mebus, C.A., 1972. Neonatal calf diarrhea: purification and electron microscopy of a coronavirus-like agent. Am. J. vet. Res., 33: 1147–1156.

  8. Thouless, M.E., Bryden, A.S., Flewett, T.H., Woode, G.N., Bridger, J.C., Snodgrass, D.R. and Herring, J.A., 1977. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch. Virol., 53: 287–294.

  9. Woode, G.N. and Bridger, J.C., 1975. Viral enteritis of calves. Vet. Rec., 96: 85–88.

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Shalaby, M.A., Saber, M.S. & El-Karamany, R.M. Rotavirus infections associated with diarrhoea in calves in Egypt. Vet Res Commun 5, 165–170 (1981). https://doi.org/10.1007/BF02214981

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  • Veterinary Medicine
  • Faecal Sample
  • Kidney Cell
  • Virus Isolate
  • Fixation Test