Veterinary Research Communications

, Volume 5, Issue 1, pp 101–115 | Cite as

Regulation of lactate metabolism in the rumen

  • G. H. M. Counotte
  • R. A. Prins
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Accepted:

Abstract

The regulation of lactic acid production, the regulation of lactate fermentation and the role of lactate as intermediate in the rumen metabolism was studied.

The pH had a pronounced effect on all three processes and therefore buffer capacity of the rumen contents is also described.

Starch gave much less rise to lactic acidosis than soluble sugars, as glucose and fructose. Most bacteria grow faster and therefore produce more lactic acid when amino acids and/or soluble proteins are present in the diet.

Activity of LDH (lactate dehydrogenase) of mixed rumen microorganisms is regulated by the NADH/NAD (H) balance and the ATP concentration. About 60% of the LDH in mixed rumen microorganisms is fructose-1, 6-diphosphate independent.

Megasphaera elsdenii ferments 60 to 80% of the lactate fermented in the rumen of dairy cattle.

Lactate accumulates only when the glycolytic flux (hexose units fermented per unit time per microorganism) is high. During adaptation, the glycolytic flux is increased and lactate may accumulate. After adaptation to a certain diet, the number of microorganisms is changed and the glycolytic flux again is normal and lactate is only a minor intermediate in rumen metabolism.

Keywords

Lactate Lactic Acid Fructose Lactate Dehydrogenase Hexose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Baldwin, R.L., Wood, W.A. and Emery, R.S., 1962. Conversion of lactate-C14 to propionate by the rumen microflora. J. Bacteriol. 83: 907–913.PubMedGoogle Scholar
  2. Bartley, E.E., 1976. Bovine saliva: production and function. In: M.S. Weinberg and A.L. Sheffner (Editor), Buffers in Ruminant Physiology and Metabolism. Church & Dwight Co. Inc. New York, pp. 61–81.Google Scholar
  3. Brüggemann, J. and Giesecke, D. 1968. Milchsäuregehalt in Pansen und mikrobieller Abbauin vitro. Zbl. Vet. Med. 15: 470–476.Google Scholar
  4. Byers, F.M. and Goodall, S.R., 1979. Effect of energy level on ruminal D(−) and L(+) lactic acid metabolism. J. Animal Sci. 48: 624–632.Google Scholar
  5. Counotte, G.H.M. and Prins, R.A., 1979. Regulation of rumen lactate metabolism and the role of lactic acid in nutritional disorders of ruminants. Vet. Sci. Commun. 2: 277–303.Google Scholar
  6. Counotte, G.H.M., van 't Klooster, A. Th. van der Kuilen, J. and Prins, R.A., 1979. An analysis of the buffer system in the rumen of dairy cattle. J. Animal Sci. 49: 1536–1544.Google Scholar
  7. Counotte, G.H.M. and Prins, R.A., 1979. Calculation of Km and Vmax from substrate concentration versus time plot. Appl. Environ. Microbiol. 38: 758–760.Google Scholar
  8. Counotte, G.H.M., de Groot, M. and Prins, R.A., 1980. Kinetic parameters of lactate dehydrogenase of some rumen bacterial species, the anaerobic rumen ciliateIsotricha prostoma and mixed rumen microorganisms. Antonie van Leeuwenhoek. 46: 363–381.PubMedGoogle Scholar
  9. Dehority, B.A. and Grubb, J.A., 1976. Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources. Appl. Environ. Microbiol. 32: 703–710.PubMedGoogle Scholar
  10. Doddema, H.J. and Vogels, G.D., 1978. Improved identification of methanogenic bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 36: 752–754.PubMedGoogle Scholar
  11. Hale, W.H. and Meinhardt, P. (ed.), 1979. Regulation of acid-base balance. Church & Dwight Co. Inc. New York.Google Scholar
  12. Hishinuma, F., Kanegasaki, S. and Takahashi, H., 1968. Ruminal fermentation and sugar concentrations. A model experiment withSelenomonas ruminantium. Agr. Biol. Chem. 32: 1325–1330.Google Scholar
  13. Huber, T.L., Cooley, J.H., Goetsch, D.D. and Das, N.K., 1976. Lactic acid utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ratio. Am. J. Vet. Res. 37: 611–613.PubMedGoogle Scholar
  14. Hungate, R.E., 1966. The rumen and its microbes. Academic Press Inc., New York and London.Google Scholar
  15. Huntington, G.B. and Britton, R.A., 1978. Effect of dietary lactic acid content and energy level on rumen lactate metabolism in sheep. J. Animal Sci. 47: 241–246.Google Scholar
  16. Huntington, G.B. and Britton, R.A., 1979. Effect of dietary lactic acid on rumen lactate metabolism and blood acid-base status of lambs switched from low to high concentrate diets. J. Animal Sci. 49: 1569–1576.Google Scholar
  17. Jayasuriya, G.C.N. and Hungate, R.E., 1959. Lactate conversions in the bovine rumen. Arch. Biochem. Biophys. 82: 274–287.PubMedGoogle Scholar
  18. Kay, R.N.B., 1966. The influence of saliva on digestion in ruminants. World. Rev. Nutr. Diet. 6: 292–325.PubMedGoogle Scholar
  19. Kunkle, W.E., Fetter, A.W. and Preston, R.L., 1976. Effect of diet onin vitro andin vivo rumen lactate disappearance in sheep. J. Animal Sci. 42: 1256–1262.Google Scholar
  20. Latham, M.J., Sharpe, E. and Sutton, J.D. 1971. The microbial flora of the rumen of cows fed hay and cereal rations and its relationship to the rumen fermentation. J. Appl. Bacteriol. 34: 425–434.PubMedGoogle Scholar
  21. Mackie, R.I. and Gilchrist, F.M.C., Robberts, A.M., Hannah, P.E. and Schwartz, H.M., 1978. Microbiological and chemical changes in the rumen during stepwise adaptation of sheep to high concentrate diets. J. Agric. Sci. 90: 241–254.Google Scholar
  22. Mackie, R.I. and Gilchrist, F.M.C., 1979. Changes in lactate-producing and lactate-utilizing bacteria in relation to pH in the rument of sheep during stepwise adaptation to a high-concentrate diet. Appl. Environ. Microbiol. 38: 422–430.Google Scholar
  23. Nakamura, K. and Takahashi, H., 1971. Role of lactate as an intermediate of fatty acid fermentation in the sheep rumen. J. Gen. Appl. Microbiol. 17: 319–328.Google Scholar
  24. Ogimoto, K. and Giesecke, D., 1974. Untersuchungen zur Genese und Biochemie der Pansenacidose. 2. Mikroorganismen und Umsetzung von Milchsäure-Isomeren. Zbl. Vet. Med. 21: 532–538.Google Scholar
  25. Ogimoto, K., 1977. Microbial investigation of lactic acid metabolic system in the rumen of feedlot cattles. Proc. Jap. Soc. Anim. Nutr. Metabol. 21: 74–86.Google Scholar
  26. Prins, R.A. and van der Meer, P., 1976. On the contribution of the acrylate pathway to the formation of propionate from lactate in the rumen of cattle. Antonie van Leeuwenhoek. 42: 25–31.PubMedGoogle Scholar
  27. Prins, R.A., 1977. Biochemical activities of gut microorganisms.In: R.T.J. Clarke and T. Bauchop (Editors), Microbial ecology of the gut. Academic Press, New York and London.Google Scholar
  28. Robinson, J.A. and Tiedje, J.M., 1980. Diurnal fluctuations of the H2 Pool in the bovine rumen. Abstr. Am. Soc. Microbiol. Meet., Miami, Florida, Abstr. I 123.Google Scholar
  29. Rogosa, M., 1964. The genusVeillonella. 1. General cultural, ecological and biochemical considerations. J. Bacteriol. 87: 162–170.PubMedGoogle Scholar
  30. Russel, J.B. and Baldwin, R.L., 1978. Substrate preferences in rumen bacteria: evidence of catabolite regulatory mechanisms. Appl. Environ. Microbiol. 36: 319–329.Google Scholar
  31. Russell, J.B. and Baldwin, R.L., 1979. Comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition. Appl. Environ. Microbiol. 37: 531–536.Google Scholar
  32. Ryan, R.K., 1964. Concentrations of glucose and low-mol-weight acids in the rumen of sheep changed gradually from a hay to a hay-plus-grain diet. Am. J. Vet. Res. 25: 653–659.PubMedGoogle Scholar
  33. Satter, L.D. and Esdale, W.J., 1968.In vitro lactate metabolism by ruminal ingesta. Appl. Microbiol. 16: 680–688.PubMedGoogle Scholar
  34. Stewart, C.S., 1972. Buffer capacity of nutrient media in relation to that of rumen fluid. Biochem. J. 127: 68.Google Scholar
  35. Stewart, C.S., 1975. Some effects of phosphate and volatile fatty acid salts on the growth of rumen bacteria. J. gen. Microbiol. 89: 319–326.Google Scholar
  36. Thauer, R.K., Jungermann, K. and Decker, K., 1977. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol. Rev. 41: 100–180.PubMedGoogle Scholar
  37. Thomas, T.D., Ellwood, D.C. and Longyear, V.M.C., 1979. Change from Homo- to Heterolactic fermentation byStreptococcus lactis resulting from glucose limitation in anaerobic chemostat cultures. J. Bacteriol. 138: 109–117.PubMedGoogle Scholar
  38. Turner, A.W. and Hodgetts, V.E., 1955. Buffer systems in the rumen of the sheep. II Buffering properties in relationship to composition. Australian J. Agric. Sci. 6: 125–144.Google Scholar
  39. Wallnöfer, P., Baldwin, R.L. and Stagno, E., 1966. Conversion of14C-labelled substrates to volatile fatty acids by the rumen microbiota. Appl. Microbiol. 14: 1004–1010.Google Scholar
  40. Weinberg, M.S. and Sheffner, A.L. (Editors), 1976. Buffers in ruminant physiology and metabolism. Church & Dwight Co. Inc. New York.Google Scholar

Copyright information

© Elsevier Scientific Publishing Company 1981

Authors and Affiliations

  • G. H. M. Counotte
    • 1
  • R. A. Prins
    • 1
  1. 1.Laboratory of Animal Nutrition, Zootechnical DepartmentState University of UtrechtUtrecht(The Netherlands)

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