Veterinary Research Communications

, Volume 6, Issue 1, pp 13–23 | Cite as

A reagin-like antibody in horse serum: 1. Occurrence and some biological properties

  • A. G. Matthews
  • P. Imlah
  • E. A. McPherson
Research Articles
Accepted:

Abstract

The demonstration of a reagin-like antibody againstCulicoides pulicaris extract in the serum of horses and ponies affected with recurrent seasonal dermatitis (sweet itch) is reported. This antibody can confer Prauznitz-Küstner (P−K) sensitivity on homologous skin for up to 5 days and, like human IgE, is thermolabile and susceptible to 2-mercaptoethanol reduction. It is eluted on diethylaminoethyl dextran-52 anion exchange chromatography independently of IgG, IgG(T) and IgM, and its elution characteristics indicate similarity in net molecular charge to human IgE.

The P−K response observed in horse skin is biphasic, and is morphologically similar to the late cutaneous anaphylactic response in man. Both phases of the P−K response are dependent upon the reagin-like antibody, although other serum factors appear involved in the delayed phase of the response.

Keywords

Public Health Dermatitis Veterinary Medicine Biological Property Horse Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Allen, P.Z. and Dalton, E.J., 1975. Studies on equine immunoglobulins. IV. Immunoglobulins of the donkey. Immunology, 28: 187–197.PubMedGoogle Scholar
  2. Augustin, R., 1967. Demonstration of reagins in the sera of allergic subjects. In: D.M. Weir (Editor), Handbook of Experimental Immunology. Blackwell Scientific Pub., Oxford and Edibburgh, pp. 1056–1151.Google Scholar
  3. Baker, K.P., 1978. The rational approach to the management of sweet itch. In: C.S. Grunsell and F.W.G. Hill (Editors), Veterinary Annual, 18th edition. Scientechnica, Bristol, pp. 163–168.Google Scholar
  4. Bennich, H., Johansson, S.G.O. and Bahr-Lindstrom, H., 1976. Immunolobulin E. In: E.B. Weiss and M.S. Segal (Editors), Bronchial Asthma: Mechanisms and Therapeutics. Little Braun and Company Inc., Boston, pp. 171–179.Google Scholar
  5. Bryant, J.H., Burns, M.W. and Lazarus, L., 1975. Identification of IgG antibody as a carrier of reaginic activity in asthmatic patients. J. Allergy clin. Immunol., 56: 417–428.PubMedGoogle Scholar
  6. Cordal, M.E. and Margni, R.A., 1974. Isolation, purification and biological properties of horse precipitating and non-precipitating antibodies. Immunochemistry, 11: 765–770.PubMedGoogle Scholar
  7. Devey, M.E., Wilson, D.V. and Wheeler, A.W., 1976. The IgG subclasses of antibodies to grass pollen allergens produced in hay fever patients during hyposensitization. Clin. Allergy, 6: 227–236.PubMedGoogle Scholar
  8. De Weck, A., 1972. In discussion of McGuire, T.C., Crawford, T.B. and Henson, J.B., 1972. The isolation, characterisation and functional properties of equine immunoglobulin classes and subclasses. Proc. 3rd Int. Conf. Equine Inf. Dis. Karger, Basel, pp. 364–381.Google Scholar
  9. Dolovich, J., Hargreaves, F.E., Chalmers, R., Shier, K.J., Gauldie, J. and Bienenstock, J., 1973. Late cutaneous allergic responses in isolated IgE dependent reactions. J. Allergy clin. Immunol., 52: 38–45.PubMedGoogle Scholar
  10. Dorrington, K.H. and Bennich, H., 1973. Thermally induced structural changes in immunoglobulin E. J. biol. Chem., 248: 8378–8384.PubMedGoogle Scholar
  11. Eyre, P., 1972. Anaphylactic (skin sensitising) antibodies in the horse. Vet. Rec., 90: 36–37.PubMedGoogle Scholar
  12. Fisher, J.P. and Connel, J.T., 1962. Passive cutaneous anaphylaxis in the guinea pig with serum of allergic patients treated with ragweed emulsions. J. Allergy, 33: 59–61.Google Scholar
  13. Halliwell, R.E.W., Fleischman, J.B., Mackay-Smith, M., Beech, J. and Gunson, D.E., 1979. The role of allergy in chronic pulmonary disease of horses. J. Am. vet. med. Assoc., 174: 277–281.PubMedGoogle Scholar
  14. Hirschfeld, J., 1960. Immunoelectrophoresis: procedure and application to the study of group specific variations in sera. Science Tools, 7: 18–25.Google Scholar
  15. Ishizaka, K. and Ishizaka, T., 1967. Identification of γE-antibodies as a carrier of reaginic activity. J. Immunol., 99: 1187–1198.PubMedGoogle Scholar
  16. Ishizaka, K. and Ishizaka, T., 1971 IgE immunoglobulins of human and monkey. In: E.L. Becker and K.F. Austen (Editors), Biochemistry of the Acute Allergic Reactions. Blackwell Scientific Pub., Oxford, pp. 13–30.Google Scholar
  17. Ishizaka, K. and Ishizaka, T., 1973. The role of IgE and IgG antibodies in reaginic hypersentivivity in the respiratory tract. In: K.F. Austen and L.M. Lichtenstein (Editors), Asthma. Academic Press, New York and London, pp. 55–68.Google Scholar
  18. Johansson, S.G.O., Bennich, H. and Wide, L., 1968. A new class of immunoglobulin in human serum. Immunology, 14: 265–272.PubMedGoogle Scholar
  19. Kuhns, W.J., 1961. Disappearance of diphtheria antitoxin from human passive transfer skin sites. Proc. Soc. exp. Biol. Med., 108: 63–67.PubMedGoogle Scholar
  20. Mansman, R.A. and Mansman, R.A.D., 1975. Equine immunology in health and disease. Am. Assoc. Equine Pract. Newsletter, 2: 44–72.Google Scholar
  21. McCaig, J. and Mellor, P.S., 1974. The probable cause of “sweet itch” in England. Vet. Rec., 95: 411–415.PubMedGoogle Scholar
  22. McPherson, E.A., Lawson, G.H.K., Murphy, J.R., Nicholson, Janet M., Breeze, R.G. and Pirie, H.M., 1979. Chronic obstructive pulmonary disease (COPD): Aetiological studies: responses to intradermal and inhalation antigen challenge. Equine vet. J., 11: 159–166.PubMedGoogle Scholar
  23. Neilsen, K.H., 1977. Bovine reaginic antibody. III. Cross reaction of anti-human IgE and antibovine reagin immunoglobulin with sera from several species of mammals. Can. J. comp. Med., 41: 345–348.PubMedGoogle Scholar
  24. Perelmutter, L. and Liakopoulou, A., 1971. Detection of IgE mediated immediate hypersensitivity reactions in the sera of ragweed sensitive individuals using rat mast cells. Int. Arch. Allergy, 40: 481–494.PubMedGoogle Scholar
  25. Raynaud, M. and Iskaki, S., 1970. Classification of equine immunoglobulins. Proceedings of the International Symposium on Antilymphocyte Serum, Versailles. Karger, Basel, pp. 101–124.Google Scholar
  26. Riek, R.F., 1954. Studies on allergic dermatitis (Queensland itch) of the horse: the aetiology of the disease. Aust. J. agric. Res., 5: 109–129.Google Scholar
  27. Ritzenthaler, M., 1924. L'anaphylaxie du cheval. Arch. int. Physiol., 24: 54–71.Google Scholar
  28. Schatzman, U., De Weck, A.L., Gerber, H., Lazary, S., Straub, R. and Pauli, B., 1973. Active and passive cutaneous anaphylaxis in the horse following immunisation with benzoyl-penicilloyl-bovine gammaglobulin (BPO20-BGG). Res. vet. Sci., 15: 347–352.PubMedGoogle Scholar
  29. Stanworth, D.R., 1973. Immediate hypersensitivity. North Holland Research Monographs, vol. 28. North Holland Publishing Company, Amsterdam.Google Scholar
  30. Stanworth, D.R. and Turner, M.W., 1973. Immunochemical analysis of immunoglobulins and their subunits. In: D.M. Weir (Editor). Handbook of Experimental Immunology, 2nd edition. Blackwell Scientific Pub., Oxford and Edinburgh, chapter 10.Google Scholar
  31. Umemoto, L., Poothullil, J., Dolovich, J. and Hargreave, F.E., 1976. Factors which influence late cutaneous allergic responses. J. Allergy clin. Immunol., 58: 60–68.PubMedGoogle Scholar
  32. Vaerman, J.P., 1970. Cited by Halliwell, R.E.W., Schwartzman, R.M. and Rockey, J.H., 1972. Antigenic relationship between human IgE and canine IgE. Clin. exp. Immunol., 10: 399–407.Google Scholar

Copyright information

© Elsevier Scientific Publishing Company 1983

Authors and Affiliations

  • A. G. Matthews
    • 1
  • P. Imlah
    • 2
  • E. A. McPherson
    • 1
  1. 1.Department of Veterinary MedicineUniversity of Edinburgh Veterinary Field StationRoslinUK
  2. 2.Department of Animal HealthUniversity of Edinburgh Veterinary Field StationRoslinUK

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