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The effect of α-amylase on the acrosomal membrane of human sperm

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Abstract

Purpose

Sperm capacitation and the acrosome reaction are prerequisites to accomplishing fertilization. The object of this research was to induce capacitation and the acrosome reaction of human sperm, comparing the acrosome status of fertile and infertile men, using α-amylase.

Methods

Acrosome status was measured using indirect immunofluorescence (IIF; monoclonal antibody GB24, Thèramex), hemizona assay (HZA), and transmission electron microscopy (TEM).

Results

Acrosome-reacted sperm without α-amylase treatment was significantly more abundant in the fertile versus the infertile group utilizing IIF, HZA, and TEM. α-Amylase-treated spermatozoa from the fertile and infertile groups showed a significant increase in the number of sperm bound to the hemizona (P <0.05).

Conclusions

The percentage of acrosome-reacted sperm in the infertile group was significantly enhanced by a-amylase, utilizing IIF and TEM.

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References

  1. 1.

    Overstreet JW: Transport of gametes in the reproductive tract of the female mammal.In Mechanism and Control of Animal Fertilization, JF Hartman (ed). New York, Academic Press, 1983, pp 499–543

  2. 2.

    Yudin AI, Hanson FW, Katz DF: Human cervical mucus and its interaction with sperm: A fine-structural view. Biol Reprod 1989;40:661–671

  3. 3.

    Vermeiden JPW, Bernardus RE, Ten Brug CS, Statema-Lohmeijer CH, Willemsen-Brugma AM, Schoemaker J: Pregnancy rate is significantly higher in vitro fertilization procedure with spermatozoa isolated from nonliquefying semen in which liquefaction is induced by α-amylase. Fertil Steril 1989;51:149–152

  4. 4.

    Calvo L, Dennison-Lagos L, Banks SM, Dorfman A, Thorsell LP, et al: Acrosome reaction inducibility predicts fertilization success at in-vitro fertilization. Hum Reprod 1994;9:1880–1886

  5. 5.

    Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, Van Zyl JA, Smith K: Sperm morphologic features as a prognostic factor in in-vitro fertilization. Fertil Steril 1986;46:1118–1123

  6. 6.

    Franken DR, Oehinger S, Burkman LJ, Coddington CC, Kruger TF, Rosenwaks Z, Acosta AA, Hodgen GD: The hemi-zona assay (HZA) A predictor of human sperm fertilizing potential in vitro fertilization (IVF) treatment. J Vitro Fert Embryo Transfer 1989;6:44–50

  7. 7.

    Franken DR, Burkman LJ, Oehinger SC, Coddington CC, Veeck LL, Kruger TF, Rosenwaks Z, Hodgen GD: Hemizona assay using salt-stored human oocytes: Evaluation of zona pellucida capacity for binding human spermatozoa. Gamete Res 1989;22:15–26

  8. 8.

    Edwards RG: Early human development: From the oocyte to implantation.In Scientific Foundations of Obstetrics and Gynaecology, Phillip EE, Barnes J, Newton M (eds). London, William Heinemann Medical Books, 1977, pp 175–252

  9. 9.

    Rose BI, Scott B: Sperm motility, morphology, hyperactivation, and ionophore-induced acrosome reactions after overnight incubation with mycoplasmas. Fertil Steril 1994;61:341–348

  10. 10.

    Kirton KT, Hafs HD: Sperm capacitation by uterine fluid or β-amylase in vitro. Science 1965;150:618

  11. 11.

    Skerlavay M, Epstein JA, Sobrero AJ: Cervical mucus amylase levels in normal menstrual cycles. Fertil Steril 1968;19:726

  12. 12.

    Tesarik J: Appropriate timing of the acrosome reaction is a major requirement for the fertilizing spermatozoon. Hum Reprod 1989;4:957–961

  13. 13.

    Tesarik J, Mendoza C: Sperm treatment with pentoxifylline improves the fertilizing ability in patients with acrosome reaction insufficiency. Fertil Steril 1993;60:141–148

  14. 14.

    Palermo G, Joris H, Devroey P, Van Steirteghem AC: Induction of acrosome reaction in human spermatozoa used for sub-zonal insemination. Hum Reprod 1992;57:1279–1288

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Correspondence to A. D. Esterhuizen.

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Esterhuizen, A.D., Lourens, J.G.H., Lindeque, H.W. et al. The effect of α-amylase on the acrosomal membrane of human sperm. J Assist Reprod Genet 12, 283–287 (1995). https://doi.org/10.1007/BF02212933

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Key words

  • α-amylase
  • human sperm
  • hemizona assay
  • acrosome reaction