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In vitro maturation and fertilization of oocytes isolated from aged mice: A strategy to rescue valuable genetic resources

Abstract

Purpose

This project was to determine whether oocytes isolated from virgin aged mice, up to 18 months old, are competent to undergo cytoplasmic maturation in vitro and undergo fertilization and embryonic development. If so, oocyte maturation in vitro could be used as a strategy to rescue valuable genetic resources.

Results

Although the number of oocytes recovered from mice was greatly reduced with increasing age, the percentage of oocytes that underwent fertilization, cleavage, and development to the blastocyst stage was essentially unchanged up to 18 months of age. The success of cleavage to the two-cell stage was greater after maturation in vitro (81%) than gonadotropin-induced maturation in vivo (55%). About 20% (20/106) of the embryos derived from oocytes isolated from 18-month-old mice developed to term after embryo transfer.

Conclusion

Oocytes from virgin aged mice undergo normal cytoplasmic maturation in vitro. Higher percentages of oocytes from aged mice cleave to the two-cell stage after spontaneous maturation in vitro than after gonadotropin-induced maturation in vivo. Therefore, in vitro maturation and fertilization of oocytes could be used to rescue valuable genetic resources that might otherwise be lost because of age-related infertility.

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References

  1. 1.

    Gordon JW, Talansky BE: Assisted fertilization by zona drilling: A mouse model for correction of oligospermia. J Exp Zool 1986;239:347–354

  2. 2.

    Mann JR: Full term development of mouse eggs fertilized by a spermatozoon microinjected under the zona pellucida. Biol Reprod 1988;38:1077–1083

  3. 3.

    Foote RH: The gametogenic function of the aging ovary in the mammal.In Aging Gametes, RJ Blandau (ed). Seattle, S. Karger AG, Basel, 1975, pp 179–200

  4. 4.

    Peluso JJ, England-Charlesworth C, Hutz R: Effect of age and of follicular aging on the preovulatory oocyte. Biol Reprod 1980;22:999–1005

  5. 5.

    Eppig JJ, Schultz RM, O'Brien M, Chesnel F: Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994;164:1–9

  6. 6.

    Chandley AC: Maternal aging as the important etiological factor in human aneuploidy.In Aneuploidy—Etiology and Mechanisms, VL Dellarco, PE Voytek, A Hollaender (eds). New York, Plenum Press, 1985, pp 409–416

  7. 7.

    Hoffmann GR: Etiology and mechanisms of aneuploidy: A synopsis.In Aneuploidy—Etiology and Mechanisms, VL Dellarco, PE Voytek, A Hollaender (eds). New York, Plenum Press, 1985, pp 539–548

  8. 8.

    Eichenlaub-Ritter U, Boll I: Nocodazole sensitivity, age-related aneuploidy, and alterations in the cell cycle during maturation of mouse oocytes. Cytogenet Cell Genet 1989;52:170–176

  9. 9.

    Gosden RG: Chromosomal anomalies of preimplantation mouse embryos in relation to maternal age. J Reprod Fert 1973;35:351–354

  10. 10.

    Pease S, Schroeder AC, Schmidt GH: Production of transgenic mice: Acupuncture needle-facilitated embryo transfer to oviduct ampulla. Trends Genet 1989;5:293.

  11. 11.

    Eppig JJ, Schroeder AC, O'Brien MJ: Developmental capacity of mouse oocytes matured in vitro: Effects of gonadotropic stimulation, follicular origin, and oocyte size. J Reprod Fert 1992;95:119–127

  12. 12.

    Eppig JJ, Wigglesworth K: Atypical maturation of oocytes of strain I/LnJ mice. Hum Reprod 1994;9:1136–1142

  13. 13.

    Ho Y, Wigglesworth K, Eppig JJ, Schultz RM: Preimplantation development of mouse embryos in KSOM: Augmentation by amino acids and analysis of gene expression. Mol Reprod Dev 1995 (in press)

  14. 14.

    Eppig JJ, Wigglesworth K, O'Brien MJ: Comparison of embryonic developmental competence of mouse oocytes grown with and without serum. Mol Reprod Dev 1992;32:33–40

  15. 15.

    Schroeder AC, Eppig JJ: The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev Biol 1984;102:493–497

  16. 16.

    Parkening TA, Collins TJ, Smith ER: Plasma and pituitary concentrations of LH, FSH and prolactin in aged female C57BL/6 mice. J Reprod Fert 1980;58:377–386

  17. 17.

    Gee DM, Flurkey K, Finch CE: Aging and the regulation of luteinizing hormone in C57BL/6J mice: Impaired elevations after ovariectomy and spontaneous elevations at advanced ages. Biol Reprod 1983;28:598–607

  18. 18.

    Lerner SP, Thayne WV, Baker RD, Henschen T, Meredith S, Inskeep EK, Dailey RA, Lewis PE, Butcher RL: Age, dose of FSH and other factors affecting superovulation in Holstein cows. J Anim Sci 1986;63:176–183

  19. 19.

    Garcia-Winder M, Lewis PE, Bryner RW, Baker RD, Inskeep EK, Butcher RL: Effect of age and norgestomet on endocrine parameters and production of embryos in superovulated beef cows. J Anim Sci 1988;66:1974–1981

  20. 20.

    Gosden RG: Survival of transferred C57B1 mouse embryos: Effects of age of donor and recipient. Fertil Steril 1974;25:348–351

  21. 21.

    Biggers JD, Finn CA, McLaren A: Long-term reproductive performance of female mice. II. Variation of litter size with parity. J Reprod Fertil 1962;3:313–330

  22. 22.

    Tease C, Fisher G: Oocytes from young and old female mice respond different to colchicine. Mutat Res 1986;173:31–34

  23. 23.

    Eichenlaub-Ritter U, Boll I: Age-related non-disjunction, spindle formation and progression through maturation of mammalian oocytes.In Mechanisms of Chromosome Distribution and Aneuploidy. New York, Alan R. Liss, 1989, pp 259–269

  24. 24.

    Schroeder AC, Johnston D, Eppig JJ: Reversal of postmortem degeneration of mouse oocytes during meiotic maturation in vitro. J Exp Zool 1991;258:240–245

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Correspondence to John J. Eppig.

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Eppig, J.J., O'brien, M. In vitro maturation and fertilization of oocytes isolated from aged mice: A strategy to rescue valuable genetic resources. J Assist Reprod Genet 12, 269–273 (1995). https://doi.org/10.1007/BF02212930

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Key words

  • genetic rescue
  • oocyte maturation
  • maturation in vitro
  • aging
  • mouse