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Morphology in intracytoplasmic sperm injection: Preliminary results

Abstract

Purpose

Direct intracytoplasmic sperm injection (ICSI) is a successful treatment of severe male subfertility. Conventional in vitrofertilization shows poor pregnancy rates especially in cases of severe teratozoospermia. The objective was to determine if severe morphological defects of spermatozoa in oligoasthenoteratozoospermia (OAT) have any impact on the fertilization process in ICSI and if there are any specific morphological characteristics in nonfertilized oocytes after the ICSI procedure.

Methods

Ejaculates and nonfertilized oocytes were investigated by electron microscopy.

Conclusions

The lack of intracytoplasmic sperm-oocyte interaction, not severe sperm defects, displays the most critical role in the fertilization process. Clinical data with fertilization rates of 66% and pregnancy rates of 23.3% confirm the fertilization capacity of severely defective spermatozoa in ICSI.

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References

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Author information

Correspondence to W. Küpker.

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Küpker, W., Al-Hasani, S., Schulze, W. et al. Morphology in intracytoplasmic sperm injection: Preliminary results. J Assist Reprod Genet 12, 620–626 (1995). https://doi.org/10.1007/BF02212586

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Key words

  • ICSI
  • OAT syndrome
  • ultrastructure of spermatozoa and oocytes