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Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation

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To study the early pathogenesis of acute edematous pancreatitis in dogs, we examined the relationship of pancreatic hyperstimulation with cholecystokinin-8 (10 μg/kg/hr intravenously for 6 hr) to alterations in circulating pancreatic enzymes and pancreatic morphology with special reference to trypsinogen activation. Cholecystokinin-8 infusion was associated with increases in plasma amylase, lipase, trypsin-like immunoreactivity, and plasma and urine trypsinogen activation peptide. Pancreatic parenchymal swelling and interlobular and subcapsular fluid accumulations were detected ultrasonographically within 2 hr of cholecystokinin-8. Circulating trypsin-like immunoreactivity and trypsinogen activation peptide in urine reached a peak at 2 and 4 hr, respectively, then declined despite progressive increases in circulating amylase and lipase and intrapancreatic fluid. No significant changes were observed in dogs receiving a saline infusion. This study illustrates that cholecystokinin-8 induces edematous pancreatitis in dogs that is associated with a short-lived burst of trypsinogen activation.

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Correspondence to Dr. K. W. Simpson BVMTS, PhD.

Additional information

Financial support for this study was provided in part by the Wellcome Trust.

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Simpson, K.W., Beechey-Newman, N., Lamb, C.R. et al. Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation. Digest Dis Sci 40, 2152–2161 (1995). https://doi.org/10.1007/BF02208999

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Key Words

  • acute pancreatitis
  • trypsinogen activation
  • trypsinogen activation peptide
  • pancreatic enzymes
  • dogs