Digestive Diseases and Sciences

, Volume 40, Issue 10, pp 2152–2161

Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation

  • K. W. Simpson
  • N. Beechey-Newman
  • C. R. Lamb
  • J. B. A. Smyth
  • G. Hughes
  • K. Coombe
  • N. Sumar
  • J. Hermon-Taylor
Pancreatic and Biliary Disorders

Abstract

To study the early pathogenesis of acute edematous pancreatitis in dogs, we examined the relationship of pancreatic hyperstimulation with cholecystokinin-8 (10 μg/kg/hr intravenously for 6 hr) to alterations in circulating pancreatic enzymes and pancreatic morphology with special reference to trypsinogen activation. Cholecystokinin-8 infusion was associated with increases in plasma amylase, lipase, trypsin-like immunoreactivity, and plasma and urine trypsinogen activation peptide. Pancreatic parenchymal swelling and interlobular and subcapsular fluid accumulations were detected ultrasonographically within 2 hr of cholecystokinin-8. Circulating trypsin-like immunoreactivity and trypsinogen activation peptide in urine reached a peak at 2 and 4 hr, respectively, then declined despite progressive increases in circulating amylase and lipase and intrapancreatic fluid. No significant changes were observed in dogs receiving a saline infusion. This study illustrates that cholecystokinin-8 induces edematous pancreatitis in dogs that is associated with a short-lived burst of trypsinogen activation.

Key Words

acute pancreatitis trypsinogen activation trypsinogen activation peptide pancreatic enzymes dogs 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • K. W. Simpson
    • 1
    • 2
  • N. Beechey-Newman
    • 1
    • 2
  • C. R. Lamb
    • 1
    • 2
  • J. B. A. Smyth
    • 1
    • 2
  • G. Hughes
    • 1
    • 2
  • K. Coombe
    • 1
    • 2
  • N. Sumar
    • 1
    • 2
  • J. Hermon-Taylor
    • 1
    • 2
  1. 1.Departments of Small Animal Medicine and Surgery, and PathologyRoyal Veterinary CollegeU.K.U.K.
  2. 2.Departments of SurgeryGuy's and St. George's Hospital Medical Schools, University of LondonU.K.
  3. 3.Department of Clinical Sciences, College of Veterinary MedicineCornell UniversityIthaca

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