Current Genetics

, Volume 29, Issue 4, pp 395–403

The identification and characterization of four laccases from the plant pathogenic fungusRhizoctonia solani

  • Jill A. Wahleithner
  • Feng Xu
  • Kim M. Brown
  • Stephen H. Brown
  • Elizabeth J. Golightly
  • Torben Halkier
  • Sakari Kauppinen
  • Anders Pederson
  • Palle Schneider
Original Paper

DOI: 10.1007/BF02208621

Cite this article as:
Wahleithner, J.A., Xu, F., Brown, K.M. et al. Curr Genet (1996) 29: 395. doi:10.1007/BF02208621

Abstract

Four distinct laccase genes,lcc1, lcc2, lcc3 andlcc4, have been identified in the fungusRhizoctonia solani. Both cDNA and genomic copies of these genes were isolated and characterized. Hybridization analyses indicate that each of the four laccase genes is present in a single copy in the genome. TheR. solani laccases can be divided into two groups based on their protein size, intron/exon organization, and transcriptional regulation. Three of these enzymes have been expressed in the fungusAspergillus oryzae. Two of the recombinant laccases, r-lccl and r-lcc4, as well as the native lcc4 enzyme were purified and characterized. The purified proteins are homodimeric, comprised of two subunits of approximately 66 kDa for lcc4 and 50–100 kDa for the recombinant lccl protein. These laccases have spectral properties that are consistent with other blue copper proteins. With syringaldazine as a substrate, lcc4 has optimal activity at pH 7, whereas lcc1 has optimal activity at pH 6.

Key words

Laccas Phenol-oxidas Rhizoctonia solani Fungi 

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Jill A. Wahleithner
    • 1
  • Feng Xu
    • 1
  • Kim M. Brown
    • 1
  • Stephen H. Brown
    • 1
  • Elizabeth J. Golightly
    • 1
  • Torben Halkier
    • 2
  • Sakari Kauppinen
    • 2
  • Anders Pederson
    • 2
  • Palle Schneider
    • 2
  1. 1.Novo Nordisk BiotechDavisUSA
  2. 2.Novo Nordisk A/SBagsævrdDenmark

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