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Current Genetics

, Volume 29, Issue 4, pp 327–334 | Cite as

The response regulator-like protein Pos9/Skn7 ofSaccharomyces cerevisiae is involved in oxidative stress resistance

  • Bernhard Krems
  • Christina Charizanis
  • Karl-Dieter Entian
Original Paper

Abstract

We have isolated mutants ofSaccharomyces cerevisiae with an increased sensitivity to oxidative stress. Allpos9 mutants (pos for peroxide sensitivity) were hypersensitive to methylviologene, hyperbaric oxygen or hydrogen peroxide, but grew similarly to the wild-type under all other conditions tested. Isolation and sequencing of the respectivePOS9 gene revealed that it was identical toSKN7. The predicted Skn7/Pos9 protein possesses a domain with high homology to prokaryotic response regulators. These regulatory proteins are part of a simple signalling cascade termed a “two-component system”, where a phosphorylation signal of a histidine kinase is transferred to a conserved aspartate residue of the response regulator. To test the functional role of the respective aspartate residue of Skn7/Pos9 protein in oxidative stress, we mutagenized this residue in vitro to alanine, arginine and glutamate. Only the glutamate allele (D427 to E) was able to rescue the hydrogen peroxide-sensitivity ofpos9 mutants. By fusion experiments with the Gal4 DNA-binding domain we identified the isolated response regulator-like domain as a novel eukaryotic domain sufficient for gene activation. Whereas this hybrid protein activated transcription of alacZ reporter gene under aerobic conditions, no activation was observed under anaerobic conditions, indicating that the response regulator domain is involved in a signalling reaction. Two-hybrid investigations also suggest an oligomerization of the Pos9 protein. Our results indicate that a two-component system is involved in the oxidative-stress response of yeast.

Key words

Response regulator Transcriptional activation Oxidative stress 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Bernhard Krems
    • 1
  • Christina Charizanis
    • 1
  • Karl-Dieter Entian
    • 1
  1. 1.Institute for MicrobiologyJohann Wolfgang Goethe-UniversityFrankfurtGermany

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