Clinical Rheumatology

, Volume 14, Issue 6, pp 673–677 | Cite as

Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase

  • M. Miyata
  • A. Kogure
  • H. Sato
  • E. Kodama
  • H. Watanabe
  • H. Ohira
  • M. Kuroda
  • T. Takagi
  • Y. Sato
  • R. Kasukawa
Originals

Summary

The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of mycobacterial HSP65.

Key words

Autoimmune Hepatitis Mycobacterial 65 KD Heat Shock Protein Superoxide Dismutase Human 60 KD Heat Shock Protein Molecular Mimicry 

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Copyright information

© Acta Medica Belgica 1995

Authors and Affiliations

  • M. Miyata
    • 1
  • A. Kogure
    • 1
  • H. Sato
    • 1
  • E. Kodama
    • 1
  • H. Watanabe
    • 1
  • H. Ohira
    • 1
  • M. Kuroda
    • 1
  • T. Takagi
    • 1
  • Y. Sato
    • 1
  • R. Kasukawa
    • 1
  1. 1.Department of Internal Medicine IIFukushima Medical CollegeFukushimaJAPAN

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