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Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells

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The effects of extracellular adenosine 5′-triphosphate (ATP) on voltage-dependent Ca2+ currents were examined using the whole-cell voltage-clamp technique in guinea-pig isolated adrenal chromaffin cells. ATP (500 μM) reversibly suppressed Ca2+ currents in the presence of 5 mM Ca2+ in the extracellular solution. The inhibitory effect of ATP on Ca2+ currents tended to increase with increases in the peak amplitude of ATP-evoked current when the intracellular solution contained 0.1 or 1 mM ethylenebis(oxonitrilo)tetraacetate(EGTA). Using the intracellular solution containing 10 mM EGTA, on the other hand, the inhibitory efftect did not change regardless of the amplitude of current responses to ATP In the presence of 10 mM Ba2+, ATP (100 μmol/l). reduced Ba2+ currents in a manner similar to Ca2+ currents. This reduction was decreased by dialysis of cells with the internal solution containing guanosine 5′-O-(2-thiodiphosphate) (GDP [β-S]; 1 mM) or guanosine 5′-O-(3-thiotriphos-phate) (GTP [γ-S]; 100 μmol/l). A depolarizing prepulse channels. In addition, ATP seems to modulate Ca2+ channels via the pathway related to G-protein. Adenine nucleotides and adenosine may play a role in controlling secretory activity in guinea-pig adrenal chromaffin cells.

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Correspondence to Yoshikazu Nakazato.

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Otstuguro, K., Ohta, T., Ito, S. et al. Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells. Pflugers Arch. 431, 402–407 (1996).

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Key words

  • Adenine nucleotide
  • Adenosine Chromaffin cells
  • Calcium channels
  • G-prooein
  • Whole-cell voltage-clamp