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Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells

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Abstract

The effects of extracellular adenosine 5′-triphosphate (ATP) on voltage-dependent Ca2+ currents were examined using the whole-cell voltage-clamp technique in guinea-pig isolated adrenal chromaffin cells. ATP (500 μM) reversibly suppressed Ca2+ currents in the presence of 5 mM Ca2+ in the extracellular solution. The inhibitory effect of ATP on Ca2+ currents tended to increase with increases in the peak amplitude of ATP-evoked current when the intracellular solution contained 0.1 or 1 mM ethylenebis(oxonitrilo)tetraacetate(EGTA). Using the intracellular solution containing 10 mM EGTA, on the other hand, the inhibitory efftect did not change regardless of the amplitude of current responses to ATP In the presence of 10 mM Ba2+, ATP (100 μmol/l). reduced Ba2+ currents in a manner similar to Ca2+ currents. This reduction was decreased by dialysis of cells with the internal solution containing guanosine 5′-O-(2-thiodiphosphate) (GDP [β-S]; 1 mM) or guanosine 5′-O-(3-thiotriphos-phate) (GTP [γ-S]; 100 μmol/l). A depolarizing prepulse channels. In addition, ATP seems to modulate Ca2+ channels via the pathway related to G-protein. Adenine nucleotides and adenosine may play a role in controlling secretory activity in guinea-pig adrenal chromaffin cells.

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References

  1. 1.

    Aicardi G, Pollo A, Sher E, Carbone E (1991) Noradrenergic inhibition and voltage-dependent facilitation ofω-conotoxin-sensitive Ca channels in insulin- secreting RIFm5F cells. FEBS Lett 281:201–204

  2. 2.

    Albillos A, Artalejo AR, López MG, Gandía L, García AG, Carbone E (1994) Calcium channel subtypes in cat chromaffin cells. J Physiol (Lond) 477:197–213

  3. 3.

    Artalejo CR, Dahmer MK, Perlman RL, Fox AP (1991) Two types of Ca2+ currents are found in bovine chromaffin cells: facilitation is due to the recruitment of one type. J Physiol (Lond) 432:681–707

  4. 4.

    Asano T, Otsuguro K, Ohta T, Sugawara T, Nakazato Y (1995) Characterization of ATP-induced catecholamine secretion from adrenal chromaffin cells of the guniea-pig. Comp Biochem Physiol (in press)

  5. 5.

    Bean BP (1992) Whole-cell recording of calcium channel currents. In: Rudy B, Iverson LE (ed) Methods in enzymology. Academic, New York, pp 181–193

  6. 6.

    Chern YJ, Herrera M, Kao LS, Westhead EW (1987) Inhibition of catecholamine secretion from bovine chromaffin cells by adenine nucleotides and adenosine. J Neurochem 48:1573–1576

  7. 7.

    Chern YJ, Bott M, Chu PJ, Lin YJ, Kao LS, Westhead EW (1992) The adenosine analogueN 6-l-phenylisopropyladeno-sine inhibits catecholamine secretion from bovine adrenal medulla cells by inhibiting calcium influx. J Neurochem 59: 1399–1404

  8. 8.

    Dalziel HH, Westfall DP (1994) Receptors for adenine nucleotides and nucleosides: subclassification, distribution, and molecular characterization. Pharmacol Rev 46:449–466

  9. 9.

    Diverse-Pierluissi M, Dunlap K, Westhead EW (1991) Multiple actions of extracellular ATP on calcium currents in cultured bovine chromaffin cells. Proc Natl Acad Sci USA 88:1261–1265

  10. 10.

    Douglas WW, Poisner AM, Rubin RP (1965) Efflux of adenine nucleotides from perfused adrenal glands exposed to nicotine and other chromaffin cell stimulants. J Physiol (Lond) 179: 130–137

  11. 11.

    Doupnik CA, Pun RYK (1994) G-protein activation mediates prepulse facilitation of Ca2+ channel currents in bovine chromaffin cells. J Membr Biol 140:47–56

  12. 12.

    Eckert R, Chad JE (1984) Inactivation of Ca channels. Prog Biophys Mol Biol 44:215–267

  13. 13.

    Gandia L, Garcia AG, Morad M (1993) ATP modulation of calcium channels in chromaffin cells. J Physiol (Lond) 470: 55–72

  14. 14.

    Grassi F, Lux HD (1989) Voltage-dependent GABA-induced modulation of calcium currents in chick sensory neurons. Neurosci Lett 105:113–119

  15. 15.

    Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85–100

  16. 16.

    Hochman J, Perlman RL (1976) Catecholamine secretion by isolated adrenal cells. Biochim Biophys Acta 421:168–175

  17. 17.

    Ito S (1983) Time course of release of catecholamine and other granular contents from perifused adrenal chromaffin cells of guinea-pig. J Physiol (Lond) 341:153–167

  18. 18.

    Kasai H, Aosaki T (1989) Modulation of Ca-channel current by an adenosine analog mediated by a GTP-binding protein in chick sensory neurons. Pflügers Arch 414:145–149

  19. 19.

    Kim KT, Westhead EW (1989) Cellular responses to Ca2+ from extracellular and intracellular sources are different as shown by simultaneous measurements of cytosolic Ca2+ and secretion from bovine chromaffin cells. Proc Natl Acad Sci USA 86:9881–9885

  20. 20.

    Mateo J, Castro E, Zwiller J, Aunis D, Miras-Portugal MT (1995) 5′-(N-ethylcarboxamido) adenosine inhibits Ca2+ influx and activates a protein phosphatase in bovine adrenal chromaffin cells. J Neurochem 64:77–84

  21. 21.

    Nakazawa K, Fujimori K, Takanaka A, Inoue K (1990) An ATP-activated conductance in pheochromocytoma cells and its suppression by extracellular calcium. J Physiol (Lond) 428: 257–272

  22. 22.

    Ohta T, Asano T, Ito S, Noto T, Tachibana R, Nakazato Y, Ohga A (1992) The inhibitory action of cyclic AMP on responses to carbachol dependent on calcium stores in rat gastric smooth muscle. J Physiol (Lond) 453:367–384

  23. 23.

    Otsuguro K, Asano T, Ohta T, Ito S, Nakazato Y (1995) ATP-evoked membrane current in guinea pig adrenal chromaffin cells. Neurosci Lett 187:145–148

  24. 24.

    Salzman SK, Sellers MS (1982) Determination of norepinephrine in brain perfusates using high-performance liquid chromatography with electrochemical detection. J Chromatogr 232:29–37

  25. 25.

    Schneider P, Hopp HH, Isenberg G (1991) Ca2+ influx through ATP-gated channels increments [Ca+]i and inactivatesI ca in myocytes from guinea-pig urinary bladder. J Physiol (Lond) 440:479–496

  26. 26.

    Swandulla D, Carbone E, Lux HD (1991) Do calcium channel classifications account for neuronal calcium channel diversity? Trends Neurosci 14:46–51

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Correspondence to Yoshikazu Nakazato.

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Otstuguro, K., Ohta, T., Ito, S. et al. Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells. Pflugers Arch. 431, 402–407 (1996). https://doi.org/10.1007/BF02207278

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Key words

  • Adenine nucleotide
  • Adenosine Chromaffin cells
  • Calcium channels
  • G-prooein
  • Whole-cell voltage-clamp