Pflügers Archiv

, Volume 431, Issue 3, pp 395–401 | Cite as

Dose-dependent alteration of rat cardiac sodium current by isoproterenol: Results from direct measurements on multicellular preparations

  • Michael Kirstein
  • Kurt Kochsiek
  • Heiner Langenfeld
  • Roland Eickhorn
Original Article

Abstract

Conflicting results have been reported in literature about the influence ofβ-adrenergic stimulation on the fast cardiac sodium current (INa+). To elucidate these mechanisms in multicellular preparations we used the loose-patch-clamp technique to evaluate the effect of theβ-adrenergic agonist isoproterenol 1–1000 nmol/l. Isoproterenol enhancedINa+ at all membrane potentials by elevation of the maximal availableINa+. Only at the high concentration of 1 μmol/l wasINa+ slightly depressed after depolarizing conditioning clamps. The most marked increase of the maximal availableINa+ was 30 ± 9 % after application of 100 nmol/l isoproterenol. To learn about the mechanisms in view of sodium channel modulation we combined isoproterenol with the sodium channel blocker lidocaine (47 μmol/l). Under these circumstances the effects of both drugs were completely independent. This investigation shows clearly that low concentrations of isoproterenol increaseINa+ in multicellular preparations by a gating-independent mechanism.

Key words

Sodium current Adrenergic regulation Loose-patch-clamp Rat papillary muscle 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Michael Kirstein
    • 1
  • Kurt Kochsiek
    • 1
  • Heiner Langenfeld
    • 1
  • Roland Eickhorn
    • 2
  1. 1.Medical University ClinicWürzburgGermany
  2. 2.Department of PhysiologyUniversity of FreiburgFreiburgGermany

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