High-frequency transformation ofStaphylococcus aureus by electroporation
Plasmids carrying resistances for a variety of antibiotics were successfully transformed intoStaphylococcus aureus by electroporation. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 × 105 transformants/µg of DNA were obtained with DNA isolated from both large-scale plasmid preparations and from minilysate preparations. A procedure was also developed for preparation and freezing (−70°C) of electrocompetent cells. These preparations have remained electrotransformable for 2 months without loss of activity.
KeywordsCell Preparation Freeze Cell Plasmid Preparation Electrocompetent Cell ofStaphylococcus Aureus
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