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Current Microbiology

, Volume 21, Issue 6, pp 373–376 | Cite as

High-frequency transformation ofStaphylococcus aureus by electroporation

  • Ginger Rhoads Kraemer
  • John J. Iandolo
Article

Abstract

Plasmids carrying resistances for a variety of antibiotics were successfully transformed intoStaphylococcus aureus by electroporation. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 × 105 transformants/µg of DNA were obtained with DNA isolated from both large-scale plasmid preparations and from minilysate preparations. A procedure was also developed for preparation and freezing (−70°C) of electrocompetent cells. These preparations have remained electrotransformable for 2 months without loss of activity.

Keywords

Cell Preparation Freeze Cell Plasmid Preparation Electrocompetent Cell ofStaphylococcus Aureus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1990

Authors and Affiliations

  • Ginger Rhoads Kraemer
    • 1
  • John J. Iandolo
    • 1
  1. 1.Department of PathologyKansas State UniversityManhattanUSA

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