Plant and Soil

, Volume 68, Issue 3, pp 391–394

A method for determining concentration profiles at the soil-root interface by thin slicing rhizospheric soil

  • R. Kuchenbuch
  • A. Jungk
Short Communications

Summary

A method is described for the determination of concentration gradients in the vicinity of plant roots. Plants are grown in small containers in which the roots are separated from the soil by a screen of nylon cloth. Root hairs but not roots penetrate the screen into the soil. In order to investigate the rhizospheric soil, the soil is frozen by liquid nitrogen and sliced into layers about 0.06 mm thick by means of a refrigerated microtome.

Key words

Concentration gradients Rhizosphere Soil-root interface Soil slicing method 

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References

  1. 1.
    Bhat K K S and Nye P H 1973 Plant and Soil 38, 161–175.Google Scholar
  2. 2.
    Brown D A et al. 1964 Soil Sci. Soc. Am. Proc. 28, 628–632.Google Scholar
  3. 3.
    Claassen N et al. 1981 Z. Pflanzenernaehr. Bodenkd. 144, 306–316.Google Scholar
  4. 4.
    Claassen N. et al. 1981 Z. Pflanzenernaehr. Bodenkd. 144, 533–545.Google Scholar
  5. 5.
    Farr E et al. 1969 Soil Sci. 107, 385–391.Google Scholar
  6. 6.
    Hartge K H 1971 Die physikalische Untersuchung von Böden. Verlag F. Enke, Stuttgart.Google Scholar
  7. 7.
    Helal H M und Sauerbeck D 1981 Z. Pflanzenernaehr. Bodenkd. 144, 524–527.Google Scholar
  8. 8.
    Hendriks L et al. 1981 Z. Pflanzenernaehr. Bodenkd. 144, 486–499.Google Scholar
  9. 9.
    Hsieh J J C et al. 1972 Soil Sci. Soc. Am. Proc. 36, 418–421.Google Scholar
  10. 10.
    Jost W 1952 Diffusion in soils, liquids, gases. Academic Press Inc., New York.Google Scholar
  11. 11.
    Vaidyanathan L V and Nye P H 1966 J. Soil Sci. 17, 175–183.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1982

Authors and Affiliations

  • R. Kuchenbuch
    • 1
  • A. Jungk
    • 1
  1. 1.Institute for Plant NutritionUniversity of HannoverHannover 21

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