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Pharmaceutisch weekblad

, Volume 3, Issue 1, pp 1341–1348 | Cite as

Immunofluorescentiebepalingen, een overzicht

  • P. S. C. Van Der Plas
  • F. A. Huf
  • H. J. De Jong
Overzichtsartikelen
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Abstract

Immunoassays enables to perform highly specific and sensitive analyses in complex matrices. In this review the role of fluorescence immunoassay in relation to all other immunoassays is described. A comprehensive study of the principles of the fluorescence immunoassays is presented here. Reported applications are mentioned and the detection limits are indicated. Finally attention is paid to the requirements for fluorescent labels. Some of the most widely used labels are being discussed here.

Samenvatting

Immunologische bepalingen lenen zich bij uitstek voor het met grote specificiteit en gevoeligheid verrichten van analyses in complexe matrices. Dit overzicht beschrijft de rol van de immunofluorescentiebepalingen ten opzichte van de andere immunologische analysemethoden. Een uitgebreide bespreking van de principes van de diverse immunofluorescentiebepalingen volgt; de in de literatuur vermelde toepassingen worden weergegeven en de onderste detectiegrens van de betreffende techniek wordt aangegeven. Ten slotte wordt ingegaan op de eisen die aan fluorescerende labels moeten worden gesteld, en worden de meest gebruikte labels besproken.

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Copyright information

© Bohn, Scheltema & Holkema 1981

Authors and Affiliations

  • P. S. C. Van Der Plas
    • 1
  • F. A. Huf
    • 1
  • H. J. De Jong
    • 1
  1. 1.Vakgroep Farmaceutische Analyse en Analytische ChemieRijksuniversiteit LeidenRA Leiden

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