Pharmaceutisch weekblad

, Volume 3, Issue 1, pp 1309–1315 | Cite as

An HPLC method for the determination of salicylic acid, phenacetin and paracetamol in serum, with indications; two case-reports of intoxication

  • D. R. A. Uges
  • H. Bloemhof
  • E. K. Juul Christensen


Acetylsalicylic acid, paracetamol and phenacetin are the most frequently used non-narcotic analgesics in The Netherlands. Two new case reports, an intoxication by salicylate ointment and a paracetamol overdose, are described.

Indications and methods for the determination of these compounds are discussed. We describe a newHplc method for the simultaneous determination of salicylic acid, paracetamol and phenacetin in serum. Some other analgesics, like aminophenazone, phenazone and dipyrone can be determined by this method at the same time, 100Μl of serum is mixed with 600Μl methanol, centrifuged and an aliquot is injected into theRP-5C18Hplc column. A methanol—buffer pH=3.0 (40 + 50) mixture is used as mobile phase. Salicylamide can be used as internal standard. Coefficients of variation of 3.4% for salicylic acid, 1.9% for paracetamol and 3.4% for phenacetin are found and recoveries from calf's serum are 98–101% (n=10 per drug at 50 mg/l serum).

In neonatology the sample volume can be decreased from 100Μl to 10Μl. This method has proved to be reliable and suitable in clinical pharmacy and toxicology.


Salicylic Acid Paracetamol Salicylate Acetylsalicylic Acid Phenacetin 
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  1. Blair, D., andB.H. Rumack (1977)Clin. Chem. 23, 743.PubMedGoogle Scholar
  2. Blair, D., B.H. Rumack andR.G. Peterson (1978)Clin. Chem. 24, 1543.PubMedGoogle Scholar
  3. Bouma, P., andD.R.A. Uges (1980) In:The Serum Concentration of Drugs (Merkus, F.W.H.M., Ed.). Excerpta Medica, Amsterdam, 278.Google Scholar
  4. Brouwers, J.R.B.J. (1978)Pharm. Weekblad 113, 129.Google Scholar
  5. Clarke, E.G.C. (1969)Isolation and Identification of Drugs. The Pharmaceutical Press, London, 131.Google Scholar
  6. Cumming, A.J., andB.K. Martin (1968)J. Pharm. Sci. 57, 891.CrossRefGoogle Scholar
  7. Dijkhuis, I.C. (1979) Ph.D. thesis, Leiden.Google Scholar
  8. Dijkhuis, I.C., E.W. De Flines andJ.G. Rengerink (1979)Pharm. Weekblad Sci. Ed. 1, 185.Google Scholar
  9. Evans, M.A., andR.D. Harbinson (1977)J. Pharm. Sci. 66, 1628.CrossRefPubMedGoogle Scholar
  10. Glynn, J.P., andS.E. Kendall (1975)Lancet I, 1147.CrossRefGoogle Scholar
  11. Harders, C.L., J. Glerum andJ.C. Kutsch Lojenga (1972)Mededelingen NVZA 28, 24.Google Scholar
  12. Heyst, A.N.P. van (1981) Personal communication.Google Scholar
  13. Heyst, A.N.P. Van, andS.A. Pikaar (1980)Vademecum Vergiftigingen, 2nd edition. Elsevier Argus, Amsterdam.Google Scholar
  14. Howie, D., P.I. Adriaenssens andL.F. Prescott (1977)J. Pharm. Pharmacol. 29, 235.CrossRefPubMedGoogle Scholar
  15. Levy, G., T. Lampman;B.L. Kamath andL.K. Garrettson (1975)New. Engl. J. Med. 293, 323.CrossRefPubMedGoogle Scholar
  16. Levy, G., andK.M. Giacomini (1978)Clin. Pharmacol. Therap. 23, 247.CrossRefGoogle Scholar
  17. Levy, G., andT. Tsuchiya (1972)New Engl. J. Med. 287, 430.CrossRefPubMedGoogle Scholar
  18. Liu, T.Z., andK.H. Oka (1980)Clin. Chem. 26, 69.PubMedGoogle Scholar
  19. Øie, S., andK. Frislid (1971)Pharm. Acta. Helv. 46, 632.PubMedGoogle Scholar
  20. Prescott, L.F. (1971)J. Pharm. Pharmacol. 23, 111CrossRefPubMedGoogle Scholar
  21. Pérez-Mateo, M., S. Erill andR. Cabezas (1977)Int. J. Clin. Pharmacol. Biopharm. 15, 113.PubMedGoogle Scholar
  22. Robinson, B.A., W.G. Sherwood, J. Tayler, J.W. Balfe andO.A. Mamer (1979)J. Pediatr. 95 (2), 228.CrossRefPubMedGoogle Scholar
  23. Roth, G.J., N. Stanford andP.W. Majerus (1975)Proc. Natl. Acad. Sci. U.S.A. 72, 3073.CrossRefPubMedPubMedCentralGoogle Scholar
  24. Stevenson, G.W. (1960)Analyt. Chem. 32, 1522.CrossRefGoogle Scholar
  25. Stewart, M.J., andJ.W. Barclay (1976)Lancet II, 362.CrossRefGoogle Scholar
  26. Sunshine, I. (1975)Methodology for Analytical Toxicology, ed. I.CRC Press Inc., Cleveland, Ohio, USA.Google Scholar
  27. Tischio, J.P. (1976)J. Pharm. Sci. 65, 1350.CrossRefGoogle Scholar
  28. Thomas, B.H., andB.B. Coldwell (1972)J. Pharm. Pharmacol. 24, 243.CrossRefPubMedGoogle Scholar
  29. Trinder, P. (1954)Biochem. J. 57, 301.CrossRefPubMedPubMedCentralGoogle Scholar
  30. Uges, D.R.A. (1981) Unpublished observations.Google Scholar
  31. Uges, D.R.A., andP. Bouma (1979)Pharm. Weekblad Sci. Ed. 1, 45.CrossRefGoogle Scholar
  32. Uges, D.R.A., A. Steenhoek, P.Th.M. Ten Doeschot andE.K. Juul Christensen (1979)Medelingen NVZA 37, 383.Google Scholar
  33. Wallace, J.E., J.D. Biggs, H.E. Hamilton, L.L. Foster andK. Blum (1973)J. Pharm. Sci. 62, 599.CrossRefPubMedGoogle Scholar
  34. Wenger, T.L., andJ.H. Hull (1980)New Engl. J. Med. 303, 1121.PubMedGoogle Scholar
  35. Widdop, B., andR. Goulding (1976)Lancet II, 583.CrossRefGoogle Scholar
  36. Wong, L.T., G. Solomonraj andB.H. Thomas (1976)J. Pharm. Sci. 65, 1064.CrossRefPubMedGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1981

Authors and Affiliations

  • D. R. A. Uges
    • 1
  • H. Bloemhof
    • 1
  • E. K. Juul Christensen
    • 1
  1. 1.Laboratory for Toxicology and Clinical Pharmacy, Department of PharmacyUniversity HospitalRB GroningenThe Netherlands

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