Pharmaceutisch weekblad

, Volume 3, Issue 1, pp 582–586 | Cite as

HPLC-estimation of tobramycin in serum

  • P. G. L. C. Krugers Dagneaux
  • J. T. Klein Elhorst
Original Articles


This paper describes a method for estimating tobramycin in serum with reversed phase high-pressure liquid chromatography (HPLC) and fluorescence detection. Sisomicin as an internal standard, and perchloric acid are added to the serum. After centrifuging, the clear supernatant is directly injected onto a Lichrosorb RP-18 column. The elution is performed by means of a gradient of two solvents. Postcolumn the eluate is mixed continuously with ano-phthalaldehyde reagent and the fluorescence is measured. Good correlation is obtained with aRia-method and a classic microbiological estimation method. The method is suitable for clinical use in routine laboratories.


Quinidine Tobramycin Disopyramide Sisomicin Reaction Coil 
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Copyright information

© Bohn, Scheltema & Holkema 1981

Authors and Affiliations

  • P. G. L. C. Krugers Dagneaux
    • 1
  • J. T. Klein Elhorst
    • 1
  1. 1.Ziekenhuis De StadsmatenJX EnschedeThe Netherlands

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