HPLC-estimation of tobramycin in serum
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This paper describes a method for estimating tobramycin in serum with reversed phase high-pressure liquid chromatography (HPLC) and fluorescence detection. Sisomicin as an internal standard, and perchloric acid are added to the serum. After centrifuging, the clear supernatant is directly injected onto a Lichrosorb RP-18 column. The elution is performed by means of a gradient of two solvents. Postcolumn the eluate is mixed continuously with ano-phthalaldehyde reagent and the fluorescence is measured. Good correlation is obtained with aRia-method and a classic microbiological estimation method. The method is suitable for clinical use in routine laboratories.
KeywordsQuinidine Tobramycin Disopyramide Sisomicin Reaction Coil
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