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Journal of Biomolecular NMR

, Volume 1, Issue 4, pp 439–446 | Cite as

Measurement of15N-13C J couplings in staphylococcal nuclease

  • Frank Delaglio
  • Dennis A. Torchia
  • Ad Bax
Research Papers

Summary

15N-Cα and15N-C′ J couplings were measured for the backbone of staphylococcal nuclease, uniformly enriched with15N and13C. It is found that theIJC'N coupling is similar for β-sheet, J=14.8 ± 0.5 and for α-helix, J = 14.8 ± 0.4 but tends to be larger for the unstructured N- and C-terminal ends of the protein (J=15.6 ± 0.5). On average,1JNCα are smaller for α-helical residues (J=9.6 ± 0.3 Hz) compared to β-sheet (J=10.9 ± 0.8 Hz) and a substantial difference is observed for2JNCα in α-helices (J=6.4 ± 0.4 Hz) and β-sheets (J=8.3 ± 0.8 Hz).

Keywords

2D NMR J couplings Protein structure Isotopic labeling Triple resonance 

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Copyright information

© ESCOM Science Publishers B.V 1991

Authors and Affiliations

  • Frank Delaglio
    • 1
  • Dennis A. Torchia
    • 2
  • Ad Bax
    • 1
  1. 1.Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaUSA
  2. 2.Bone Research Branch, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

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