Molecular and General Genetics MGG

, Volume 248, Issue 6, pp 712–718

Dosage suppressors of the dominant G1 cyclin mutantCLN3-2: Identification of a yeast gene encoding a putative RNA/ssDNA binding protein

  • Katsunori Sugimoto
  • Kunihiro Matsumoto
  • Roger D. Kornberg
  • Steven I. Reed
  • Curt Wittenberg
Original Paper


Three G1 cyclins,CLN1,CLN2, andCLN3, have been identified in the budding yeastSaccharomyces cerevisiae. G1 cyclins are essential, albeit functionally redundant, rate-limiting activators of cell cycle initiation. We have isolated dosage-dependent suppressor genes (designatedHMD genes) of the mating defect caused byCLN3-2, a dominant mutation inCLN3,HMD2 andHMD3 are identical toSTE4 andSTE5, respectively,HMD1 is an essential gene that encodes a protein containing a putative RNA binding domain. Overproduction ofHMD1 results in a relatively specific reduction in the level of theCLN3 orCLN3-2 transcript. This reduction occurs subsequent to transcription initiation ofCLN3 since overexpression ofHMD1 did not affect expression of a heterologous transcript from theCLN3 promoter but did result in a reduction ofCLN3 transcript expressed from a heterologous promoter.HMD1 has at least one essential role independent of its effect onCLN3 sinceHMD1 remains essential for viability in the absence of a functionalCLN3 gene.

Key words

G1 cyclin RNA binding protein Saccharomyces cerevisiae 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Katsunori Sugimoto
    • 1
  • Kunihiro Matsumoto
    • 1
  • Roger D. Kornberg
    • 2
  • Steven I. Reed
    • 3
  • Curt Wittenberg
    • 3
  1. 1.Department of Molecular Biology, School of ScienceNagoya UniversityChikusa-ku, NagoyaJapan
  2. 2.Department of Cell BiologyStanford University School of MedicineStanfordUSA
  3. 3.Department of Molecular BiologyThe Scripps Research InstituteLa JollaUSA

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