Molecular and General Genetics MGG

, Volume 248, Issue 6, pp 644–648 | Cite as

A selection system for diploid and against haploid cells inSchizosaccharomyces pombe

  • Yoshie Tange
  • Osami Niwa
Original Paper

Abstract

We have isolated a mutant ofSchizosaccharomyces pombe whose growth is temperature sensitive when it is haploid but not when it is diploid. This mutant may provide a useful system for selecting nonconditional mutants which are defective in diploid formation upon conjugation.

Key words

Temperature-sensitive mutation Schizosaccharomyces pombe Haploid Diploid 

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References

  1. Alfa C, Fantes P, Hyams J, Mcleod M, Warbrick E (1993) Experiments with fission yeast: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  2. de Barros Lopes M, Ho J-Y, Reed SI (1990) Mutations in cell division cycle genesCDC36 andCDC39 activate the Saccharomyces cerevisiae mating pheromone response pathway. Mol Cell Biol 10:2966–2972Google Scholar
  3. Chikashige Y, Ding D-Q, Funabiki H, Haraguchi T, Mashiko S, Yanagida M, Hiraoka Y (1994) Telomere-led premeiotic chromosome movement in fission yeast. Science 264:270–273Google Scholar
  4. Costello G, Rodgers L, Beach D (1986) Fission yeast enters the stationary phase G0 from either mitotic G1 or G2. Curr Genet 11:119–125Google Scholar
  5. Dietzel C, Kurjan J (1987) The yeast SCG1 gene: a Gα-like protein implicated in thea- and α-factor response pathway. Cell 50:1001–1010Google Scholar
  6. Funabiki H, Hagan I, Uzawa S, Yanagida M (1993) Cell cyledependent positioning and clustering of centromeres and telomeres in fission yeast. J Cell Biol 121:961–976Google Scholar
  7. Goebl MG, Petes TD (1986) Most of the yeast genomic sequences are not essential for cell growth and division. Cell 46:983–992Google Scholar
  8. Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King (ed) Handbook of genetics. Plenum Press, New York, pp 395–446Google Scholar
  9. Kaback DB, Oeller PW, Steensma HY, Hirschman J, Ruezinsky D, Coleman KG, Pringle JR (1984) Temperature-sensitive lethal mutations in yeast chromosome 1 appear to define only a small number of genes. Genetics 108:67–90Google Scholar
  10. Miyajima I, Nakafuku M, Nakayama N, Brenner C, Miyajima A, Kaibuchi K, Arai K-I, Kaziro Y, Matsumoto K (1987) GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 50:1011–1019Google Scholar
  11. Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeastSchizosaccharomyces pombe. Methods Enzymol 194:795–823Google Scholar
  12. Niwa O, Yanagida M (1985) Triploid meiosis and aneuploidy inSchizosaccharomyces pombe: an unstable aneuploid disomic for chromosome III. Curr Genet 9:463–470Google Scholar
  13. Scherthan H, Bahler J, Kohli J (1994) Dynamics of chromosome organization and pairing during meiotic prophase in fission yeast. J Cell Biol 127:273–285Google Scholar
  14. Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  15. Uemura T, Yanagida M (1984) Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J 3:1737–1744Google Scholar
  16. Weiner BM, Kleckner N (1994) Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast. Cell 77:977–991Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Yoshie Tange
    • 1
  • Osami Niwa
    • 1
  1. 1.Kazusa DNA Research InstituteChibaJapan

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