Advertisement

Molecular and General Genetics MGG

, Volume 248, Issue 3, pp 278–286 | Cite as

QTL analysis of flowering time inArabidopsis thaliana

  • Jonathan H. Clarke
  • Richard Mithen
  • James K. M. Brown
  • Caroline Dean
Original Paper

Abstract

Quantitative trait loci (QTL) analyses based on restriction fragment length polymorphism maps have been used to resolve the genetic control of flowering time in a cross between twoArabidopsis thaliana ecotypes H51 and Landsbergerecta, differing widely in flowering time. Five quantitative trait loci affecting flowering time were identified in this cross (RLN1-5), four of which are located in regions containing mutations or loci previously identified as conferring a late-flowering phenotype. One of these loci is coincident with theFRI locus identified as the major determinant for late flowering and vernalization responsiveness in theArabidopsis ecotype Stockholm.RLN5, which maps to the lower half of chromosome five (between markers mi69 and m233), only affected flowering time significantly under short day conditions following a vernalization period. The late-flowering phenotype of H51 compared to Landsbergerecta was due to alleles conferring late flowering at only two of the five loci. At the three other loci, H51 possessed alleles conferring early flowering in comparison to those of Landsbergerecta. Combinations of alleles conferring early and late flowering from both parents accounted for the transgressive segregation of flowering time observed within the F2 population. Three QTL,RLN1,RLN2 andRLN3 displayed significant genotype-by-environment interactions for flowering time. A significant interaction between alleles atRLN3 andRLN4 was detected.

Key words

Flowering time Vernalization Quantitative trait loci Arabidopsis RFLP 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Atherton JG (1987) Manipulation of flowering. Butterworths Scientific Publications, LondonGoogle Scholar
  2. Barthelmess I (1960) Merkmalskorrelation und Selektion beiArabidopsis thaliana (L.) Heynh. Z Pflanzenzucht 52:273–332Google Scholar
  3. Burn JE, Smyth DR, Peacock WJ, Dennis ES (1993) Genes conferring late flowering inArabidopsis thaliana. Genetica 90:147–155Google Scholar
  4. Chang C, Bowman JL, DeJohn AW, Lander ES, Meyerowitz EM (1988) Restriction fragment length polymorphism linkage map forArabidopsis thaliana. Proc Natl Acad Sci USA 85: 6856–6860PubMedGoogle Scholar
  5. Clarke JH, Dean C (1994) MappingFRI, a locus controlling flowering time and vernalization. Mol Gen Genet 242: 81–89PubMedGoogle Scholar
  6. De Vicente MC, Tanksley SD (1993) QTL analysis of transgressive segregation in an interspecific tomato cross. Genetics 134:585–596PubMedGoogle Scholar
  7. Dierks W (1958) Untersuchungen zum Heterosisproblem. Z Pflanzenzucht 40:67–102Google Scholar
  8. Haldane JBS (1919) The combination of linkage values, and the calculation of distances between the loci of linked factors. J Genet 8:299–309Google Scholar
  9. Härer L (1950) Die Verebung des Blähalters früher und später sommereinjähriger Rassen vonArabidopsis thaliana (L.) Heynh. Beitr Biol Pflanzen 28:1–35Google Scholar
  10. Koornneef M (1990)Arabidopsis thaliana In: O'Brian ST (ed) Genetic maps (locus maps of complex genomes) Book 6, Plants. Cold Spring Harbor Laboratory Press, Cold Spring Harbor New York, pp 6.94–6.97Google Scholar
  11. Koornneef M, Hanhart CJ, van der Veen JH (1991) A genetic and physiological analysis of late flowering mutants inArabidopsis thaliana. Mol Gen Genet 299:57–66Google Scholar
  12. Koornneef M, Blankestijin-de Vries H, Hanhart C, Soppe W, Peeters T (1994) The phenotype of some late-flowering mutants is enhanced by a locus on chromosome 5 that is not effective in the Landsbergerecta wild type. Plant J 6: 911–919Google Scholar
  13. Kowalski SP, Lan T-H, Feldmann KA, Paterson AH (1994) QTL mapping of naturally occurring variation in flowering time ofArabidopsis thaliana. Mol Gen Genet 245:548–555PubMedGoogle Scholar
  14. Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199PubMedGoogle Scholar
  15. Lander ES, Green P, Abrahamson J, Barlow J, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181PubMedGoogle Scholar
  16. Lee I, Bleeker A, Amasino R (1993) Analysis of naturally occurring late flowering inArabidopsis thaliana. Mol Gen Genet 237:171–176PubMedGoogle Scholar
  17. Lee I, Aukerman MJ, Gore SL, Lohman KN, Michaels SD, Weaver LM, John MC, Feldmann KA, Amasino RM (1994a) Isolation ofLUMINIDEPENDENS: a gene involved in the control of flowering time inArabidopsis. Plant Cell 6:75–83PubMedGoogle Scholar
  18. Lee I, Michaels SD, Masshardt AS, Amasino RM (1994b) The late flowering phenotype ofFRIGIDA and mutations inLUMINIDEPENDENS is suppressed in the Landsberg erecta strain of Arabidopsis. Plant J 6:903–909Google Scholar
  19. Lincoln SE, Lander ES (1990) Mapping genes controlling quantitative traits using MAPMAKER/QTL. A Whitehead Institute for Biomedical Research technical report, 1st ednGoogle Scholar
  20. Lister C, Dean C (1993) Recombinant inbred lines for the mapping of RFLP and phenotypic markers inArabidopsis thaliana. Plant J 4:745–750Google Scholar
  21. Meyerowitz EM (1987)Arabidopsis thaliana. Annu Rev Genet 21:93–111PubMedGoogle Scholar
  22. Napp-Zinn K (1957) Untersuchungen zur Genetik des Kältebedürfnisses beiArabidopsis thaliana. Z Indukt Abst Vererb 88:253–285Google Scholar
  23. Napp-Zinn K (1961) Über die Bedeutung genetischer Untersuchungen an kältebedürftigen Pflanzen für die Aufklärung von Vernalisationserscheinungen. Züchter 31:128–135Google Scholar
  24. Napp-Zinn K (1969)Arabidopsis thaliana (L.) Heynh In: Evans LT (ed) Introduction to flowering. MacMillan, Melbourne, pp 291–304Google Scholar
  25. Napp-Zinn K (1979) On the genetical basis of vernalization requirement inArabidopsis thaliana (L.) Heynh In: Physiologie de la floraison, No. 285. Editions du CNRS, pp 217–220Google Scholar
  26. Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complex linkage map of restriction fragment length polymorphisms. Nature 335:721–726PubMedGoogle Scholar
  27. Redei GP (1962) Supervital mutants ofArabidopsis. Genetics 47:433–460Google Scholar
  28. Redei GP (1992) A heuristic glance at the past ofArabidopsis genetics In: Koncz C, Chua N-H, Schell J (eds) Methods in tArabidopsis research. World Scientific, Singapore, pp 1–15Google Scholar
  29. Seyffert W (1960) Untersuchungen über die Vererbung quantitativer Charaktere anArabidopsis thaliana (L.) Heynh. Z Pflanzenzucht 42:356–401Google Scholar
  30. Stuber CW, Lincoln SE, Wolff DW, Helentjaris T, Lander ES (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics 132:823–839PubMedGoogle Scholar
  31. Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Jonathan H. Clarke
    • 1
  • Richard Mithen
    • 2
  • James K. M. Brown
    • 3
  • Caroline Dean
    • 1
  1. 1.Department of Molecular Genetics, BBSRCJohn Innes CentreColney LaneUK
  2. 2.Department of Brassicas and Oilseeds, BBSRCJohn Innes CentreColney LaneUK
  3. 3.Department of Cereal Research, BBSRCJohn Innes CentreColney LaneUK

Personalised recommendations