Photosynthesis Research

, Volume 37, Issue 2, pp 131–138 | Cite as

The relationship between Photosystem II intrinsic quantum yield and millisecond luminescence in thylakoids

  • D. Rees
  • P. Horton
  • U. Schreiber
Regular Paper


The relationship between charge recombination at Photosystem II (PS II), as indicated by millisecond luminescence, and PS II quantum yield was studied in spinach thylakoids during electron flow to methylviologen. Under the low magnesium conditions used, a decrease in quantum yield was observed in the absence of non-photochemical excitation quenching, and therefore cannot be due to a restriction in excitation delivery to the reaction centre. It was found that the decrease of the parameter Φp, which is a measure of the intrinsic quantum yield of ‘open’ PS II centers, correlates with an increase in luminescence per ‘open’ center. The relationship between these two parameters was the same whether Φp was manipulated by dissipation of the transthylakoid pH gradient or of the electrical potential. This indicates that the mechanism by which Φp decreases depends in the same way on the two components of the protonmotive force as does the charge recombination at PS II. Calculation of the yield of luminescence with respect to the back reaction will be necessary to determine whether the charge recombination occurs at a sufficiently high rate to be directly responsible for the Φp decrease.

Key words

luminescence Photosystem II quantum efficiency photosynthesis 



dark level chlorophyll fluorescence yield


maximum chlorophyll fluorescence vield


variable chlorophyll fluorescence yield (Fm-Fp)


reaction centre chlorophyll of PS II


Photosystem II


coefficient of non-photochemical fluorescence quenching


coefficient of photochemical fluorescence quenching


rate of O2 evolution/incident light

Φp−Φp/qp; QA

primary stable electron acceptor of PS II


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  1. Asada K, Neubauer C, Heber U and Schreiber U (1990) Methyl viologen-dependent cyclic electron transport in spinach chloroplasts in the absence of oxygen. Plant Cell Physiol 31: 557–564Google Scholar
  2. Barber J and Kraan GPB (1970) Salt-induced light emission from chloroplasts. Biochim Biophys Acta 197: 49–59PubMedGoogle Scholar
  3. Bjorkman O and Demmig B (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77K among vascular plants of diverse origin. Planta 170: 489–504Google Scholar
  4. Bradbury M and Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of Photosystem II electron acceptors and fluorescence emission from Photosystem I and II. Biochim Biophys Acta 63: 542–551Google Scholar
  5. Butler WL and Kitajima M (1975) Fluorescence quenching in PS 2 of chloroplasts. Biochim Biophys Acta 376: 116–125PubMedGoogle Scholar
  6. Delosme R (1967) Etude de l'induction de fluorescence des algues vertes et des chloroplasts au début d'une illumination intense. Biochim Biophys Acta 143: 108–128PubMedGoogle Scholar
  7. Demmig-Adams B (1990) Carotenoids and photoprotection in plants: A role for the xanthophyll zeaxanthin. Biochim Biophys Acta 1020: 1–24Google Scholar
  8. Genty B, Briantais JM and Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92Google Scholar
  9. Horton P and Hague A (1988) Studies on the induction of chlorophyll fluorescence in barley protoplasts. IV. Resolution of non-photosynthetic quenching. Biochim Biophys Acta 932: 107–115Google Scholar
  10. Horton P, Oxborough K, Rees D and Scholes JD (1988) Regulation of the photochemical efficiency of Photosystem 2: Consequences for the light response of field photosynthesis. Plant Physiol Biochem 26: 453–460Google Scholar
  11. Horton P, Ruban AV, Rees D, Pascal AA, Noctor GD and Young AJ (1991) Control of the light-harvesting function of chloroplast membranes by aggregation of the LHC II chlorophyll protein complex. FEBS Lett 292: 1–2PubMedGoogle Scholar
  12. Jensen RH and Bassham JA (1966) Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci USA 56: 1095–1101PubMedGoogle Scholar
  13. Jursinic PA (1986) Delayed fluorescence: Current concepts and status. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria, pp 291–328. Academic Press, OrlandoGoogle Scholar
  14. Lavorel J (1975) Luminescence. In: Govindjee(ed) Bioenergetics of Photosynthesis, pp 223–317. Academic Press, New YorkGoogle Scholar
  15. Malkin S (1977) Delayed luminescence. In: Barber J (ed) Primary Processes of Photosynthesis, pp 349–432. Elsevier, AmsterdamGoogle Scholar
  16. Mayne BC and Clayton RK (1966) Luminescence of chlorophyll in spinach chloroplasts induced by acid-base transition. Proc Natl Acad Sci USA 55: 494–497PubMedGoogle Scholar
  17. Neubauer C and Schreiber U (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: I. Saturation characteristics and partial control by the Photosystem II acceptor side. Z Naturforsch 42c: 1246–1254Google Scholar
  18. Ogren E and Baker NR (1985) Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light. Plant Cell Environ 8: 539–547Google Scholar
  19. Oxborough K and Horton P (1988) A study of the regulation and function of energy-dependent quenching in pea chloroplasts. Biochim Biophys Acta 548: 128–138Google Scholar
  20. Peterson RB, Sivak MN and Walker DA (1988) Relationship between steady-state fluorescence yield and photosynthetic efficiency in spinach leaf tissue. Plant Physiol 88: 158–163Google Scholar
  21. Quick P and Horton P (1984) Studies on the induction of chlorophyll fluorescence in barley protoplasts. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient. Proc R Soc Lond B 220: 371–382Google Scholar
  22. Rees D and Horton P (1990) The mechanism of changes in Photosystem 2 efficiency in spinach thylakoids. Biochim Biophys Acta 1016: 219–227Google Scholar
  23. Rees D, Noctor GD and Horton P (1990) The effect of high-energy-state excitation quenching on maximum and dark level chlorophyll fluorescence yield. Photosynth Res 25: 199–212Google Scholar
  24. Ruban AV, Rees D, Noctor GD, Young A and Horton P (1991) Long wavelength chlorophyll species are associated with amplification of high-energy-state excitation quenching in higher plants. Biochim Biophys Acta 1059: 335–360Google Scholar
  25. Schreiber U (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluoremeter. Photosynth Res 9: 261–272Google Scholar
  26. Schreiber U and Neubauer C (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: II. Partial control by the Photosystem II donor side and possible ways of interpretation. Z Naturforsch 42c: 1255–1264Google Scholar
  27. Schreiber U and Neubauer C (1989) Correlation between dissipative fluorescence quenching at Photosystem II and 50 μs recombination luminescence. FEBS Lett 258: 339–342Google Scholar
  28. Schreiber U and Neubauer C (1990) O2-dependent electron flow, membrane energization and the mechanism of non-photochemical quenching of chlorophyll fluorescence. Photosynth Res 25: 279–293Google Scholar
  29. Schreiber U and Rienits KG (1987) ATP-induced photochemical quenching of variable chlorophyll fluorescence. FEBS Lett 211: 99–104Google Scholar
  30. Schreiber U, Schiwa U and Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62Google Scholar
  31. Schreiber U and Schliwa U (1987) A solid-state portable instrument for measurement of chlorophyll luminescence induction in plants. Photosynthe Res 11: 173–182Google Scholar
  32. Sharkey TD, Berry JA and Sage RF (1988) Regulation of photosynthetic electron-transport inPhaseolus vulgaris L, as determined by room-temperature chlorophylla fluorescence. Planta 176: 415–424Google Scholar
  33. Weis E and Berry J (1987) Quantum efficiency of Photosystem II in relation to energy dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 198–208Google Scholar
  34. Wraight CA and Croft AR (1971) Delayed fluorescence and the high-energy state of chloroplasts. Eur J Biochem 19: 386–397PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • D. Rees
    • 2
  • P. Horton
    • 1
  • U. Schreiber
    • 2
  1. 1.Robert Hill Institute, Department of Molecular Biology and BiotechnologyUniversity of Sheffield, Western BankSheffieldUK
  2. 2.wheat program, Crop Management and PhysiologyCIMMYTMexico D.F.Mexico

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