Photosynthesis Research

, Volume 37, Issue 2, pp 131–138 | Cite as

The relationship between Photosystem II intrinsic quantum yield and millisecond luminescence in thylakoids

  • D. Rees
  • P. Horton
  • U. Schreiber
Regular Paper

Abstract

The relationship between charge recombination at Photosystem II (PS II), as indicated by millisecond luminescence, and PS II quantum yield was studied in spinach thylakoids during electron flow to methylviologen. Under the low magnesium conditions used, a decrease in quantum yield was observed in the absence of non-photochemical excitation quenching, and therefore cannot be due to a restriction in excitation delivery to the reaction centre. It was found that the decrease of the parameter Φp, which is a measure of the intrinsic quantum yield of ‘open’ PS II centers, correlates with an increase in luminescence per ‘open’ center. The relationship between these two parameters was the same whether Φp was manipulated by dissipation of the transthylakoid pH gradient or of the electrical potential. This indicates that the mechanism by which Φp decreases depends in the same way on the two components of the protonmotive force as does the charge recombination at PS II. Calculation of the yield of luminescence with respect to the back reaction will be necessary to determine whether the charge recombination occurs at a sufficiently high rate to be directly responsible for the Φp decrease.

Key words

luminescence Photosystem II quantum efficiency photosynthesis 

Abbreviations

Fo

dark level chlorophyll fluorescence yield

Fm

maximum chlorophyll fluorescence vield

Fv

variable chlorophyll fluorescence yield (Fm-Fp)

P680

reaction centre chlorophyll of PS II

PS II

Photosystem II

qN

coefficient of non-photochemical fluorescence quenching

qP

coefficient of photochemical fluorescence quenching

Φs

rate of O2 evolution/incident light

Φp−Φp/qp; QA

primary stable electron acceptor of PS II

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • D. Rees
    • 2
  • P. Horton
    • 1
  • U. Schreiber
    • 2
  1. 1.Robert Hill Institute, Department of Molecular Biology and BiotechnologyUniversity of Sheffield, Western BankSheffieldUK
  2. 2.wheat program, Crop Management and PhysiologyCIMMYTMexico D.F.Mexico

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