Journal of Applied Phycology

, Volume 6, Issue 1, pp 67–74 | Cite as

Effect of cell density and irradiance on growth, proximate composition and eicosapentaenoic acid production ofPhaeodactylum tricornutum grown in a tubular photobioreactor

  • Tjandra Chrismadha
  • Michael A. Borowitzka
Article

Abstract

Growth and eicosapentaenoic acid (EPA) productivity of the diatomPhaeodactylum tricornutum grown semicontinuously in a helical tubular photobioreactor were examined under a range of irradiances (approximately 56 to 1712 µmol photons m-2 s-1) and cell densities (≈3 × 106 to 18 × 106 cells mL-1). Self shading sets the upper limit of operational maximum cell density. Higher irradiance increases this upper limit and also increase the growth rate. Biomass productivity and EPA productivity were enhanced at those cell densities which support the fastest growth rate irrespective of irradiance. The cell protein content increased with increasing irradiance and the carbohydrate and lipid content increased with increasing cell density. EPA productivity was greatest at the highest irradiance. This study shows that biomass productivity and EPA productivity can be maximised by optimising cell density and irradiance, as well as by addition of CO2.

Key words

diatom lipid protein carbohydrate fatty acids eicosapentaenoic acid 

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References

  1. Alonso L, Grima EM, Perez JAS, Sanchez JLG, Camacho FG (1992) Fatty acid variation among different isolates of a single strain ofIsochrysis galbana. Phytochem. 31: 3901–3904.Google Scholar
  2. Becker EW (1984) Biotechnology and exploitation of the green algaScenedesmus obliquus in India. Biomass 4: 1–19.Google Scholar
  3. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.Google Scholar
  4. Bocci F, Torzillo G, Vincenzini M, Materassi R (1988) Growth physiology ofSpirulina platensis in tubular photobioreactor under natural light. In Stadler T, Mollion J, Verdus MC, Karamanos Y, Morvan H, Christiaen D (eds), Algal Biotechnology. Elsevier Applied Science, London: 219–228.Google Scholar
  5. Borowitzka MA (1988) Microalgae as sources of essential fatty acids. Aust. J. Biotechnol. 1: 58–62.Google Scholar
  6. Borowitzka MA, Volcani BE (1978) The polymorphic diatomPhaeodactylum tricornutum: Ultrastructure of its morphotypes. J. Phycol. 14: 10–21.Google Scholar
  7. Chrismadha T, Borowitzka MA (1993) Growth and lipid production ofPhaeodactylum tricornutum in a tubular photobioreactor. In Moi PS, Lee YK, Borowitzka MA, Whitton BA (eds), Algal Biotechnology in the Asia-Pacific Region. Institute of Advanced Studies, University of Malaya, Kuala Lumpur, (in press).Google Scholar
  8. Christie WW (1989) Gas Chromatography and Lipids. The Oily Press, Ayr, Scotland, 307 pp.Google Scholar
  9. Claustre H, Gostan J (1987) Adaptation of biochemical composition and cell size to irradiance in two microalgae: possible ecological implications. Mar. Ecol. Progr. Ser. 40: 167–174.Google Scholar
  10. Dauta A, Devaux J, Piquemal F, Boumnich L (1990) Growth rate of four freshwater algae in relation to light and temperature. Hydrobiologia 207 (Dev. Hydrobiol. 62): 221–226.Google Scholar
  11. Dunstan GA, Volkman JK, Barrett SM, Garland CD (1993) Changes in the lipid composition and maximisation of the polyunsaturated fatty acid content of 3 microalgae grown in mass culture. J. appl. Phycol. 5: 71–83.Google Scholar
  12. Fabregas J, Herrero C, Cabezas BV, Abalde J (1987) Growth and biochemical variability of the marine microalgaChlorella stigmatophora in batch cultures with different salinities and nutrient gradient concentration. Br. Phycol. J. 22: 269–276.Google Scholar
  13. Fawley MW (1984) Effects of light intensity and temperature interaction on growth characteristics ofPhaeodactylum tricornutum (Bacillariophyceae). J. Phycol. 20: 67–72.Google Scholar
  14. Goldman JC, Dennett MR, Riley CB (1981) Inorganic carbon sources and biomass regulation in intensive microalgal cultures. Biotechnol. Bioengng 23: 995–1014.Google Scholar
  15. Guillard RRL, Ryther JH (1962) Studies on marine planktonic diatoms. I.Cyclotella nana Hustedt andDetonula confervacea (Cleve) Gran. Can. J. Microbiol. 8: 229–239.Google Scholar
  16. Harrison PJ, Thompson PA, Calderwood GS (1990) Effects of nutrient and light limitation on the biochemical composition of phytoplankton. J. appl. Phycol. 2: 45–56.Google Scholar
  17. Holdsworth ES, Golbeck J (1976) The pattern of carbon fixation in the marine unicellular algaPhaeodactylum tricornutum. Mar. Biol. 38: 189–199.Google Scholar
  18. Javanmardian M, Palsson BO (1991) High-density photoautotrophic algal cultures — design, construction, and operation of a novel photobioreactor system. Biotechnol. Bioengng 38: 1182–1189.Google Scholar
  19. Kates M, Volcani BE (1966) Lipid components of diatoms. Biochim. Biophys. Acta 116: 264–278.Google Scholar
  20. Kochert G (1978) Carbohydrate determination by the phenol-sulphuric acid method. In Hellebust JA, Craigie JS (eds), Handbook of Phycological Methods. Physiological and Biochemical Methods. Cambridge University Press, Cambridge: 95–97.Google Scholar
  21. Lowrey OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J. biol. Chem. 1983: 265–275.Google Scholar
  22. Parrish CC, Wangersky PJ (1990) Growth and lipid class composition of the marine diatom,Chaetoceros gracilis, in laboratory and mass culture on turbidostats. J. Plankton Res. 12: 1011–1021.Google Scholar
  23. Rabe AE, Benoit J (1962) Mean light intensity — a useful concept in corellating growth rates of dense cultures of microalgae. Biotechnol. Bioengng 4: 377–390.Google Scholar
  24. Renaud SM, Parry DL, Thinh LV, Kuo C, Padovan A, Sammy N (1991) Effect of light intensity on the proximate biochemical and fatty acid composition ofIsochrysis sp. andNannochloropsis oculata for use in tropical aquaculture. J. appl. Phycol. 3: 43–53.Google Scholar
  25. Richmond A (1992) Open systems for the mass production of photoautotrophic microalgae outdoors — Physiological principles. J. appl. Phycol. 4: 281–286.Google Scholar
  26. Richmond A, Boussiba S, Vonshak A, Kopel R (1993) A new tubular reactor for mass production of microalgae outdoors. J. appl. Phycol. 5: 327–332.Google Scholar
  27. Robinson LF, Morrison AW, Bamforth MR (1988) Improvements relating to biosynthesis. European Patent Number 261, 872.Google Scholar
  28. Spectorova LV, Goronkova OI, Nosova LP, Albitskyaya ON (1981) High density culture of marine microalgae — Promising items for mariculture. I. Mineral feeding regime and installations for culturingDunaliella tertiolecta Butch. Aquacult. 26: 289–302.Google Scholar
  29. Spectorova LV, Nosova LP, Goronkova OI, Aibitskaya ON, Filippovskij YN (1986) High-density culture of marine microalgae — promising items for mariculture II. Determination of optimal light regime forChlorella sp.f. marina under high-density culture conditions. Aquacult. 55: 221–229.Google Scholar
  30. Sukenik A (1991) Ecophysiological considerations in the optimization of eicosapentaenoic acid production byNannochloropsis sp. (Eustigmatophyceae). Bioresource Technol. 35: 263–269.Google Scholar
  31. Sukenik A, Carmeli Y, Berner T (1989) Regulation of fatty acid composition by irradiance level in the EustigmatophyteNannochloropsis sp. J. Phycol. 25: 686–692.Google Scholar
  32. Tedesco MA, Duerr EO (1989) Light, temperature and nitrogen starvation effects on the total lipid and fatty acid content and composition ofSpirulina platensis UTEX1928. J. appl. Phycol. 1: 201–209.Google Scholar
  33. Terry KL, Hirata J, Laws EA (1983) Light-limited growth of two strains of the marine diatomPhaeodactylum tricornutum Bohlin: Chemical composition, carbon partitioning and the diel periodicity of physiological processes. J. exp. mar. Biol. Ecol. 68: 209–227.Google Scholar
  34. Thompson PA, Harrison PJ, Whyte JNC (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J. Phycol. 26: 278–288.Google Scholar
  35. Tredici MR, Materassi R (1992) From open ponds to vertical alveolar panels — The Italian experience in the development of reactors for the mass cultivation of phototrophic microorganisms. J. appl. Phycol. 4: 221–231.Google Scholar
  36. Tsuzuki M, Ohnuma E, Sato N, Takaku T, Kawaguchi A (1990) Effects of CO2 concentration during growth on fatty acid composition in microalgae. Plant Physiol. 93: 851–856.Google Scholar
  37. Veloso V, Reis A, Gouveia L, Fernandes HL, Empis JA, Novais JM (1991) Lipid production byPhaeodactylum tricornutum. Bioresource Technol. 38: 115–119.Google Scholar
  38. Vonshak A, Richmond A (1985) Problems in developing the biotechnology of algal mass production. Plant & Soil 89: 129–135.Google Scholar
  39. Weissman JC, Goebel RP (1987) Design and analysis of microalgal open pond systems for the purpose of producing fuels. Solar Energy Research Institute, Report SERI/STR-231-2840: 1–214.Google Scholar
  40. Yongmanitchai W, Ward OP (1991a) Growth of and omega-3 fatty acid production byPhaeodactylum tricornutum under different culture conditions. Appl. envir. Microbiol. 57: 419–425.Google Scholar
  41. Yongmanitchai W, Ward OP (1991b) Screening of algae for potential alternative sources of eicosapentaenoic acid. Phytochem. 30: 2963–2967.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Tjandra Chrismadha
    • 1
  • Michael A. Borowitzka
    • 1
  1. 1.Algal Biotechnology Laboratory, School of Biological and Environmental SciencesMurdoch UniversityPerthAustralia

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