The over-estimation of Fe2+ by a spectrophotometer due to simultaneous extraction of certain colouring matter in o-phenanthroline extracts can be avoided by making determinations on an atomic absorption spectrophotometer. It is shown that estimates of iron by atomic absorption spectrophotometer did not include measurable amount of Fe3+ due to a high specificity of o-phenanthroline for plant Fe2+.
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Katyal J C and Sharma B D 1980 Plant and Soil, 55, 105–119.
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Katyal, J.C., Sharma, B.D. Some modification in the assay of Fe2+ in 1–10, o-phenanthroline extracts of fresh plant tissues. Plant Soil 79, 449–450 (1984). https://doi.org/10.1007/BF02184337
- Fe2+, Fe3+
- Atomic absorption spectrophotometer