Plant and Soil

, Volume 73, Issue 3, pp 395–402 | Cite as

Isolation, purification and chemical composition of maize root cap slime

  • Annie Chaboud
Article

Summary

The total root exudate isolated axenically from roots is shown to constitute an extremely heterogenous population of particulate and soluble material. Differences in protein and total sugars contents, and neutral sugar composition throughout stages of total root exudate purification are reported. The importance of controlled collection and purification conditions to ensure valid analysis and composition of purified maize root cap slime are discussed.

Key words

Analysis Neutral sugar components Polysaccharides Purified root cap slime Zea mays L. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bowles D J and Northcote D H 1972 The sites of synthesis and transport of extracellular polysaccharides in the root tissues of maize. Biochem. J. 130, 1133–1145.PubMedGoogle Scholar
  2. 2.
    Burke D, Kaufman P, Mac Neil M and Albersheim P 1974 The structure of plant cell walls. VI A survey of the walls of suspension cultured monocots. Plant Physiol. 54, 109–115.Google Scholar
  3. 3.
    Chaboud A and Rougier M 1981 Sécrétions racinaires mucilagineuses et rôle dans la rhizosphère. Ann. Biol. 20, 313–326.Google Scholar
  4. 4.
    Clarke A E, Anderson R L and Stone B A 1979 Form and function of arabinogalactans and arabinogalactan proteins. Phytochem. 18, 521–540.Google Scholar
  5. 5.
    Clarke K J, MacCully M E and Miki N K 1979 A developmental study of the epidermis of young roots ofZea mays L. Protoplasma 98, 283–309.Google Scholar
  6. 6.
    Dubois M, Gilles K A, Hamilton J K, Rebers P A and Smith F 1956 Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356.Google Scholar
  7. 7.
    Floyd R A and Ohlrogge A J 1970 Gel formation on nodal root surface ofZea mays. I Investigation of the gel's composition. Plant and Soil 33, 331–343.Google Scholar
  8. 8.
    Foster R C 1982 The fine structure of epidermal cell mucilages of root. New Phytol. 91, 727–740.Google Scholar
  9. 9.
    Hall D, Mollenhauer H H and Morre D J 1966 Evidence for secretion of cell dispersing enzymes from maize root cap and epidermis. Am. J. Bot. 75, 65.Google Scholar
  10. 10.
    Harris P J and Northcote D H 1970 Patterns of polysaccharide biosynthesis in differentiating cells of maize root tips. Biochem. J. 120, 479–491.PubMedGoogle Scholar
  11. 11.
    Jenny H and Grossenbacher K 1963 Root-soil boundary zones as seen in the electron microscope. Proc. Soil. Sci. Soc. Am. 27, 273–277.Google Scholar
  12. 12.
    Jones D D and Morre D J 1967 Golgi apparatus mediated polysaccharide secretion by outer root cap cells ofZea mays. II Isolation and characterization of the secretory product. Z. Pflanzenphysiol. 56, 166–169.Google Scholar
  13. 13.
    Jones D D and Morre D J 1973 Golgi apparatus mediated polysaccharide secretion by outer root cap cells ofZea mays. III Control by exogenous sugars. Physiol. Plant. 29, 68–75.Google Scholar
  14. 14.
    Juniper B E and Roberts R M 1966 Polysaccharide synthesis and the fine structure of root cells. J. R. Microsc. Soc. 85, 63–72.Google Scholar
  15. 15.
    Kirby E G and Roberts R M 1971 The localized incorporation of3H fucose into cell wall polysaccharides of the cap and epidermis of corn roots. Autoradiographic and biosynthetic studies. Planta 99, 211–221.Google Scholar
  16. 16.
    Miki N K, Clarke K J and MacCully E M 1980 A histological and histochemical comparison of the mucilages on the root tips of several grasses. Can. J. Bot. 58, 2581–2593.Google Scholar
  17. 17.
    Northcote D H and Pickett Heaps J D 1966 A function of the Golgi apparatus in polysaccharide synthesis and transport in the root cap cells of wheat. Biochem. J. 98, 159–167.PubMedGoogle Scholar
  18. 18.
    Oades J M 1978 Mucilages at the root surface. J. Soil Sci. 29, 1–16.Google Scholar
  19. 19.
    Paull R E, Johnson C M and Jones R L 1975 Studies on the secretion of maize root cap slime. I Some properties of the secreted polymer. Plant Physiol. 56, 300–306.Google Scholar
  20. 20.
    Paull R E and Jones R L 1975 Studies on the secretion of maize root cap slime. III Histochemical and autoradiographic localization of incorporated fucose. Planta 127, 97–110.Google Scholar
  21. 21.
    Paull R E and Jones R L 1976 Studies on the secretion of maize root cap slime. V The cell wall as a barrier to secretion. Z.Pflanzenphysiol. 79, 154–164.Google Scholar
  22. 22.
    Rougier M 1976 Sécrétion de polysaccharides dans l'apex radiculaire de maīs: étude radioautographique par incorporation de fucose tritié. J. Microsc. 26, 161–166.Google Scholar
  23. 23.
    Rougier M 1981 Secretory activity of the root cap.In Encyclopedia of Plant Physiology, New Series, Plant Carbohydrates, II, vol. 13B. Eds W Tanner and F A Loewus, pp 542–574.Google Scholar
  24. 24.
    Rovira A D, Foster R C and Martin J K 1979 Origin, nature and nomenclature of the organic materials in the rhizosphere.In The Soil-Root Interface. Eds JL Harley and R Scott Russell. Academic Press, pp 1–4.Google Scholar
  25. 25.
    Rovira A D and MacDougall B M 1967 Microbiological and biochemical aspects of the rhizosphere.In Soil Biochemistry. Eds AD MacLaren and GH Petersen. M Deckker Inc. pp 417–463.Google Scholar
  26. 26.
    Sawardeker J S, Sloneke R and Jeanes A 1965 Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography. Anal. Chem. 37, 1602–1604.Google Scholar
  27. 27.
    Sedmak J J and Grossberg S E 1977 A rapid sensitive and versatile assay for protein using Coomassie Brillant Blue G 250. Anal. Biochem. 79, 544–552.PubMedGoogle Scholar
  28. 28.
    Schönwitz R and Ziegler H 1982 Exsudation of water soluble vitamins and of some carbohydrates by intact roots of maize seedlings (Zea mays L.) into a mineral nutrient solution. Z. Pflanzenphysiol. 107, 7–14.Google Scholar
  29. 29.
    Werker E and Kislev M 1978 Mucilage on the root surface and root hairs ofSorghum: heterogeneity in structure, manner of production and site of accumulation. Ann. Bot. 42, 809–816.Google Scholar
  30. 30.
    Wright K 1975 Polysaccharides of root-cap slime from five varieties. Phytochem. 14, 759–763.Google Scholar
  31. 31.
    Wright K and Northcote D H 1974 The relationship of root cap slimes to pectins. Biochem. J. 139, 525–534.PubMedGoogle Scholar
  32. 32.
    Wright K and Northcote D H 1975 An acidic oligosaccharide from maize slime. Phytochem. 14, 1793–1798.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1983

Authors and Affiliations

  • Annie Chaboud
    • 1
  1. 1.Departement de Biologie Végètale, Reconnaissance Cellulaire et Amélioration des PlantesUniversité LyonVilleurbanne CedexFrance

Personalised recommendations