Plant and Soil

, Volume 161, Issue 1, pp 91–96 | Cite as

Sucrose transport and hydrolysis inRhizobium tropici

  • Vasilly I. Romanov
  • Esperanza Martínez-Romero


TheRhizobium tropici strain CFN 299 was maintained on PY medium and was grown in minimal medium (MM) with sucrose, glucose, fructose and glutamate (or their combination) as carbon sources. Bacteria were able to simultaneously use different carbon sources and, with a combination sucrose and glutamate, the growth rate was faster than with either carbon source alone. Sucrose transport was induced by sucrose and partially repressed by glucose and glutamate if they were included in MM as additional carbon sources. The transport of sucrose was active because both an uncoupler (dinitrophenol, DNP) and inhibitors of terminal oxidation (KCN, NaN3) severely reduced sucrose uptake. Sucrose transport was also sensitive to a functional sulfhydryl reagent but was much less sensitive to EDTA and arsenate. We obtained nonlinear Lineweaver-Burk plots for the uptake of sucrose (by sucrose-grown bacteria), and this implied the existence of at least two uptake mechanisms. Invertase (EC is the main enzyme for sucrose hydrolysis in this organism. This enzyme was induced by sucrose and had high activity in mid-log phase cells when sucrose was the sole carbon source (0.2%). Invertase activity was not detected in growth medium. In general, the results obtained support the idea, thatR. tropici is adapted to sucrose utilization and to multicarbon nutrition during its interaction with plants.

Key words

catabolite repression Rhizobium sucrose transport 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Day D A and Copeland L 1991 Carbon metabolism and compartmentation in nitrogen fixing legume nodules. Plant Physiol. Biochem. 29, 185–201.Google Scholar
  2. De Hollander J A and Stouthamer A H 1979 Multicarbon-substrate growth ofRhizobium trifolii. FEMS Microbiol. Let. 6, 57–59.Google Scholar
  3. Duncan M J 1981 Properties of Tn5-induced carbohydrate mutants inRhizobium meliloti. J. Gen. Microbiol. 122, 61–67.Google Scholar
  4. Glenn A R and Dilworth M J 1981 The uptake and hydrolysis of disaccharides by fast and slow-growing species ofRhizobium. Arch. Microbiol. 129, 233–239.Google Scholar
  5. Herrada G, Puppo A and Rigaud J 1989 Uptake of metabolites by bacteroid-containing vesicles and by free bacteroids from french bean nodules. J. Gen. Microbiol. 135, 3165–3171.Google Scholar
  6. Hoelzle I and Streeter J G 1990 Stimulation of α-glucosidases from fast-growing rhizobia andAgrobacterium tumefaciens by K+, NH4 +, and Rb+. Can. J. Microbiol. 36, 223–227.Google Scholar
  7. Martínez-Romero E, Segovia L, Mercante F M, Franco A A, Graham P and Pardo M A 1991Rhizobium tropici, a novel species nodulatingPhaseolus vulgaris L. beans andLeucaena spp. trees. Int. J. Syst. Bacteriol. 41, 417–426.PubMedGoogle Scholar
  8. Mackay I A, Dilworth M J and Glenn A R 1989 Carbon catabolism in continuous culture and bacteroids of Rhizobium leguminosarum MNF 3841. Arch. Microbiol. 152, 606–610.Google Scholar
  9. Mieyal J J 1972 Sucrose phosphorylase fromPseudomonas saccharophila. Methods in Enzymology 28, 935–943.Google Scholar
  10. Noel K D, Sanchez A, Fernandez L, Leemans J and Cevallos M A 1984Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions. J. Bacteriol. 158, 148–155.PubMedGoogle Scholar
  11. Romanov V I, Hajy-sadeh B R, Ivanov B F, Shaposhnikov G L and Kretowich W L 1985 Labelling of lupine nodule metabolites with14CO2 assimilated from the leaves. Phytochemistry 24, 2157–2160.Google Scholar
  12. Streeter J G 1991 Transport and metabolism of carbon and nitrogen in legume nodules. Adv. Bot. Res. 18, 129–187.Google Scholar
  13. Thummer F and Verma D P S 1987 Nodulin-100 of soybean is the subunit of sucrose synthase regulated by the availability of free heme in nodules. J. Biol. Chem. 262, 14730–14736.PubMedGoogle Scholar
  14. Vance C P and Hiechel G H 1991 Carbon in N2 fixation:Limitation and exquisite adaptation. Annu. Rev. Plant Physiol. 42, 373–392.Google Scholar
  15. Wong T Y 1990 Possible mechanism of mannose inhibition of sucrose-supported growth in N2-fixingAzotobacter vinelandii. Appl. Environ. Microbiol. 56, 93–97.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Vasilly I. Romanov
    • 1
    • 2
  • Esperanza Martínez-Romero
    • 1
    • 2
  1. 1.A.N.Bach Institute of Biochemistry RASMoscowRussia
  2. 2.CIFN-UNAMCuernavaca, Mor.México

Personalised recommendations