Journal of Applied Phycology

, Volume 6, Issue 4, pp 431–433 | Cite as

Development of media for the mixotrophic/heterotrophic culture ofBrachiomonas submarina

  • Alexander J. Tsavalos
  • John G. Day


The marine algaBrachiomonas submarina var.pulsifera (Droop) CCAP 7/2A, is employed as a food organism in aquaculture; it can be cultured heterotrophically or mixotrophically. Growth rates and productivity under heterotrophic conditions were lower than those achieved under mixotrophic conditions. By reducing the osmotic potential of the medium, whilst simultaneously increasing the levels of nitrogen and phosphorus and using sodium acetate as a carbon source, a 20-fold increase in final yield was attained. This corresponded to a maximum culture of 9.02times 106 cells ml−1 and a dry weight of 2.51 g l−1.

Key words

Brachiomonas biotechnology heterotrophic/mixotrophic culture aquaculture 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Alexander J. Tsavalos
    • 1
  • John G. Day
    • 2
  1. 1.School of Biological and Earth SciencesLiverpool John Moores UniversityLiverpoolUK
  2. 2.Culture Collection of Algae and ProtozoaInstitute of Freshwater Ecology, Windermere LaboratoryAmblesideUK

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