The exact number of replicates required for a given probability of isolating all the species of nematophagous fungi present in a single sample of soil was measured. Using the soil sprinkling technique twenty replicate sub-samples of a sample of farm soil were examined for species. Eight replicates were required under these circumstances to be 95% sure of isolating all the species present. An equation to calculate the number of replicates to achieve a given probability of 100% species isolation is also given.
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Bailey, F., Gray, N.F. The effect of replication on the probability of isolating nematophagous fungi from soil. Plant Soil 113, 141–143 (1989). https://doi.org/10.1007/BF02181934
- nematophagous fungi
- sampling procedure