In sediment slurry experiments with anoxic marine sediments collected in Cape Lookout Bight, NC, and a site in mid-Chesapeake Bay, the rates of sulfate reduction and ammonium production decrease with increasing dilution of sediment with oxygen-free sea-water. The effect of sediment dilution on the rates of these processes can be described by a simple mathematical relationship, and when these rates are corrected for sediment dilution they yield values which agree well with direct measurements of these processes.
In sediment slurry studies of amino acid utilization in Cape Lookout Bight sediments, the fermentative decarboxylation of glutamic acid (to γ-aminobutyric acid) or aspartic acid (to alanine or β-alanine) did not occur when either of these amino acids were added to Cape Lookout Bight slurries. The addition of glutamic acid did however lead to a small (∼1) transient build-up of β-aminoglutaric acid. Measured rates of glutamic acid uptake in these slurries also decreased with increasing sediment dilution.
Molybdate inhibition experiments demonstrated that dissolved free amino acids represent 1–3% of the carbon sources/electron donors used for sulfate reduction in Cape Lookout Bight sediments. The direct oxidation of amino acids by sulfate reducing bacteria also accounts for 13–20% of the total ammonium produced. Glutamic acid, alanine, β-aminoglutaric acid, aspartic acid and asparagine are the major amino acids oxidized by sulfate reducing bacteria in Cape Lookout Bight sediments.
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Aller RC & Yingst JC (1980) Relationships between microbial distributions and the anaerobic decomposition of organic matter in surface sediments of Long Island Sound, USA. Mar. Biol. 56: 29–42
Alperin MJ & Reebuirgh WS (1985) Inhibition experiments on anaerobic methane oxidation. Appl. Environ. Microbiol. 50: 940–945
Banat IM, Lindstrom EB, Nedwell DB & Balba MT (1981) Evidence for the existence of two distinct functional groups of sulfate-reducing bacteria in salt marsh sediments. Appl. Environ. Microbiol. 42: 985–992
Barker HA (1981) Amino acid degradation by anaerobic bacteria. Ann. Rev. Biochem. 50: 23–40
Berner RA (1980) Early Diagenesis, A theoretical approach. Princeton University Press: Princeton, N.J.
Boudreau BP & Westrich JT (1984) The dependence of bacterial sulfate reduction on sulfate concentrations in marine sediments. Geochim. Cosmochim. Acta 48: 2503–2516
Burdige DJ (1988) The microbial utilization of alanine and glutamic acid in anoxic marine sediments. EOS 69: 1122
Burdige DJ & Martens CS (1984) Amino acid cycling in an organic-rich sediment. EOS 65: 960
Burdige DJ & Martens CS (1988) Biogeochemical cycling in an organic-rich marine basin — 10. The role of amino acids in sedimentary carbon and nitrogen cycling. Geochim. Cosmochim. Acta 52: 1571–1584
Chanton JP, Martens CS & Kipphut GW (1983) Lead-210 sediment geochronology in a changing coastal environment. Geochim. Cosmochim. Acta 47: 1791–1804
Christensen D (1984) Determination of substrates oxidized by sulfate reduction in intact cores of marine sediments. Limnol. Oceanogr. 29: 189–192
Coleman GS (1960) A sulfate reducing bacterium from the sheep rumen. J. Gen. Microbiol. 22: 423–436
Crill PM & Martens CS (1983) Spatial and temporal fluctuations of methane production in anoxic coastal marine sediments. Limnol. Oceanogr. 28: 1117–1130
Crill PM & Martens CS (1987) Biogeochemical cycling in an organic-rich marine basin — 6. Temporal and spatial variations in sulfate reduction rates. Geochim. Cosmochim. Acta. 51: 1175–1186
Degens ET, Reuter JH & Shaw KNF (1964) Biochemical compounds in offshore California sediments and sea waters. Geochim. Cosmochim. Acta 28: 45–66
Doyle AP & Henrichs SM (1986) Decomposition of amino acids in marine sediments. EOS 67: 1043
Gottschalk G (1979) Microbial Metabolism. Springer-Verlag
Hedges JI & Hare PE (1987) Amino acid adsorption by clay minerals in distilled water. Geochim. Cosmochim. Acta 51: 255–259
Henrichs SM & Farrington JW (1979) Amino acids in interstitial waters of marine sediments. Nature 279: 319–322
Henrichs SM & Farrington JW (1987) Early diagenesis of amino acids and organic matter in two coastal marine sediments. Geochim. Cosmochim. Acta 51: 1–15
Henrichs SM, Farrington JW & Lee C (1984) Peru upwelling region sediments near 15°S. 2. Dissolved free and total hydrolyzable amino acids. Limnol. Oceanogr. 29: 20–34
Jørgensen BB (1978) A comparison of methods for the quantification of bacterial sulfate reduction in coastal marine sediments. 1. Measurements with radiotracer techniques. Geomicrobiol. J. 1: 11–27
King GM (1984) Metabolism of trimethylamine, choline, and glycine betaine by sulfate-reducing and methanogenic bacteria in marine sediments. Appl. Environ. Microbiol. 48: 719–725
Klump JV & Martens CS (1989) The seasonality of nutrient regeneration in an organic rich coastal sediment: Kinetic modelling of changing pore water nutrient and sulfate distributions. Limnol Oceanogr. In press
Laanbroek HJ & Veldkamp H (1982) Microbial interactions in sediment communities. Phil. Trans. Royal Soc. London B 297: 533–550
Lindroth P & Mopper K (1979) HPLC determination of subpicomole amounts of amino acids by precolumn fluorescence derivitization with o-phthaldialdehyde. Anal. Chem. 51: 1667–1674
Mackin JE & Aller RC (1984) Ammonium adsorption in marine sediments. Limnol. Oceanogr. 29: 250–257
Martens CS & Klump JV (1984) Biogeochemical cycling in an organic-rich marine basin — 4. An organic carbon budget for sediments dominated by sulfate reduction and methanogenesis. Geochim. Cosmochim. Acta 48: 1987–2004
Matisoff G, Bricker OP III, Holdren GR Jr & Kaerk P (1975) Spatial and temporal variations in the interstitial water chemistry of Chesapeake Bay sediments. In: Church TM (Ed) Marine Chemistry in the Coastal Environment (pp 343–363). ACS Symposium ser. 18
McNichol AP (1986) A study of the remineralization of organic carbon in nearshore sediments using carbon isotopes. PhD thesis MIT/WHOI
Oremland RS & Taylor BF (1978) Sulfate reduction and methanogenesis in marine sediments. Geochim. Cosmochim. Acta 42: 209–214
Roden EE & Tuttle JH (1987) Regional variations in sulfur cycling in Chesapeake Bay sediments. EOS 68: 1757
Rosenfeld JK (1979) Ammonium adsorption in nearshore anoxic sediments. Limnol. Oceanogr. 24: 356–364
San Diego-McGlone, M.L. & Cutter GA (1988) Processes affecting redox sensitive elements in Chesapeake Bay. EOS 69: 375
Sansone FJ (1986) Depth distribution of short-chain organic acid turnover in Cape Lookout Bight sediments. Geochim. Cosmochim. Acta 50: 99–105
Sansone FJ & Martens CS (1982) Volatile fatty acid cycling in organic-rich marine sediments. Geochim. Cosmochim. Acta 45: 101–121
Skyring GW, Jones HE & Goodchild D (1977) The taxonomy of some new isolates of dissimilatory sulfate-reducing bacteria. Canad. J. Microbiol. 23: 1415–1425
Smith RL & Klug MJ (1981) Electron donors utilized by sulfate-reducing bacteria in eutrophic lake sediments. Appl. Environ. Microbiol. 42: 116–121
Solorzano L (1969) Determination of ammonia in natural waters by the phenol-hypochlorite method. Limnol. Oceanogr. 14: 799–801
Sørensen J, Christensen D & Jørgensen BB (1981) Volatile fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediments. Appl. Environ. Microbiol. 42: 5–11
Stams AJM & Hansen TA (1984) Fermentation of glutamate and other compounds byAcidaminobacter hydrogenoformans gen. nov. sp. nov., an obligate anaerobe isolated from black mud. Studies with pure cultures and mixed cultures with sulfate-reducing and methanogenic bacteria. Arch. Microbiol. 137: 329–337
Stams AJM & Hansen TA (1986) Metabolism of L-alanine inDesulfotomaculum ruminis and two marineDesulfovibrio strains. Arch. Microbiol. 145: 277–279
Stams AJM, Hansen TA & Skyring GW (1985) Utilization of amino acids as energy substrates by two marineDesulfovibrio strains. FEMS Microbiol. Lett. 31: 11–15
Tabatabai MA (1974) A rapid method for determination of sulfate in water samples. Environ. Letter 7: 237–243
Whelan JK (1977) Amino acids in a surface sediment core of the Atlantic abyssal plain. Geochim. Cosmochim. Acta 41: 803–810
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Burdige, D.J. The effects of sediment slurrying on microbial processes, and the role of amino acids as substrates for sulfate reduction in anoxic marine sediments. Biogeochemistry 8, 1–23 (1989). https://doi.org/10.1007/BF02180164
- amino acids
- ammonium production
- coastal marine sediments
- sulfate reduction