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Molecular and General Genetics MGG

, Volume 252, Issue 6, pp 746–750 | Cite as

A vital function for mitochondrial DNA in the petite-negative yeastKluyveromyces lactis

  • G. D. Clark-Walker
  • X. J. Chen
Short Communication

Abstract

Petite-negative yeasts do not form viable respiratory-deficient mutants on treatment with DNA-targeting drugs that readily eliminate the mitochondial DNA (mtDNA) from petite-positive yeasts. However, in the petite-negative yeastKluyveromyces lactis, specific mutations in the nuclear genesMGI2 andMGI5 encoding theα- andγ-subunits of the mitochondrial F1-ATPase, allow mtDNA to be lost. In this study we show that wild-typeK. lactis does not survive in the absence of its mitochondrial genome and that the function ofmgi mutations is to suppress lethality caused by loss of mtDNA. Firstly, we find that loss of a multicopy plasmid bearing amgi allele readily occurs from a wild-type strain with functional mtDNA but is not tolerated in the absence of mtDNA. Secondly, we cloned theK. lactis homologue of theSaccharomyces cerevisiae mitochondrial genome maintenance geneMGM101, and disrupted one of the two copies in a diploid. Following sporulation, we find that segregants containing the disrupted gene form minicolonies containing 6-8000 inviable cells. By contrast, disruption ofMGM101 is not lethal in a haploidmgi strain with a specific mutation in a subunit of the mitochondrial F1-ATPase. These observations suggest that mtDNA inK. lactis encodes a vital function which may reside in one of the three mitochondrially encoded subunits of F0.

Key words

Mitochondrial DNA Kluyveromyces lactis MGM101/MGI genes Petite-negative yeast 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • G. D. Clark-Walker
    • 1
  • X. J. Chen
    • 1
  1. 1.Molecular and Population Genetics Group, Research School of Biological SciencesThe Australian National UniversityCanberra CityAustralia

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