Molecular and General Genetics MGG

, Volume 252, Issue 1–2, pp 87–92

Genetic analysis of two tomato mutants affected in the regulation of iron metabolism

  • Hong-Qing Ling
  • Axel Pich
  • Günter Scholz
  • Martin W. Ganal
Original Paper

DOI: 10.1007/BF02173208

Cite this article as:
Ling, HQ., Pich, A., Scholz, G. et al. Molec. Gen. Genet. (1996) 252: 87. doi:10.1007/BF02173208

Abstract

Iron is one of the most important micronutrients for plants. Like other organisms, plants have developed active mechanisms for the acquisition of sufficient iron from the soil. Nevertheless, very little is known about the genetic mechanisms that control the active uptake. In tomato, two spontaneously derived mutants are available, which are defective in key steps that control this process. The recessive mutationchloronerva (chln) affects a gene which controls the synthesis of the non-protein amino acid nicotianamine (NA), a key component in the iron physiology of plants. The root system of the recessive mutantfer is unable to induce any of the characteristic responses to iron deficiency and iron uptake is thus completely blocked. We present a characterization of the double mutant, showing that thefer gene is epistatic over thechln gene and thus very likely to be one of the major genetic elements controlling iron physiology in tomato. In order to gain access to these two genes at the molecular level, both mutants were precisely mapped onto the high density RFLP map of tomato. Thechln gene is located on chromosome 1 and thefer gene is on chromosome 6 of tomato. Using this high-resolution map, a chromosome walk has been started to isolate thefer gene by map-based cloning. The isolation of thefer gene will provide new insights into the molecular mechanisms of iron uptake control in plants.

Key words

Lycopersicon esculentum Genetic mapping RFLP RAPD Plant nutrition 

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Hong-Qing Ling
    • 1
  • Axel Pich
    • 1
  • Günter Scholz
    • 1
  • Martin W. Ganal
    • 1
  1. 1.Institut für Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany

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