Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

gaMS-1: A gametophytic male sterile mutant in maize

Abstract

Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization ofgametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis.gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, nonfunctional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.

This is a preview of subscription content, log in to check access.

References

  1. Aarts MGM, Dirkse WG, Stlekema WJ, Pereira A (1993) Transposon tagging of a male sterility gene inArabidopsis. Nature 363:715–717

  2. Bedinger PA, Broadwater AH, Conway JD, Hardeman KJ, Lonkides CA, Prata RTN, Rubinstain AL (1994) Molecular studies of pollen development in maize. Curr Top Plant Physiol 12: 1–14

  3. Burnham CR (1982) Details of the smear technique for studying chromosomes in maize. In: Sheridan W (ed) Maize for biological research. Plant Molecular Biology Association, Charlottesville, Va, pp 107–118

  4. Chang MT, Neuffer MG (1989) Maize microsporogenesis. Genome 32:232–244

  5. Chaudhury AM (1993) Nuclear genes controlling male fertility. Plant Cell 5:1277–1283

  6. Eady C, Lindsey K, Twell D (1994) Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen. Plant J 5:543–550

  7. Eady C, Lindsey K, Twell D (1995) The significance of microspore division and division symmetry for vegetative cell-specific transcription and generative cell differentiation. Plant Cell 7:65–74

  8. Hirata J, Nakagoshi H, Nabeshima Y, Matsuzaki F (1995) Asymmetric segregation of the homeodomain protein Prospero duringDrosophila development. Nature 377:627–630

  9. Horvitz RH, Herskowitz I (1992) Mechanisms of asymmetric cell division. Two Bs or not two Bs, that is the question. Cell 68:237–255

  10. Kindiger B, Beckett TA (1985) A hematoxylin staining procedure for maize pollen grain chromosomes. Stain Technol 60: 265–270

  11. Kindiger B, Beckett TA, Coe EH (1991) Differential effects of specific chromosomal deficiencies on the development of the maize pollen grain. Genome 32:579–594

  12. Knoblich K, Jan LJ, Nung Jan Y (1995) Asymmetric segregation of Numb and Prospero during cell division. Nature 377:624–627

  13. Laughnan JR, Gabay SJ (1973) Reaction of germinating maize pollen toHelminthosporium maydis pathotoxins. Crop Sci 43:681–684

  14. Mangelsdorf PC (1932) Mechanical separation of gametes in maize. J Hered 23:288–295

  15. Mascarenhas JP (1990) Gene activity during pollen development. Annu Rev Plant Physiol 41:317–338

  16. McCormick S (1993) Male gametophyte development. Plant Cell 5:1265–1275

  17. McCormick S, Chen S, Curie C, Eyal Y, Kulikauskas R (1994) Molecular and genetic analyses of pollen development. Curr Top Plant Physiol 12:29–38

  18. Muschietti J, Dirks L, Vancanneyt G, McCormick S (1994) LAT52 protein is essential for tomato pollen development: pollen expressing antisense LAT52 RNA hydrates and germinates abnormally and cannot achieve fertilization. Plant J 6:321–338

  19. Sari-Gorla M, Pe ME, Mulcahy DL, Ottaviano E (1992) Genetic dissection of pollen competitive ability in maize. Heredity 69:423–430

  20. Sari-Gorla M, Pè ME, Rossini L (1994) Detection of QTLs controlling pollen germination and growth in maize. Heredity 72:332–335

  21. Sari-Gorla M, Binelli G, Pè ME, Villa M (1995) Detection of genetic factors controlling pollen-style interaction in maize. Heredity 74:62–69

  22. Twell D, Wing R, Yamaguchi J, McCormick S (1989) Isolation and expression of an anther-specific gene from tomato. Mol Gen Genet 217:240–245

Download references

Author information

Correspondence to M. Sari-Gorla.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sari-Gorla, M., Ferrario, S., Villa, M. et al. gaMS-1: A gametophytic male sterile mutant in maize. Sexual Plant Reprod 9, 216–220 (1996). https://doi.org/10.1007/BF02173101

Download citation

Key words

  • Maize pollen
  • Male sterility
  • Microsporogenesis
  • Gametophytic gene expression